Background and objective: Lasers can be successfully used for the modification of biological tissues. In den tistry, an increase in strength and the chemical pro perties of enamel with a laser in order to prevent tooth decay appears to be quite promising. We investigated microhardness and acid resistance of human tooth enamel in vitro before and after treatment by YLF:Er laser radiation with subablative energy density. Scan ning electron microscope (SEM) study of the enamel surface before and after laser treatment was carried out. A possible mechanism of the laser radiation effect on the microhardness and acid resistance of enamel is discussed. Materials and methods: Enamel of the tooth crown was investigated in vitro. A pulsed diodepumped YLF:Er laser (λ = 2.84 μm) was used operating in freerunning mode with an energy density on the enamel surface of about 2 J/cm 2 . A pattern was created on the enamel surface, every point of the pattern being formed by the impact of 100 laser pulses. Microhardness before (HV e ) and after (HV te ) laser treatment was measured by the Vickers hardness test. Microhardness of intact and lasertreated enamel were measured after mechanical cleaning with an abrasive toothpaste at room temperature to estimate the wear resistance (abrasion resistance). Gel Etchant (Kerr, Scafati, Italy) was applied to intact and lasertreated enamel. The exposure time during which intact (t e ) and treated (t te ) enamel was destroyed was determined to esti mate acid resistance. Scanning microscopy of enamel before and after laser treatment was carried out using a SEM. The original SEM image processing technique allowed us to estimate the porosity of the enamel surface before and after laser treatment. Results: The measured microhardness of intact enamel of human tooth crown was HV e = 310 ± 10, and enamel microhardness after laser treatment reached HV te = 375 ± 10. However, even after 45 min mechanical cleaning with abrasive toothpaste at room temperature, lasertreated enamel microhardness was higher than intact enamel microhardness. Measured exposure time t e was 3 ± 1 s, and the exposure time t te was 180 ± 15 s. The porosity of the intact enamel surface is almost three times greater than the porosity of lasertreated enamel. Conclusion: In this paper it was found that subablative YLF:Er laser radiation modifies the intact enamel in such a way that the enamel microhardness increases and can exceed the intact enamel microhardness by 20%. Laser treated enamel can resist abrasive impact during a time equivalent to 3 years of standard dental hygiene. Laser treated enamel can resist an external aggressive medium containing 37.5% phosphoric acid significantly longer than intact enamel. The observed improvement in the mechanical and chemical properties of the enamel can be explained by a significant reduction in enamel porosity after laser radiation impact.
ZusammenfassungHintergrund und Zielsetzung: Laser können erfolgreich für die Modifikation von biologischen Geweben einge setzt werden. In der Zahnmedizin ...