2022
DOI: 10.1038/s42003-022-04230-x
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In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

Abstract: The protein networks of cell-wall-biosynthesis assemblies are largely unknown. A key class of enzymes in these assemblies is the lytic transglycosylases (LTs), of which eleven exist in P. aeruginosa. We have undertaken a pulldown strategy in conjunction with mass-spectrometry-based proteomics to identify the putative binding partners for the eleven LTs of P. aeruginosa. A total of 71 putative binding partners were identified for the eleven LTs. A systematic assessment of the binding partners of the rare lipopr… Show more

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Cited by 9 publications
(40 citation statements)
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“…Category 3 (lower gray highlighted entries in Figure 6) are the new binding partners identified only by RlpA‐ 2 photo‐chemical enrichment. The documentation of in vitro interaction of the proteins of the first two categories with RlpA has been reported previously by the pull‐down strategy (Avila‐Cobian et al, 2022). The additional protein partners to RlpA in the third category are characterized in this study.…”
Section: Resultsmentioning
confidence: 60%
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“…Category 3 (lower gray highlighted entries in Figure 6) are the new binding partners identified only by RlpA‐ 2 photo‐chemical enrichment. The documentation of in vitro interaction of the proteins of the first two categories with RlpA has been reported previously by the pull‐down strategy (Avila‐Cobian et al, 2022). The additional protein partners to RlpA in the third category are characterized in this study.…”
Section: Resultsmentioning
confidence: 60%
“…Figure 6 displays the putative‐protein‐binding partners identified from all enriched protein samples, which are organized into three categories. Category 1 (upper unhighlighted entries in Figure 6) shows binding partners identified by the RlpA‐ 2 and RlpA‐WT analyses and by our previous pulldown analyses (Avila‐Cobian et al, 2022). Category 2 (blue highlighted entries in Figure 6) are partners identified with RlpA‐WT and by our previous pulldown analyses (Avila‐Cobian et al, 2022).…”
Section: Resultsmentioning
confidence: 90%
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