In vitro study of cartilage tissue engineering using human adipose-derived stem cells induced by platelet-rich plasma and cultured on silk fibroin scaffold

Rosadi, Imam; Karina, Karina; Rosliana, Iis; Sobariah, Siti; Afini, Irsyah; Widyastuti, Tias; Barlian, Anggraini

doi:10.1186/s13287-019-1443-2

Cited by 46 publications

(46 citation statements)

References 55 publications

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“…The effect of PRP on MSC chondrogenesis has been reported in several studies. Addition of PRP 10% into differentiation medium was reported to be the most optimal concentration to induce chondrogenesis in hADSC (human adipose-derived stem cells) 16 , 53 , 54 by increasing the synthesis of GAG and collagen type II into the surrounding ECM after 21 days. Activated PRP releases several growth factors, with TGF-β1 (transforming growth factor beta) being one of the highest in concentration 55 , 56 .…”

Section: Resultsmentioning

confidence: 99%

“…TGF-β1 binds to TGF receptor, causing signaling cascade involving Smad 2/3 and 4 protein which then translocate into the nucleus and interacting with transcription factor Sox 9. Sox 9 is important in regulating expression of collagen type II and GAG 54 , 57 .…”

Section: Resultsmentioning

confidence: 99%

“…TGF-β1 binds to TGF receptor, causing signaling www.nature.com/scientificreports/ cascade involving Smad 2/3 and 4 protein which then translocate into the nucleus and interacting with transcription factor Sox 9. Sox 9 is important in regulating expression of collagen type II and GAG 54,57 . Overall, in this research scaffold with silk spidroin (SF 90% + SS 10%) was able to support chondrogenic differentiation of hWJ-MSCs significantly better in comparison to silk fibroin scaffold (SF 100%) as shown in collagen type II ICC and GAG accumulation.…”

Section: Effect Of Laa or Prp Supplementation On Hwj-msc Proliferationmentioning

confidence: 99%

“…The cells were incubated with secondary antibody (Alexa Fluor 488, Abcam) for 2 h. 2,5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) was added to stain cell nucleus. The images were observed using a confocal microscope (Olympus Fv1200) at three different points 54 .…”

Section: Optimization Of Prp and Laa Concentration As Chondrogenesis mentioning

confidence: 99%

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Chondrogenic differentiation of Wharton’s Jelly mesenchymal stem cells on silk spidroin-fibroin mix scaffold supplemented with L-ascorbic acid and platelet rich plasma

Barlian

Judawisastra

Ridwan

et al. 2020

Sci Rep

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In this research, hWJ-MSCs were grown on silk scaffolds and induced towards chondrogenesis by supplementation with L-ascorbic acid (LAA) or platelet rich plasma (PRP). Silk scaffolds were fabricated with salt leaching method by mixing silk fibroin (SF) with silk spidroin (SS). The silk fibroin was obtained from Bombyx mori cocoon that had been degummed, and the silk spidroin was obtained from wild-type spider Argiope appensa. The effect of scaffold composition and inducer on cell proliferation was observed through MTT assay. The most optimal treatment then continued to be used to induce hWJ-MSC towards chondrogenic differentiation for 7 and 21 days. Scaffolds characterization showed that the scaffolds produced had 3D structure with interconnected pores, and all were biocompatible with hWJ-MSCs. Scaffold with the addition of 10% SS + 90% SF showed higher compressive strength and better pore interconnectivity in comparison to 100% silk fibroin scaffold. After 48 h, cells seeded on scaffold with spidroin and fibroin mix had flattened morphology in comparison to silk fibroin scaffold which appeared to be more rounded on the scaffold surface. Scaffold with 10% (w/w) of silk spidroin (SS) + 90% (w/w) of silk fibroin (SF) was the most optimal composition for cell proliferation. Immunocytochemistry of integrin β1 and RGD sequence, showed that scaffold with SS 10% provide better cell attachment with the presence of RGD sequence from the spidroin silk which could explain the higher cell proliferation than SF100% scaffold. Based on Alcian Blue staining and Collagen Type II immunocytochemistry (ICC), cells grown on 10% SS + 90% SF scaffold with 10% PRP supplementation were the most optimal to support chondrogenesis of hWJ-MSCs. These results showed that the addition of spidroin silk from A. appensa. had impact on scaffold compressive strength and chondrogenic differentiation of hWJ-MSC and had the potential for further development of bio-based material scaffold in cartilage tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Effect Of Laa or Prp Supplementation On Hwj-msc Proliferationmentioning

confidence: 99%

Section: Optimization Of Prp and Laa Concentration As Chondrogenesis mentioning

confidence: 99%

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Chondrogenic differentiation of Wharton’s Jelly mesenchymal stem cells on silk spidroin-fibroin mix scaffold supplemented with L-ascorbic acid and platelet rich plasma

Barlian

Judawisastra

Ridwan

et al. 2020

Sci Rep

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“…el punca adalah sel yang memiliki kemampuan memperbarui diri dan berdiferensiasi menjadi berbagai jenis sel [1,2]. Dengan kemampuan tersebut, sel punca memainkan peran penting dalam regenerasi jaringan untuk penyakit degeneratif [3,4].…”

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Pengaruh Penambahan Berbagai Konsentrasi L-Ascorbic Acid Terhadap Kemampuan Migrasi Adipose-Derived Stem Cells Asal Manusia

Karina¹,

Rosadi²,

Subroto³

et al. 2020

SST

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Abstrak - Mesenchymal Stem Cells (MSCs) merupakan sel yang memiliki kemampuan memperbarui diri dan berdiferensiasi menjadi berbagai jenis sel. MSCs berperan penting dalam perbaikan dan regenerasi jaringan. Adipose-Derived Stem Cells (ADSCs) merupakan MSCs yang didapatkan dari jaringan lemak. ADSCs telah banyak digunakan dalam studi MSCs karena ketersediaan sumber sel yang banyak dalam jaringan tubuh dan proses isolasinya lebih mudah serta memiliki risiko yang lebih rendah. Pada studi ini, ADSCs digunakan untuk mengetahui kemampuan migrasi dalam medium yang mengandung fetal bovine serum 10% (FBS) dengan penambahan L-Ascorbic Acid (LAA) konsentrasi 0%, 1%, 3% dan 6%. Kuantifikasi kemampuan migrasi dilakukan dengan menggunakan teknik goresan luka. ADSCs yang dikulturkan menunjukkan morfologi seperti fibroblas. Luas penutupan luka menunjukkan hasil tertinggi dengan perbedaan yang tidak signifikan pada penambahan konsentrasi LAA 3% dan 6% dibandingkan penambahan LAA 0%, 1% dan kontrol. Hasil ini juga ditunjukkan pada pasase 7, 8, dan 9. ADSCs menunjukkan kemampuan migrasi yang optimal pada penambahan LAA 3% dan 6% dibandingkan medium dengan penambahan LAA 0%, 1% dan kontrol.Abstract - Mesenchymal stem cells (MSCs) are multilineage cells with the ability to self-renew and differentiate into a variety of cell types. MSCs play important role in tissue repair and regeneration. Adipose-Derived Stem Cells (ADSCs) are MSCs derived from fat tissue. ADSCs has been widely used in MSCs studies because it require more cell sources in body tissue and the process of isolation is easier and has lower risk. In this study, ADSCs were used to determine migration ability in a medium containing 10% bovine fetal serum (FBS) using 0%, 1%, 3% and 6% of L-Ascorbic Acid (LAA) concentrations. Quantification of migration ability was done by using scratch technique. ADSCs were cultured display fibroblasts-like morphology. The closure of wound area is highest with not significantly difference in LAA concentrations of 3% and 6% compared to LAA 0%, 1% and control. This results were also showed on 7, 8, and 9 cell passage. ADSCs showed optimal migration ability at LAA 3% and 6% compared to media with LAA 0%, 1% and control.Keywords - ADSCs, Cells Migration, LAA.

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Biomimetic Structure Hydrogel Loaded with Long‐Term Storage Platelet‐Rich Plasma in Diabetic Wound Repair

Ding,

Fu,

Gui

et al. 2023

Adv Healthcare Materials

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Exploring the preparation of multifunctional hydrogels from a bionic perspective is an appealing strategy. Here, a multifunctional hydrogel dressing inspired by the characteristics of porous extracellular matrix produced during Acomys wound healing is prepared. These dressings are printed by digital light processing printing of hydrogels composed of gelatin methacrylate, hyaluronic acid methacrylate, and pretreated platelet‐rich plasma (PRP) to shape out triply periodic minimal surface structures, which are freeze‐dried for long‐term storage. These dressings mimic the porous extracellular matrix of Acomys, while the freeze‐drying technique effectively extends the storage duration of PRP viability. Through in vivo and in vitro experiments, the biomimetic dressings developed in this study modulate cell behavior and facilitate wound healing. Consequently, this research offers a novel approach for the advancement of regenerative wound dressings.

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In vitro study of cartilage tissue engineering using human adipose-derived stem cells induced by platelet-rich plasma and cultured on silk fibroin scaffold

Cited by 46 publications

References 55 publications

Chondrogenic differentiation of Wharton’s Jelly mesenchymal stem cells on silk spidroin-fibroin mix scaffold supplemented with L-ascorbic acid and platelet rich plasma

Chondrogenic differentiation of Wharton’s Jelly mesenchymal stem cells on silk spidroin-fibroin mix scaffold supplemented with L-ascorbic acid and platelet rich plasma

Pengaruh Penambahan Berbagai Konsentrasi L-Ascorbic Acid Terhadap Kemampuan Migrasi Adipose-Derived Stem Cells Asal Manusia

Biomimetic Structure Hydrogel Loaded with Long‐Term Storage Platelet‐Rich Plasma in Diabetic Wound Repair

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