In vitro studies on reductive vinyl chloride dehalogenation by an anaerobic mixed culture

Rosner, Bettina; McCarty, Perry L.; Spormann, Alfred M.

doi:10.1128/aem.63.11.4139-4144.1997

Cited by 69 publications

(34 citation statements)

References 53 publications

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“…Previous work has demonstrated that the mixed culture used in the present study contains at least two populations of reductive dechlorinating microorganisms to achieve complete dechlorination [29]. The need for multiple dechlorinating strains to completely reduce PCE to ETH has been observed in other studies as well [45], and apparently, the Steol CS-330 had a strong influence at these high concentrations on the DCE-dechlorinating organism(s). Interestingly, Steol CS-330 and other surfactants also influenced methane production in the microcosms studies, because lower levels of methane were observed in the surfactant-added microcosms compared to the surfactant-free controls.…”

Section: Effect Of Surfactant Concentration On Pce Dechlorination Actsupporting

confidence: 60%

Effects of surfactants on the dechlorination of chlorinated ethenes

McGuire¹,

Hughes

2003

Enviro Toxic and Chemistry

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The influence of surfactants on a perchloroethene (PCE) dechlorinating mixed culture was investigated in laboratory experiments. Surfactants (Steol CS-330, Aerosol MA 80-I, alpha olefin sulfonate 14 to 16, Neodol 25-7, Tween 80, alkyl polyglycoside, C16TAB [trimethylammonium bromide], and sodium dodecyl sulfate) were evaluated for their effects on the rate and extent of PCE reductive dechlorination and their potential biodegradation by the mixed culture. Limited, if any, surfactant biodegradation was observed for the surfactants tested, and all surfactants impaired dechlorination in either the rate of PCE dechlorination or the terminal dechlorination products observed. Based on initial testing, a nonionic surfactant, Tween 80, and an anionic surfactant, Steol CS-330, were selected for additional investigation. Dechlorination of PCE to dichloroethene (DCE), vinyl chloride (VC), and ethene (ETH) occurred in all Tween 80-amended microcosms, with a depressed rate of ETH production as the only adverse effect. Steol CS-330, however, inhibited dechlorination beyond DCE at all surfactant concentrations exceeding 25 mg/L. Attempts to acclimate a culture to Steol CS-330 were unsuccessful. Inhibition of VC and ETH production was reversible on dilution of the surfactant to a concentration of 10 mg/L or less, indicating that surfactant interactions with the enzyme system responsible for reductive dechlorination of DCE may be the cause of inhibition.

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Section: Effect Of Surfactant Concentration On Pce Dechlorination Actsupporting

confidence: 60%

Effects of surfactants on the dechlorination of chlorinated ethenes

McGuire¹,

Hughes

2003

Enviro Toxic and Chemistry

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“…Bagley (1998) developed a general growth model for the case where one species was responsible for both PCE and TCE dechlorination, and another species for cDCE and VC dehalogenation. Our previous studies have indicated for our culture that dechlorination of PCE to ethene involves two separate species, one that converts PCE and TCE to cDCE, and the other, cDCE and VC to ethene Rosner, et al, 1997;Sharma and McCarty, 1996). Ballapragada et al (Ballapragada et al, 1997) reported the same for their mixed culture.…”

Section: Resultssupporting

confidence: 59%

Mechanisms, Chemistry, and Kinetics of Anaerobic Biodegradation of cis-Dichloroethene and Vinyl Chloride

McCarty¹,

Spormann²

2000

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“…Under aerobic conditions, PCE is considered nonbiodegradable, while TCE can be broken down to mainly nontoxic products by certain nonspecific oxygenases, such as methane monooxygenase or toluene dioxygenase (5,14). Under anaerobic conditions, PCE and TCE have been reductively dechlorinated by mixed cultures to less-chlorinated ethenes (30) and, under certain conditions, to the nontoxic products ethene (ETH) (4,10,19,26) and ethane (2).…”

mentioning

confidence: 99%

Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by “ Dehalococcoides ethenogenes ” 195

Maymó-Gatell

Anguish

Zinder

1999

Appl Environ Microbiol

319

140

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“Dehalococcoides ethenogenes” 195 can reductively dechlorinate tetrachloroethene (PCE) completely to ethene (ETH). When PCE-grown strain 195 was transferred (2% [vol/vol] inoculum) into growth medium amended with trichloroethene (TCE),cis-dichloroethene (DCE), 1,1-DCE, or 1,2-dichloroethane (DCA) as an electron acceptor, these chlorinated compounds were consumed at increasing rates over time, which indicated that growth occurred. Moreover, the number of cells increased when TCE, 1,1-DCE, or DCA was present. PCE, TCE, 1,1-DCE, and cis-DCE were converted mainly to vinyl chloride (VC) and then to ETH, while DCA was converted to ca. 99% ETH and 1% VC. cis-DCE was used at lower rates than PCE, TCE, 1,1-DCE, or DCA was used. When PCE-grown cultures were transferred to media containing VC ortrans-DCE, products accumulated slowly, and there was no increase in the rate, which indicated that these two compounds did not support growth. When the intermediates in PCE dechlorination by strain 195 were monitored, TCE was detected first, followed bycis-DCE. After a lag, VC, 1,1-DCE, andtrans-DCE accumulated, which is consistent with the hypothesis that cis-DCE is the precursor of these compounds. Both cis-DCE and 1,1-DCE were eventually consumed, and both of these compounds could be considered intermediates in PCE dechlorination, whereas the small amount oftrans-DCE that was produced persisted. Cultures grown on TCE, 1,1-DCE, or DCA could immediately dechlorinate PCE, which indicated that PCE reductive dehalogenase activity was constitutive when these electron acceptors were used.

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In vitro studies on reductive vinyl chloride dehalogenation by an anaerobic mixed culture

Cited by 69 publications

References 53 publications

Effects of surfactants on the dechlorination of chlorinated ethenes

Effects of surfactants on the dechlorination of chlorinated ethenes

Mechanisms, Chemistry, and Kinetics of Anaerobic Biodegradation of cis-Dichloroethene and Vinyl Chloride

Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by “ Dehalococcoides ethenogenes ” 195

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