In vitro storage of plum germplasm by slow growth

Giannì, Sergio; Sottile, Francesco

doi:10.17221/186/2014-hortsci

Cited by 27 publications

(18 citation statements)

References 31 publications

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“…3 and 4c). Contrary to our results, two Italian P. domestica genotypes survived cold storage markedly longer, for 12 and 9 months, while P. cerasifera ones remained viable for up to 6 months (Gianní and Sottile, 2015), similar to our results for 'Crvena Ranka' shoots.…”

Section: Resultssupporting

confidence: 77%

In vitro propagation and medium-term conservation of autochthonous plum cultivar 'Crvena Ranka'

Vujović¹,

Jevremović²,

Marjanović³

et al. 2020

Acta agriculturae Serbica

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In vitro strategies for the propagation and conservation of indigenous species contribute to the sustainable use of plant diversity and are essential for breeding programs as well. In this study, we established an efficient protocol for the micropropagation of autochthonous plum 'Crvena Ranka' and examined the survival and regrowth capacity of in vitro shoots after 3, 6 and 9 months of cold storage (CS) at +5 oC in total darkness. Aseptic culture was established on the Murashige and Skoog medium containing 2 mg l-1 BA, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3 (leaf rosette initiation being 68.8%). During in vitro propagation on the medium of constant hormonal composition, a significant increase in the multiplication index was observed in the third subculture, whereupon it was mainly stable until the fifth subculture. The effect of BA concentration and/or type of auxins (IBA or NAA) on multiplication parameters, as well as on fresh and dry weights of shoots was evaluated. BA at 1 mg l-1 in combination with NAA significantly increased shoot multiplication parameters. The effect of auxins on rooting parameters was monitored as well. Shoots cultured on the medium supplemented with NAA also displayed higher rooting ability (60%), in comparison with those grown on the medium containing IBA at the same concentration (20%). In vitro shoots can be conserved over the medium term under CS conditions up to six months. High survival was achieved after three (94%) and six months (82.5%), while severe signs of necrosis (100%) were noticed after nine months of conservation. Shoots subcultured under standard growth conditions after CS promptly regained their morphology although their capacity for multiplication and rooting was slightly lower than that of non-cold-stored shoots.

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Section: Resultssupporting

confidence: 77%

In vitro propagation and medium-term conservation of autochthonous plum cultivar 'Crvena Ranka'

Vujović¹,

Jevremović²,

Marjanović³

et al. 2020

Acta agriculturae Serbica

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“…Ariddu di Core and Sanacore but less so for the cv. Marabolo and Rapparino varieties (Gianní and Sottile 2015). Darkness had a positive effect on the regrowth of the Vitis berlandieri × V. riparia after 3, 6, and 9 months of 4 °C storage in alginate beads (Benelli 2016).…”

Section: Discussionmentioning

confidence: 93%

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

Kamińska

Gołębiewski

Tretyn

et al. 2017

Plant Cell Tiss Organ Cult

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The aim of this paper was to develop a protocol for efficient storage of artificial seeds of Taraxacum pieninicum, critically endangered Asteraceae species. Storage under reduced light conditions or in the darkness was tested on a basis of synthetic seeds ability to conversion and post-storage regrowth of shoot tips. The results indicated that synseeds obtained from shoot tips of T. pieninicum can be stored at 4 °C even for 12 months without subculture. The light is a stress factor during storage what was manifested by numerous necrosis and decreased shoots ability to proliferate in optimal growth conditions in 1st subculture. Additionally our results showed that the storage does not produce genetic variation at the resolution provided by the flow cytometry and RAPD analysis.

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“…Slow growth is a very simple method for germplasm conservation and has been reported in several studies [35,[44][45][46]. Divakaran et al [45] demonstrated that addition of mannitol (10-15 g/L) and reduction of sucrose to lower level (15-10 g/L) induced slow growth of Vanilla planifolia cultures and subsequently 80%-90% of the cultures could be maintained for a period of 360 days.…”

Section: Establishment Of In Vitro Propagationmentioning

confidence: 98%

Ex situ preservation for some endemic and rare medicinal plants in Taif, KSA

Attia

Dessoky

Al-Sodany

et al. 2017

Biotechnology & Biotechnological Equipment

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Medium-term storage for some medicinal plant species collected from Taif governorate was developed. First, the establishment of in vitro propagation system for Caper (Capparis spinosa L) and Lavender (Lavandula dentata L.) plants was studied using axillary buds as explants. Murashige and Skoag (MS) salts with different concentrations and combinations of auxins and cytokinens were used. Second, Shoot tips and nodal buds from in vitro culture plants (C. spinosa L, L. dentata L and Rhazya stricta Decne) were used as explants for in vitro conservation experiments. Eighteen different treatments were used as slow growth medium. After 12 months from conservation, in R. stricta Decne and C. spinosa L a highest percentage of survival rates (91.1% and 93.33%), respectively, were observed on MS + 10 g/L sucrose + 10 g/L sorbitol. However, in L. dentata L the highest percentage of survival rate (90%) was noted on MS + 15 g/L sucrose +10 g/L sorbitol. Random amplified polymorphic DNA (RAPD) analysis indicated high genetic stability of preserved plant species under investigation. These results suggested that, in vitro conservation using full strength MS salts with low concentrations of sucrose and sorbitol as a carbon source and osmotic agent, respectively, is a suitable slow growth medium for in vitro conservation of C. spinosa L, L. dentata and R. stricta Decne plants.

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In vitro storage of plum germplasm by slow growth

Cited by 27 publications

References 31 publications

In vitro propagation and medium-term conservation of autochthonous plum cultivar 'Crvena Ranka'

In vitro propagation and medium-term conservation of autochthonous plum cultivar 'Crvena Ranka'

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

Ex situ preservation for some endemic and rare medicinal plants in Taif, KSA

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