In vitro -studies in Plumbago zeylanica : rapid micropropagation and establishment of higher plumbagin yielding hairy root cultures

Verma, Praveen Chandra; Singh, Digvijay; Rahman, Laiq ur; Gupta, Madan M.; Banerjee, Sayan

doi:10.1078/0176-1617-00518

Cited by 65 publications

(34 citation statements)

References 14 publications

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“…Sahoo and Debata (1998) reported that a rapid and highly effective method for plant micropropagation from vegetative shoot buds was established for this medicinal plant Plumbago zeylanica L. ; multiple shoots were proliferated from nodal explants culture on MS basal medium supplemented with 0.25-1.0 mg/l Kn or 0.25-1.0 mg/l BAP. A similar phenomenon was observed in Plumbago zeylanica L. by other researchers (Gbadamosi and Egunyomi, 2010;Mallikadevi et al, 2008;Selvakumar et al, 2001;Rout et al, 1999;Verma et al, 2002).…”

supporting

confidence: 64%

In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

Hassan

Haque

Jahan

et al. 2012

Bangladesh J. Sci. Ind. Res.

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An efficient protocol was developed for in vitro mass propagation of an important medicinal shrub, Plumbago zeylanica L., (Plumbaginaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 86.4% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooted culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%.

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supporting

confidence: 64%

In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

Hassan

Haque

Jahan

et al. 2012

Bangladesh J. Sci. Ind. Res.

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“…1d) when cultured individually on root induction medium consisted of halfstrength MS medium with 0.5 mg/l IAA (Table II). Use of auxins singly or in combination for rooting was also reported by different authors (Gbadamosi and Egunyomi, 2010;Mallikadevi et al, 2008;Hassan and Khatun, 2010;Selvakumar et al, 2001;Rout et al, 1999;Verma et al, 2002).…”

Section: Resultsmentioning

confidence: 93%

In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

Haque

Hassan²,

Jahan

et al. 2012

Bangladesh J. Sci. Ind. Res.

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A protocol was established for in vitro shoot proliferation and plant regeneration of a rare medicinal shrub of Bangladesh, Plumbago indica L. (Plumbaginaceae) using shoot tip and nodal explants. Best shoot induction was observed on MS medium supplemented with 0.5 mg/l BAP, in which 92% of nodal explants responded to produce maximum number (42.8 ± 1.18) of shoots per explants. In vitro raised shoots rooted on half strength of MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting them in pots containing soil where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%.

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“…In other species of the genus Plumbago, such as P. rosea, the literature data also point to the accumulation of plumbagin in roots (Panichayupakaranant and Tewtrakul 2002). A study carried out by Verma et al 2002, revealed the potentialities of the hairy root cultures of P. zeylanica for the production of plumbagin. However, it is important to point out the work of Kitanov and Pashankov (1994), which showed that the flowers of the inflorescences of P. europaea have high concentrations of this substance.…”

Section: Resultsmentioning

confidence: 99%

Chemical composition fluctuations in roots of Plumbago scandens L. in relation to floral development

Paiva

Lima

Figueiredo

et al. 2011

An. Acad. Bras. Ciênc.

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Plumbago scandens L. is a Brazilian tropical/subtropical species that occurs along the coast. Chemically it is mainly represented by naphthoquinones, flavonoids, terpenoids and steroids. The aim of the present work is to study quantitative changes in the root metabolic production of Plumbago scandens during different physiologic developmental stages relative to floration. The results indicated the presence of four substances in the extracts: plumbagin, epi-isoshinanolone, palmitic acid and sitosterol, independent on developmental stage. The naphthoquinone plumbagin has always showed to be the major component of all extracts. Naphthoquinones exhibited their highest content during floration, while the content of the two others components decreased during this stage, revealing an inverse profile. The chemical composition changed depending on the plant requirements.

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In vitro -studies in Plumbago zeylanica : rapid micropropagation and establishment of higher plumbagin yielding hairy root cultures

Cited by 65 publications

References 14 publications

In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

Chemical composition fluctuations in roots of Plumbago scandens L. in relation to floral development

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