In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

Abstract

Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

Are Metal Ions That Make up Orthodontic Alloys Cytotoxic, and Do They Induce Oxidative Stress in a Yeast Cell Model?

“…Although several studies are available in the literature regarding the biocompatibility and potential cytotoxic impact of the metal ions released from implants [21][22][23], restorations [24][25][26] and orthodontic appliances such as Fe, Ni, Cr, Co, Ti, and Mo [27][28][29], no previous report has simulated orthodontic alloys with metal ion mixtures to evaluate their potential. Cultured animal or human cell lines such as fibroblasts [28] and osteoblasts [30] have been mainly used to evaluate the cytotoxic effect of orthodontic appliances, and currently, only a few studies [31,32] have included yeast for these type of studies. Limberger et al [31] compared the results on S. cerevisiae with other cell lines and concluded that microorganisms are a reliable model for cytotoxic testing of orthodontic materials.…”

In vitro study of the orthodontic mini-implants influence on the growth of human osteoblasts

“…Bueno & Basting evaluated the influence of Ti alloy mini-implants on osteoblasts and concluded that their proliferation has increased from 24 to 72 hours. Also, cell adhesion at 72 hours suggested the presence of bone remodeling, required for osseointegration initiation [ 22 ]. Malkoҫ et al studied the cytotoxicity in five types of mini-implants made from two different alloys (stainless steel and Ti alloy) using a real-time cell analysis system.…”

In vivo determination of aluminum, cobalt, chromium, copper, nickel, titanium and vanadium in oral mucosa cells from orthodontic patients with mini-implants by Inductively coupled plasma-mass spectrometry (ICP-MS)

“…Mini-screws became widely used to bear orthodontic and orthopaedic loads, becoming effective orthodontic anchorage devices [34][35]. Firstly, the material of choice to manufacture mini-implants was commercially pure Titanium (cp Ti), but in order to improve its mechanical properties, some elements such as aluminum (Al), vanadium (V) and iron (Fe) have been incorporated [36]. Many new systems were introduced, such as onplants [37], palatal implants [38], bicortical screws [39], miniplates [40], miniplates with a tube [41], and the zygoma anchorage system [42].…”