In Vitro Study of Concentration-Effect and Time-Course Pattern of White Alum on Escherichia Coli O157:h7 Growth

Shahriari, Reza; Salari, Saeed; Shahriari, Saeed

doi:10.21010/ajtcam.v14i2.32

Cited by 7 publications

(7 citation statements)

References 14 publications

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“…The effect of alum on gingival health, cytotoxicity and safety for oral human health had been earlier reported [9]. PA has demonstrated activity against oral microbiota.…”

Section: Periodontologymentioning

confidence: 89%

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A Review of Antimicrobial Properties of Alum and Sundry Applications

Amadi¹

2020

Act Scie Micro

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Potassium aluminium sulphate (PAS) commonly referred to as 'potash alum (PA)', 'alum' or 'tawas' has recently drawn the attention of the scientific community as an efficient, safe and eco-friendly inorganic compound, commercially available and cost effective.It demonstrated a high propensity of antimicrobial activity in a variety of systems traditionally and scientifically. Several In vitro and In vivo studies report that alum individually or in synergism have antimicrobial properties against a broad spectrum of bacterial and fungal species and harnesses other activities beneficial to humans. This article has become crucial especially at a time when the world is bedevilled with antibiotic drug abuse resulting in antimicrobial resistance (AMR). Alum, however, has found applications in a wide spectrum of human activities such as pharmaceutical, cosmetic, food, textile and synthetic industries. This review presents an update of findings of this inorganic sulphate salt as an antimicrobial agent and miscellaneous applications.

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“…The effect of alum on gingival health, cytotoxicity and safety for oral human health had been earlier reported [9]. PA has demonstrated activity against oral microbiota.…”

Section: Periodontologymentioning

confidence: 89%

“…Such effect was dose and time dependent as well as with other disinfectants. Strong antibacterial activity of white alum compared with control was shown against tested bacterium and could be used as an inhibitor of bacterial growth [9].…”

Section: Antibacterial Effect Of Alummentioning

confidence: 98%

A Review of Antimicrobial Properties of Alum and Sundry Applications

Amadi¹

2020

Act Scie Micro

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“…Moreover, PX markedly increased the levels of probiotic gut microbiota [ 41 ] such as the genus Alloprevotella and Prevotellaceae UCG 001. Based on KEGG analysis and RNA sequencing, PX exhibits a strong microbicidal effect by using AF, CR, AL, and TV, as well as white alunite, which is a natural product with a potential antivirus [ 42 ] and antibacterial capacity [ 43 ]. Compared with the 16S rRNA sequencing results of the PX formula, no significant alteration in microbial communities has been observed in the XH-treated CRC group, which might explain the poor efficacy of the XH formula.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Mechanism of Pingxiao Formula against Colorectal Cancer

Wang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Colorectal cancer (CRC) is the most common cancer worldwide and develops due to a broad range of causative factors. Pingxiao (PX) formula and Xihuang (XH) formula are two commonly used drugs to treat CRC, especially as an alternative therapy for those patients who could not suffer surgery, chemotherapy, or immunotherapy, namely, elder or advanced CRC patients. However, the pertinent pharmacological mechanisms are still elusive. The investigation was designed to explain the pharmacological mechanisms of the PX formula. A murine model of CRC was established by injecting CT26.WT cells into the caecum of 4-week-old male Balb/c mice, following PX or XH treatment for 30 days. Network pharmacology analysis combined with weighted gene coexpression network analysis (WGCNA) predicted the pharmacological mechanisms and therapeutic value. High-throughput 16S rRNA sequencing determined the alterations in the gut microbiota communities. Western blotting, immunofluorescence, and flow cytometry examined the influence of PX on the tumor microenvironment (TME). Injection of CT26.WT-induced CRC in Balb/c mice was markedly attenuated by PX treatment. Compared with XH administration, PX exhibited a stronger antitumor effect, such as smaller tumor volume, lower interleukin 17 (IL-17), IL-6 and tumor necrosis factor-alpha (TNFα) serum levels, and higher interferon-gamma (IFN-γ) concentration. Network pharmacology analysis demonstrated that both PX and XH targets were enriched in cancers and inflammatory responses. RNA sequencing confirmed that PX treatment induced cancer cell apoptosis and inhibited inflammatory reactions within the tumor. Moreover, the PX formula considerably restored homeostasis of the gut microbiota, which was not observed in the XH group. PX targets, those associated with the survival probability of CRC patients, correlated with macrophage (Mφ) infiltration, which presented an independent risk factor for the CRC outcome. PX treatment promoted the transition of alternatively activated Mφs (M2 Mφs) to classically activated Mφs (M1 Mφs). Moreover, the peritoneal Mφs from the PX group inhibited the migration of CW26.WT cells, as evidenced by the wound healing experiment and transwell assay, which was consistent with the decreased expression of the vascular endothelial growth factor (VEGF). Furthermore, the coculturing system confirmed that PX-treated Mφs suppressed colorectal tumor-derived organoid proliferation. PX formula exhibits a potential antitumor effect against CRC by suppressing the colonization of pathological microorganisms, reshaping Mφ effector functions and hence inhibiting cancer cell proliferation.

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“…In addition, baicalin has shown an effect on inhibiting growth and reducing antimicrobial resistance of Escherichia coli [14]. Alum contains aluminum sulfate and has been shown to possess good antibacterial activity [15]. It has been documented that a commercial product, Huangqinsulv capsule, of which the main active ingredient is baicalin-aluminium complex, was synthesized by using alum and the extracts of Scutellaria baicalensis Georgi [16].…”

Section: Introductionmentioning

confidence: 99%

Effect of Baicalin-Aluminum Complexes on Fecal Microbiome in Piglets

Zhuang

Li-wei

et al. 2019

IJMS

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The gut microbiome has important effects on gastrointestinal diseases. Diarrhea attenuation functions of baicalin (BA) is not clear. Baicalin–aluminum complexes (BBA) were synthesized from BA, but the BBA’s efficacy on the diarrhea of piglets and the gut microbiomes have not been explored and the mechanism remains unclear. This study has explored whether BBA could modulate the composition of the gut microbiomes of piglets during diarrhea. The results showed that the diarrhea rate reduced significantly after treatment with BBA. BBA altered the overall structure of the gut microbiomes. In addition, the Gene Ontology (GO) enrichment analysis indicated that the functional differentially expressed genes, which were involved in the top 30 GO enrichments, were associated with hydrogenase (acceptor) activity, nicotinamide-nucleotide adenylyltransferase activity, and isocitrate lyase activity, belong to the molecular function. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that flagellar assembly, bacterial chemotaxis, lipopolysaccharide biosynthesis, ATP-binding cassette transporters (ABC) transporters, biosynthesis of amino acids, and phosphotransferase system (PTS) were the most enriched during BBA treatment process. Taken together, our results first demonstrated that BBA treatment could modulate the gut microbiomes composition of piglets with diarrhea, which may provide new potential insights on the mechanisms of gut microbiomes associated underlying the antimicrobial efficacy of BBA.

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In Vitro Study of Concentration-Effect and Time-Course Pattern of White Alum on Escherichia Coli O157:h7 Growth

Cited by 7 publications

References 14 publications

A Review of Antimicrobial Properties of Alum and Sundry Applications

A Review of Antimicrobial Properties of Alum and Sundry Applications

Pharmacological Mechanism of Pingxiao Formula against Colorectal Cancer

Effect of Baicalin-Aluminum Complexes on Fecal Microbiome in Piglets

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