In vitro Storage of Strawberry and Raspberry in Calcium-Alginate Beads at 4°C

Lisek, Anna; Orlikowska, T.

doi:10.1023/b:ticu.0000022551.96789.88

Cited by 25 publications

(14 citation statements)

References 23 publications

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“…Sodium alginate has been widely used in synthetic seed in various plant species. It has also been used to encapsulate plant organs for various purposes, such as improving a success rate of nodal segment propagation (West et al 2006), low temperature storage (Lisek and Olikowska 2004), desiccation (Janick et al 1989), bud propagation (Pattnaik and Chand 2000), and shoot tip propagation (Singh et al 2006). This study shows that alginate solution could be used to develop synthetic seeds by encapsulating S. alterniflora SEs or MPs derived from suspension cells.…”

Section: Discussionmentioning

confidence: 99%

Synthetic seed as a potential direct delivery system of mass produced somatic embryos in the coastal marsh plant smooth cordgrass (Spartina alterniflora)

Utomo

Wenefrida

Meche

et al. 2008

Plant Cell Tiss Organ Cult

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A suspension culture capable of producing a high quantity of proembryogenic masses (PEMs) was evaluated to provide adequate support for synthetic seed production in saltmarsh species Spartina alterniflora. Using immature inflorescences, callus was induced on MS medium supplemented with 2 mg 1 -1 2,4-dichlorophenoxyacetic acid (2,4-D). Prior to initiation of suspension culture, calli were proliferated on a modified R4 medium (R4+) for 2-4 weeks. Suspension culture was carried out in a liquid General Medium modified by adding FeSO 4 Á 7H 2 0, myoinositol, thiamine Á HCl, pyridoxine Á HCl, and casein acid hydrolysate. Suspension cells were subcultured weekly by replacing the spent medium with fresh medium. The amount of PEMs tripled in 1 week. Cells from suspension culture had an average regeneration rate of 90% for 6 months of culture. About 40 g of PEMs can be harvested from 1 l of suspension culture in a weekly basis. Plated on R4+ medium, these PEMs produced somatic embryos (SEs) that gave rise to 2,000 plantlets. Encapsulation of SEs or microplantlets (MPs) derived from suspension cells may facilitate direct delivery of micro-propagated S. alterniflora to field planting. Evaluated under in vitro conditions, both SEs and MP-derived synthetic seeds had high conversion rates.

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Section: Discussionmentioning

confidence: 99%

Synthetic seed as a potential direct delivery system of mass produced somatic embryos in the coastal marsh plant smooth cordgrass (Spartina alterniflora)

Utomo

Wenefrida

Meche

et al. 2008

Plant Cell Tiss Organ Cult

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show abstract

“…Although there has been steady research in the area of synthetic seed production, adequate attention has not been directed to storage of encapsulated propagules (Fujii et al, 1992). Storage of somatic embryos or vegetative propagules using an alginate-encapsulation protocol has been attempted in only a few species with various degrees of success (Piccioni and Standardi, 1995;Malabadi and Nataraja, 2002a, b;Sparg et al, 2002;Lisek and Orlikowska, 2003;Nieves et al, 2003).…”

Section: Introductionmentioning

confidence: 96%

Storability and germination of sodium alginate encapsulated somatic embryos derived from the vegetative shoot apices of mature Pinus patula trees

Malabadi

Staden

2005

Plant Cell Tiss Organ Cult

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Storability and germination of sodium alginate encapsulated somatic embryos derived from vegetative shoot apices of mature Pinus patula trees were tested on half strength DCR basal medium without growth regulators. The germination percentage of encapsulated somatic embryos was affected significantly by the concentration of sodium alginate and the duration of exposure to calcium chloride. Somatic embryos encapsulated with 2.5% sodium alginate dissolved in DCR basal salts gave significantly higher germination (89%) than other treatments. Short (5 min) incubation of the alginate encapsulated embryos in calcium chloride solution proved to be the best encapsulation procedure and the embryos subsequently gave the highest germination (89%). Synthetic seeds could be stored at 2°C for 120 days without a reduction in germination as opposed to non-encapsulated somatic embryos which showed only 9% germination after 20 days at 2°C. Germinated synthetic seeds produced normal plantlets. This study reports for the first time the storability of encapsulated somatic embryos generated from vegetative shoot apices of mature Pinus patula trees. This has potential for application in forestry.

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“…A synthetic seed is a somatic embryo or any non-embryogenic vegetative propagule (shoot tip, shoot primordial, axillary bud, nodal segment) inside an alginate hydrogel coating, possessing the ability to convert to a plant in vitro or ex vitro, an ability it can retain after storage. The use of unipolar propagules for development of synthetic seeds has been reported in numerous medical plant species, such as Valeriana wallichii (Mathur et al, 1989), Rauvolfia serpentina (Ray and Bhattacharya, 2008), Cineraria maritima (Srivastava et al, 2009), Cannabis sativa (Lata et al, 2009), Fragaria ananasa and Rubus idaeus (Lisek and Orlikowska, 2004). This kind of explant is very useful in such plant species, where somatic embryogenesis is not well established or else good quality somatic embryos are not produced.…”

Section: Introductionmentioning

confidence: 99%

A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

Grzegorczyk¹,

Wysokińska²

2011

Acta Biologica Cracoviensia Series Botanica

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Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3-acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequency of shoot and root emergence from encapsulated shoot tips was affected by the concentrations of sodium alginate and additives in the gel matrix (sucrose, gibberellic acid, MS nutrient medium) as well as duration of storage. The frequency of shoot and root induction of non-stored synthetic seeds was highest with shoot tips encapsulated with 2% sodium alginate containing 1.5% sucrose and 0.5 mg/l gibberellic acid (GA 3 ). Shoot tips maintained their viability and ability to develop shoots even after 24 weeks of storage when they were encapsulated in 3% alginate with 1/3 MS medium, sucrose (1.5%) and GA 3 (0.25 mg/l). Root formation tended to decrease with storage time. Overall, 90% of the plantlets derived from stored and non-stored synthetic seeds survived in the greenhouse and grew to phenotypically normal plants. This procedure can enable the use of synthetic seed technology for germplasm conservation of S. officinalis, a plant species of high medical and commercial value.K Ke ey y w wo or rd ds s: : Calcium alginate, cold storage, Salvia officinalis L., shoot tip encapsulation, synthetic seeds.

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In vitro Storage of Strawberry and Raspberry in Calcium-Alginate Beads at 4°C

Cited by 25 publications

References 23 publications

Synthetic seed as a potential direct delivery system of mass produced somatic embryos in the coastal marsh plant smooth cordgrass (Spartina alterniflora)

Synthetic seed as a potential direct delivery system of mass produced somatic embryos in the coastal marsh plant smooth cordgrass (Spartina alterniflora)

Storability and germination of sodium alginate encapsulated somatic embryos derived from the vegetative shoot apices of mature Pinus patula trees

A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

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