In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes

Yu, Guijuan; Ou, Weihao; Ai, Qinghui; Zhang, Wenbing; Mai, Kangsen

doi:10.1016/j.fsirep.2021.100031

Cited by 2 publications

(1 citation statement)

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“…According to our previous study ( 34 ), the isolation and culture of primary intestinal epithelial cells were performed. Briefly, the starved turbots were anesthetized with eugenol (1:10000, Shanghai Reagent Corp, China), wiped, and disinfected with 75% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy

et al. 2022

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Vitamin D3 (VD3) participated widely in the nuclear factor-κB (NF-κB)-mediated inflammation, apoptosis, and autophagy through the vitamin D receptor (VDR). However, the molecular mechanisms remain not understood in teleost. The present study investigated the functions of VD3/VDR on intestinal inflammation, autophagy, and apoptosis of turbot in vivo and in vitro. Triple replicates of 30 fish were fed with each of three diets with graded levels of 32.0 (D0), 1012.6 (D1), and 3978.2 (D2) IU/kg VD3. Obvious intestinal enteritis was observed in the D0 group and followed with dysfunction of intestinal mucosal barriers. The intestinal inflammatory response induced by VD3 deficiency was regulated by the NF-κB/inflammasome signalling. The promotion of intestinal apoptosis and suppression of intestinal autophagy were also observed in the D0 group. Similarly, VD3 deficiency in vitro induced more intense inflammation regulated by NF-κB/inflammasome signalling. The mutually exclusive apoptosis and autophagy were also observed in the group without 1,25(OH)2D3in vitro, accompanied by similar changes in apoptosis and autophagy increased apoptosis. The gene expression of VDRs was significantly increased with the increasing VD3 supplementation both in vivo and in vitro. Moreover, VDR knockdown in turbot resulted in intestinal inflammation, and this process relied on the activation of inflammasome mediated by NF-κB signalling. Simultaneously, intestinal apoptosis was promoted, whereas intestinal autophagy was inhibited. In conclusion, VD3 deficiency could induce intestinal inflammation via activation of the NF-κB/inflammasome pathway, intestinal apoptosis, and autophagy formed a mutually exclusive relation in teleost. And VDR is the critical molecule in those processes.

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Section: Methodsmentioning

confidence: 99%

Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy

et al. 2022

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Dietary sodium butyrate administration alleviates high soybean meal-induced growth retardation and enteritis of orange-spotted groupers (Epinephelus coioides)

Qin²,

Song³

et al. 2022

Front. Mar. Sci.

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An 8-week feeding trial was conducted to investigate whether dietary sodium butyrate (SB) administration alleviates growth reduction and enteritis of orange-spotted grouper (Epinephelus coioides) caused by high soybean meal (SBM) feeding. The control diet (FM diet) was formulated to contain 48% protein and 11% fat. Soybean meal was used to replace 60% FM protein in FM diet to prepare a high SBM diet (HSBM diet). Sodium butyrate (SB) at 0.1%, 0.2%, and 0.3% were added to HSBM diets to prepare three diets. Triplicate groups of 30 groupers (initial weight: 33.0 ± 0.3 g) were fed one of the diets twice daily, to apparent satiety. HSBM diets had lowered growth rate and feed efficiency vs FM diets (P <0.05). Growth rate and feed efficiency were improved by dietary SB administration and were in a dose-dependent manner (P <0.05). A similar pattern to the growth rate was observed for plasma LDL-C and gut digestive activity of lipase, trypsin, and protease, but the opposite trend was observed for intestinal contents of D-lactic acid and endotoxin, in response to dietary SB inclusion levels (P >0.05). The muscular thickness in the middle and distal intestines in SB-treated diets were higher than that in HSBM diets (P <0.05). The mRNA levels of intestinal pro-inflammatory cytokines IL-8, IL-1β, IL-12 and TNF-α had a decreasing trend, and the mRNA level of intestinal anti-inflammatory cytokine TGF-β1 had the opposite trend, with increasing SB inclusion levels (P < 0.05). The above results indicate that dietary SB intervention could enhance growth and feed utilization of groupers with SBM-induced enteritis by promoting intestinal digestive enzyme activities, reducing mucosa permeability, maintaining the integrity of intestinal morphology and attenuating the intestinal inflammatory response.

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In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes

Cited by 2 publications

References 41 publications

Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy

Vitamin D3 deficiency induced intestinal inflammatory response of turbot through nuclear factor-κB/inflammasome pathway, accompanied by the mutually exclusive apoptosis and autophagy

Dietary sodium butyrate administration alleviates high soybean meal-induced growth retardation and enteritis of orange-spotted groupers (Epinephelus coioides)

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