In Vitro studies on changes in selected biochemical parameters and morphology of Sertoli cells in mice overexpressing bovine growth hormone

Abstract

-The aim of the present study was to investigate the changes in selected biochemical variables in

In vitro studies on the effect of 8-(4-chlorophenylthio) cAMP on selected biochemical parameters in Sertoli cells isolated from testis of transgenic mice

“…Total proteins, lactic acid, DNA and RNA from normal and transgenic Sertoli Cells were determined by methods described previously [13][14][15]. All the biochemical parameters were performed in isolated Sertoli cells from normal and transgenic mice treated with or without cAMP analog and moreover, the results were compared to our earlier findings [6], in order to assess the interactive effect of cAMP analog and GH in the regulation of metabolic fate of Sertoli cells isolated from testis of both transgenic and normal mice.…”

“…After completion of two days, the dishes were shaken gently and fresh incubation medium was replaced. The percent spreading, maintenance capacity and culture characteristics was determined on day 4 of culture was performed according to Bhaskar [6]. Total proteins, lactic acid, DNA and RNA from normal and transgenic Sertoli Cells were determined by methods described previously [13][14][15].…”

“…Thus, a cross-talk between gonadotropins such as follicle stimulating hormone (FSH), leutinizing hromone (LH), testosterone and GH might be responsible for accelerated spermatogenic effects [1][2][3][4]. Furthermore, some preliminary data in vitro suggested that GH positively modulates Sertoli cell DNA synthesis, targets machinery of transcription and translation to trigger protein synthesis and also provides adequate levels of energy through lactate for sertoli cells thereby promote spermatogenesis [5,6]. Thus, it is apparent that GH has ability to modulate the metabolic fate of sertoli cells and influence spermatogenesis.…”