In vitro study of doxorubicin-induced oxidative stress in spermatogonia and immature Sertoli cells

Tremblay, Amélie; Delbès, Géraldine

doi:10.1016/j.taap.2018.04.014

Cited by 25 publications

(24 citation statements)

References 79 publications

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“…FSH: follicle stimulating hormone, LH: luteinizing hormone, SSC: spermatogonial stem cell. [24][25][26][27][28][29][30]77,81,83], spermatogonia and spermatocytes [24,43,[46][47][48]52,53,55,89,[96][97][98], spermatozoa [56,62,98,[108][109][110][111]125,126,[129][130][131]135,136] and progeny [55,[109][110][111][143][144][145][146][147][148][149]151] following chemotherapy administration during childhood and adulthood. Animal species and the age at which the chemotherapy treatments achieved are specified in parentheses.…”

Section: Discussionmentioning

confidence: 99%

“…The Sertoli cell survival can be explained by a mechanism of apoptosis and autophagy inhibition [25]. However, an increased oxidative stress has been measured in an immature Sertoli cell line (Ser-W3) after doxorubicin exposure [26]. Moreover, the administration of DNA topoisomerase II inhibitor led to morphological and nuclear alterations, resulting in cytoplasmic vacuolization and abnormal chromatin condensation, respectively [27,28].…”

Section: Effects Of Chemotherapy On Somatic Cellsmentioning

confidence: 99%

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Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility

Delessard

Saulnier

Rives

et al. 2020

IJMS

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Over the last decade, the number of cancer survivors has increased thanks to progress in diagnosis and treatment. Cancer treatments are often accompanied by adverse side effects depending on the age of the patient, the type of cancer, the treatment regimen, and the doses. The testicular tissue is very sensitive to chemotherapy and radiotherapy. This review will summarize the epidemiological and experimental data concerning the consequences of exposure to chemotherapy during the prepubertal period or adulthood on spermatogenic progression, sperm production, sperm nuclear quality, and the health of the offspring. Studies concerning the gonadotoxicity of anticancer drugs in adult survivors of childhood cancer are still limited compared with those concerning the effects of chemotherapy exposure during adulthood. In humans, it is difficult to evaluate exactly the toxicity of chemotherapeutic agents because cancer treatments often combine chemotherapy and radiotherapy. Thus, it is important to undertake experimental studies in animal models in order to define the mechanism involved in the drug gonadotoxicity and to assess the effects of their administration alone or in combination on immature and mature testis. These data will help to better inform cancer patients after recovery about the risks of chemotherapy for their future fertility and to propose fertility preservation options.

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Section: Discussionmentioning

confidence: 99%

Section: Effects Of Chemotherapy On Somatic Cellsmentioning

confidence: 99%

Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility

Delessard

Saulnier

Rives

et al. 2020

IJMS

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“…Nevertheless, an interesting finding of our research was that SMI significantly suppressed ATP production in DOX-treated H9c2 cardiomyocytes, although it exhibited protective effect on mitochondrial membrane potential as well as inhibitive effect on oxidative stress. Of note, during the mitochondrial oxidative phosphorylation, with the production of ATP, 1-2% of the O 2 consumed by mitochondria receives electron leaked by the components of the mitochondrial ETC and gets converted into ROS (Chen Q. et al, 2003;Tremblay and Delbes, 2018). In addition, a previous study has found that Ginseng or ginsenosides may present neuroprotective activity by reducing formation of ROS and ATP through suppressing the ATP synthase activity (Kong et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Shenmai Injection Protects Against Doxorubicin-Induced Cardiotoxicity via Maintaining Mitochondrial Homeostasis

Wang

et al. 2020

Front. Pharmacol.

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MATERIALS AND METHODS Reagents and Chemicals SMI was purchased from CTQ Pharmaceutical Group Co. Ltd. (Hangzhou, China), the same batch as previous study (Yu et al., 2019). Cell culture supplies were purchased from Gibco (Grand Island, NY, USA). Anti-PI3K, anti-Akt, anti-Phospho-Akt (Ser473), anti-GSK-3b, anti-Phospho-GSK-3b (Ser9), anti-GAPDH, anti-AMPKa, anti-Phospho-AMPKa (Thr172), anti-Phospho-DRP1 (Ser616), anti-Phospho-DRP1 (Ser637), anti-Bax, anti-Bcl-2, anti-Caspase3, and anti-cleaved-Caspase3 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-OPA1, anti-MFN2, and anti-FIS1 were purchased from Abcam (Cambridge, MA, UK). Anti-DRP1 and anti-MFN1 were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA). MitoSOX Red, MitoTracker Green, and MitoTracker Deep Red were purchased from Invitrogen (Eugene, USA).

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“…Moreover, the etiology remains unknown and the infertility is classified as idiopathic in more than 50% of male infertility cases [4,[12][13][14]. A new but important cause has emerged next to the conventional origins of male infertility: oxidative stress (OS)-described as the main etiological factor causing sperm DNA damage [15][16][17].Among the biochemical parameters of disease diagnosis soundness, enzymes arise as suitable biomolecules not only for diagnosis but also for monitoring purposes. Considering the links established between oxidative processes and infertility in human beings, enzymes related to these processes seem appropriate candidates for studies on human fertility.…”

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confidence: 99%

mentioning

confidence: 99%

Catalase as a Molecular Target for Male Infertility Diagnosis and Monitoring: An Overview

et al. 2020

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Catalase (CAT) stands out as one of the most efficient natural enzymes when catalysing the split of H2O2 into H2O and O2; H2O2 is one of the reactive oxygen species (ROS) involved in oxidative stress, a process closely related to aging and several health disorders or diseases like male infertility. Some studies have correlated H2O2 with male infertility and catalase with fertility restoration. However, the number of studies conducted with human beings remains scarce. Considering the use of CAT as a molecular target for biochemical analysis, this review summarises the current knowledge on how CAT influences human beings’ male fertility. Thus, three different databases were consulted—Scopus, PubMed and WOS—using single keywords and combinations thereof. A total of 40,823 articles were identified. Adopting inclusion and exclusion criteria, a final database of 197 articles served to conduct this work. It follows from this analysis that CAT could play an important role in male fertility and could become a good target for male infertility diagnosis and monitoring. However, that potential role of CAT as a tool in diagnosis must be confirmed by clinical trials. Finally, guidelines are suggested to reinforce the use of CAT in daily clinical tests for male fertility diagnosis and monitoring.

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In vitro study of doxorubicin-induced oxidative stress in spermatogonia and immature Sertoli cells

Cited by 25 publications

References 79 publications

Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility

Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility

Shenmai Injection Protects Against Doxorubicin-Induced Cardiotoxicity via Maintaining Mitochondrial Homeostasis

Catalase as a Molecular Target for Male Infertility Diagnosis and Monitoring: An Overview

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