In vitro study of an ethanolic extract of coffea leaves to inhibit freshwater pathogenic bacteria

Kenconojati, Hapsari; Ulkhaq, Mohammad Faizal; Budi, Darmawan Setia; Azhar, Muhammad Hanif

doi:10.1088/1755-1315/236/1/012082

Cited by 3 publications

(6 citation statements)

References 19 publications

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“…Recently, industry applications of coffee leaves such as therapeutic agents for hypertension, gastrointestinal inflammation, and diabetes; tobacco substitute; animal feed; packaging material; personal hygienic products; vehicle perfume; and antimicrobial agents et al. have been discovered (Chen, 2019; Kenconojati, Ulkhaq, Budi, & Azhar, 2019; Segheto et al., 2018). Due to the rich resources and various applications, extraction of bioactive components from coffee leaves is considered a promising way to produce value‐added products such as functional food, natural health products, and beverage.…”

Section: Introductionmentioning

confidence: 99%

Optimization of ultrasonic‐assisted extraction conditions for bioactive components from coffee leaves using the Taguchi design and response surface methodology

Chen

Ding

et al. 2020

Journal of Food Science

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Coffee leaves contain various bioactive compounds that are beneficial for human health. However, there are very limited researches related to the extraction of the bioactive phytochemicals from coffee leaves. In the present study, the extraction conditions for bioactive components from coffee leaves were optimized using Taguchi design and response surface methodology (RSM). Taguchi design was used to screen significant factors that affected the yield of phytochemicals including trigonelline, caffeine, chlorogenic acids, mangiferin, and rutin, total phenolic content (TPC) and antioxidant activity. Sequentially, a Box‐Behnken design (BBD) was used to optimize the extraction conditions. Three factors including Liquid‐to‐solid (L:S) ratio, ethanol concentration, and extraction temperature that significantly affected most of the phytochemical yields and antioxidant activity were selected from the six variables using Taguchi design. The optimal extraction conditions obtained from RSM were 30.3:1 L:S ratio, 54.5% ethanol, and 80 °C when simultaneously considered four responses, including TPC, the yields of mangiferin and 5‐CQA and DPPH scavenging capacity. Under the optimal conditions, the experimental results for the above four responses were 62.1 mg gallic acid/g, 4.1 mg/g, 11.4 mg/g, and 356.9 µmol Trolox/g, respectively, which were close to the predicted values. About 97% of phytochemicals can be extracted in the first two times of extraction. In conclusion, the combination of Taguchi design and response surface methodology can be successfully used to screen and optimize the significant factors that affected the bioactive components extracted from coffee leaves. Practical Application Coffee leaves, the byproducts of coffee plants, are considered no‐ or low‐value although it has a long history for using them as tea‐like beverage and ethnomedicine by locals in the coffee plant growing countries. Bioactive components extracted from coffee leaves can be used as ingredients in functional beverages, functional food, and natural health products. These applications will add values to coffee leaves as well as increase the incomes of coffee farmers and workers.

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Section: Introductionmentioning

confidence: 99%

Optimization of ultrasonic‐assisted extraction conditions for bioactive components from coffee leaves using the Taguchi design and response surface methodology

Chen

Ding

et al. 2020

Journal of Food Science

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“…in which coffee ( Coffea canephora ) leaf ethyl acetate extract was more effective against Edwardsiella tarda (Gram‐negative) compared to Streptococcus agalactiae (Gram‐positive), which suggests that the coffee leaves extract is more effective against Gram‐negative bacteria. The same authors also revealed best results of ethanol extract of coffee leaves against Gram‐negative bacteria ( Aeromonas hydrophila ) in comparison with Gram‐positive bacteria ( Streptococcus agalactiae ) that affect freshwater fish 26 …”

Section: Resultsmentioning

confidence: 83%

“…The same authors also revealed best results of ethanol extract of coffee leaves against Gram-negative bacteria (Aeromonas hydrophila) in comparison with Gram-positive bacteria (Streptococcus agalactiae) that affect freshwater fish. 26 In tests with Pseudomonas aeruginosa (ATCC 27853 and ATCC 9027), the MIC for both strains was higher than 5000 μg/mL, which demonstrated the ineffectiveness of the extract against these microorganisms at tested concentrations. The ineffectiveness of the extract against Gram-negative Pseudomonas aeruginosa (ATCC 27853 and ATCC 9027) may be related to the natural resistance of this strain against many drugs classes.…”

Section: In Vitro Antibacterial Activitymentioning

confidence: 92%

“…Leaves were dried at 40.0°C under forced air circulation (SL‐102; SOLAB, Piracicaba, Brazil), and then ground using a blender (METVISA LAR 2; BIMG Brasil, Brusque, Brazil) and knife mill (SL 30; SOLAB). EE‐CaL was prepared as described by Kenconojati et al 26 . with modifications.…”

Section: Methodsmentioning

confidence: 99%

“…Leaves were dried at 40.0 C under forced air circulation (SL-102; SOLAB, Piracicaba, Brazil), and then ground using a blender (METVISA LAR 2; BIMG Brasil, Brusque, Brazil) and knife mill (SL 30; SOLAB). EE-CaL was prepared as described by Kenconojati et al 26 with modifications. The coffee leaf powder (300 g) was macerated with 1.2 L of ethanol for 24 h, at 25 C, under stirring (120 rpm) in a shaker incubator (SL-223, SOLAB).…”

Section: Preparation Of the Ethanol Extractmentioning

confidence: 99%

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Chemical characterisation by UPLC‐Q‐ToF‐MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by‐product

Júnior

Costa

Mello

et al. 2022

Phytochemical Analysis

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Introduction: Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.Objectives: This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).Material And Methods: The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS). The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively. Results:The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect). Conclusion:The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties. Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.

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Analisis Indeks Keanekaragaman, Keragaman, dan Dominansi Ikan di Sungai Aur Lemau Kabupaten Bengkulu Tengah

Febrian¹,

Nursa’adah²,

Karyadi³

2022

Bioscientist j. ilm. bosbiol.

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Fish is one of the biological natural resources that has high diversity in river ecosystems. The purpose of this study was to determine the diversity index, diversity index, and fish dominance index in the Aur Lemau Watershed, which is located in Pondok Kelapa District, Central Bengkulu Regency. This type of research is descriptive quantitative. This research was conducted in January-August 2021. The sampling method used was purposive random sampling. Determination of the sampling location is recommended in the relatively different environmental conditions and fishing areas that are usually carried out by the surrounding community. This study found as many as 14 species of fish with a total number of 146 fish. The order that dominates the fish in the waters of the Aur Lemau River is the order Perciformes with a total of 8 families and 8 species. Based on the results of the calculation of the diversity index value of station I is 2.09; station II of 1.9; and station III of 1.3. Then, the uniformity index value of station I (0.96); station II (0.92); and station III (0.81). The highest dominance index is at station III with a value of 0.31. The dominance index value of station I is 0.14 and station II is 0.12.

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In vitro study of an ethanolic extract of coffea leaves to inhibit freshwater pathogenic bacteria

Cited by 3 publications

References 19 publications

Optimization of ultrasonic‐assisted extraction conditions for bioactive components from coffee leaves using the Taguchi design and response surface methodology

Optimization of ultrasonic‐assisted extraction conditions for bioactive components from coffee leaves using the Taguchi design and response surface methodology

Chemical characterisation by UPLC‐Q‐ToF‐MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by‐product

Analisis Indeks Keanekaragaman, Keragaman, dan Dominansi Ikan di Sungai Aur Lemau Kabupaten Bengkulu Tengah

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