In-vitro Study of DNA-Synthesis Time and Cell-Cycle Time in Erythrocyte Precursors of Normal and Thalassaemic Subjects, Using a &lt;sup&gt;3&lt;/sup&gt;H- and &lt;sup&gt;14&lt;/sup&gt;C-Thymidine Double Labelling Technique

Kesse‐Elias, M.; Harriss, E. B.; Gyftaki, E.

doi:10.1159/000209013

Cited by 31 publications

(10 citation statements)

References 7 publications

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“…Special atten tion to the basophilic cells was given in an autoradiographic in vitro study using the double-labelling technique with 3H-and 14C-thymidine [2]. In this study a shortening of the DNA synthesis time from the normal average of 17.9 to 10.0 hand a shortening of the generation time from 23.0 to 13.4 h was observed in 9 cases of thalassaemia major.…”

Section: Discussionmentioning

confidence: 68%

Erythropoietic Cell Proliferation in Different Clinical States of β-Thalassaemia

Queißer¹,

Betzler²,

Heimpel³

et al. 1971

Acta Haematol

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Erythropoietic cell proliferation of 6 cases of different clinical states of β--thalassaemia (thal) was studied by a combined method using Feulgen-cytophotometry and autoradiography after in vitro labelling with ³H-TdR. An abnormal distribution of the DNA content and ³H-TdR incorporation was found, which was more pronounced in the one patient with thal major than in the 3 patients with thal minor and was not provable in the subclinical form (minima), indicating a direct relationship to the ineffective erythropoiesis and the states of anaemia in this disease. This proliferation disturbance consisted of an accumulation of cells in G₁, a decreased proportion of cells in S and a decreased S/G₂ ratio, and was limited to the early polychromatic cell compartment. In the basophilic erythroblasts, a decreased proportion of cells in G₁ was observed revealing a shortening of the G₁ period in this cell compartment

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Section: Discussionmentioning

confidence: 68%

Erythropoietic Cell Proliferation in Different Clinical States of β-Thalassaemia

Queißer¹,

Betzler²,

Heimpel³

et al. 1971

Acta Haematol

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“…In scven of the cases studied there was also an increased frequency of G, cells relative to the small number in DNA synthesis, suggesting a failure to enter mitosis.The death of these arrested cells could account for the ineffectiveness of erythropoiesis in this disease. Low serum folate levels were found in all the patients who were not already on folate therapy, but the disturbance in cell proliferation persisted after the administration of folic acid.Ferrokinctic and porphyrin excretion studies have shown that ineffective erythropoiesis is an important factor in the pathogenesis of the anaemia in P-thalassaemia (Sturgeon & Finch, 1957; Grinstein ct al, 1960;Malamos et al, 1961) and indicate that, in addition to the impairment of P-chain synthesis, there must also be a disorder of erythroblast proliferation in this disease. This paper describes the results of a study of erythroblast proliferation in P-thalassaemia, using a combination of quantitative cytochemistry and autoradiography.…”

mentioning

confidence: 99%

“…Ferrokinctic and porphyrin excretion studies have shown that ineffective erythropoiesis is an important factor in the pathogenesis of the anaemia in P-thalassaemia (Sturgeon & Finch, 1957; Grinstein ct al, 1960;Malamos et al, 1961) and indicate that, in addition to the impairment of P-chain synthesis, there must also be a disorder of erythroblast proliferation in this disease. This paper describes the results of a study of erythroblast proliferation in P-thalassaemia, using a combination of quantitative cytochemistry and autoradiography.…”

mentioning

confidence: 99%

Proliferation of Erythroblasts in Beta‐Thalassaemia

et al. 1970

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Erythropoiesis has been studied by the technique of combined Feulgen microspectrophotometry and 3H-TdR autoradiography in nine children with Pthalassaemia. A major kinetic aberration was demonstrated in the early polychromatic erythroblasts, which showed an accumulation in G, and a marked decrease in the proportion of cells in DNA synthesis, indicating an arrest of proliferation in these cells. In scven of the cases studied there was also an increased frequency of G, cells relative to the small number in DNA synthesis, suggesting a failure to enter mitosis.The death of these arrested cells could account for the ineffectiveness of erythropoiesis in this disease. Low serum folate levels were found in all the patients who were not already on folate therapy, but the disturbance in cell proliferation persisted after the administration of folic acid.Ferrokinctic and porphyrin excretion studies have shown that ineffective erythropoiesis is an important factor in the pathogenesis of the anaemia in P-thalassaemia (Sturgeon & Finch, 1957; Grinstein ct al, 1960;Malamos et al, 1961) and indicate that, in addition to the impairment of P-chain synthesis, there must also be a disorder of erythroblast proliferation in this disease. This paper describes the results of a study of erythroblast proliferation in P-thalassaemia, using a combination of quantitative cytochemistry and autoradiography. The value of this technique in the detection of disturbances in cell proliferation has been established in previous studies of pernicious anaemia and sideroblastic anaemia (Menzies ct al, 1966;Bock et al, 1967;Yoshida et a/, 1968;Wickramasinghe et al, 1968a Wickramasinghe et al, , b, 1969. MATERIAL A N D METHODSPatients. Eight patients with 8-thalassaemia major and one patient with homozygous thalassaemia intermedia (Case 9) have been studied; these children were either Greek or Turkish Cypriots resident in London. The pertinent haematological data at the time of the initial bone-marrow analysis are summarized in Table I. Apart from Case 9, who was receiving regular folate therapy, all patients had serum folate levels below the normal range. In six of these patients, the investigation was repeated following the oral administration of folic acid, 13 mg daily for at least 6 weeks.Cell cycle distribution. The method used for the determination of the distribution of erythroblasts in interphase-G,, S, G,, and U-was a modification of that described previously (Wickramasinghe et al, 1968a). GI represents post-mitotic cells with a diploid (2. ) DNA 719

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“…Comparison of the Bn: Pn: LPn ratios with reported ratios in other species [1,5] indicates that the Tc and Tt of cows is shorter for the younger cells and longer for the more mature cells. These variations among species are not sur prising since the life span of the erythrocyte and the corresponding rates of erythropoiesis are different among the species.…”

Section: Discussionmentioning

confidence: 92%

Kinetics of Erythroid Bone Marrow Cells of Normal and Porphyric Calves in vitro

Rudolph¹,

Kaneko²

1971

Acta Haematol

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The in vitro proliferation and maturation of bone marrow cells in erythropoietic porphyria of cows was studied using ³H-thymidine labeling and autoradiography. Ratios of basophilic normoblast: polychromatic normoblast:late polychromatic normoblast: and orthochromatic normoblast were 1:3:8:19 for normal marrow and 1:3:12:32 for porphyric marrow. The turnover times for the basophilic normoblast: polychromatic normoblast: late polychromatic normoblast were 10:17:31 h in the normals as compared to 12:16:26 h in the porphyries. The cell cycle times and DNA synthetic times were correspondingly similar

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In-vitro Study of DNA-Synthesis Time and Cell-Cycle Time in Erythrocyte Precursors of Normal and Thalassaemic Subjects, Using a <sup>3</sup>H- and <sup>14</sup>C-Thymidine Double Labelling Technique

Cited by 31 publications

References 7 publications

Erythropoietic Cell Proliferation in Different Clinical States of <i>β</i>-Thalassaemia

Erythropoietic Cell Proliferation in Different Clinical States of <i>β</i>-Thalassaemia

Proliferation of Erythroblasts in Beta‐Thalassaemia

Kinetics of Erythroid Bone Marrow Cells of Normal and Porphyric Calves <i>in vitro</i>

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