In Vitro Study of Potato (Solanum tuberosum L.) Tolerant to the Drought Stress

Abstract

In vitro preliminary studies is candidate tolerant of potato (Solanum tuberosum L.) to the drought stress. This study aimed to determine the characters of potato after being exposed to in vitro drought stress conditions using Polyethylene Glycol PEG. This research was conducted at the Tissue Culture Laboratory, UPT Balai Benih Induk Hortikultura Dinas Pertanian Provinsi Sumatera Utara, in Medan and other places in January 2015 until May 2015. This study used Completely Randomized non Factorial Design namely PEG (P) comprising of two levels, namely: P1 = 5 000 mg ⋅ L −1 , P2 = 6 000 mg ⋅ L −1 . The results showed that increasing the concentration of PEG resulted in reduction of the percentage of plantlets survival, reduced plantlets height and plantlets dry weight but the increase in the total protein and leaf chlorophyll. Although the banding pattern is relatively the same, there is a brighter visible banding pattern on potato plantlets with OPAA 09 in the range of 65 bp to 75 bp with sequences of GTGGGTGCCA.

Responses of Potato (Solanum tuberosum L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture

“…In field and greenhouse and also pot experiments, drought affected all physiological and agronomic traits of the potato cultivars studied [35,36]. Osmotic stress applied to model drought stress under in vitro conditions also resulted in significant changes in all studied traits in potato, e.g., survival rate, shoot length, fresh and dry weight, and root number and length [37,38]. However, no difference could be revealed between genotypes if the evaluation was based only on the measured parameters, even though significant inhibition of the growth of in vitro shoots and roots as an effect of osmotic stress in Solanum species was observed [32,39].…”

“…Changes in morphological and physiological traits affected by osmotic stress are similar under in vivo conditions to those observed in in vitro tissue cultures. Survival rate, shoot length, fresh and dry weight, and number and length of roots are the most commonly affected characteristics [35][36][37][38]. Despite the fact that the growth parameters of the plantlets were strongly influenced by osmotic stress during the stress resistance experiments-in fact, they were most often inhibited-it was possible to differentiate the genotypes according to their stress tolerance only if a stress index was formed based on the measured growth parameters [32,39,40].…”