In Vitro Studies on Pesticide-Induced Oxidative DNA Damage

Abstract

Pesticides are among the most extensively used chemicals in the world today and they are also among the most hazardous compounds for the human. This study was designed to use the plasmid relaxation assay to describe the association of markers of DNA damage with pesticide exposure. The DNA damage activity of fluoxastrobin and imazamox were checked on pBluescript M13+ plasmid DNA (3.2 kb) in the absence and presence of Cu(II) ions. It has been found that the fluoxastrobin and imazamox can cleavage plasmid DNA in the absence and presence of Cu(II) ions. DNA cleavage was found to be concentration-and time-dependent. In conclusion, the present study showed that fluoxastrobin and imazamox can damage DNA, which warrants for further investigations to correctly evaluate the hazards of exposure to these chemicals.