In vitro study of transmission of bacteria from contaminated metal models to stone models via impressions

Sofou, Afrodite; Larsen, Tove; Owall, B; Fiehn, Nils‐Erik

doi:10.1007/s00784-002-0174-3

Cited by 33 publications

(1 citation statement)

References 11 publications

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“…Until 1991, rinsing impressions under running water was the recommended practice and has been shown to reduce the count of microorganisms present on the impression surface by 40% to 90%, but a measurable bacterial load still remains on impressions and can be transferred to casts. 17 Hence, disinfection becomes mandatory. Rinsing is also considered beneficial as it removes organic matter that may prevent exposure of the impression surface to the disinfectant and compromises the activity Journal of International Medicine and Dentistry 2016; 3(3): 151-160 of disinfectant, which can have their action decreased or even inactivated in the presence of organic remnants.…”

Section: Discussionmentioning

confidence: 99%

4. Comparison of various methods of disinfecting irreversible hydrocolloid impressions using chlorhexidine gluconate: Assessment of antimicrobial efficacy & dimensional changes

Gupta¹,

Agarwal²,

Tiwari³

et al. 2016

JIMD

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The aim of the study was to investigate different procedures to check for the most effective disinfection method and most dimensionally accurate impressions post-treatment with disinfectant. Five alginate impressions, each of thirty-two dentate patients were made with 24 hours interval. Impressions were divided into five categories: Group I included alginate impression with only water rinsing (Control). Group II were with immersion in chlorhexidine gluconate solution, Group III were spraying and Group IV were impression of alginate mixed with chlorhexidine solution. Group V comprised of impressions taken after pre-procedural mouth rinsing. Samples were inoculated in blood agar media for 3 days at 37°C. The impressions were poured in type III dental stone. The inter-molar distance, inter-canine distance, the antero-posterior distance and the depth of the cast were measured with Digital Vernier calipers. The results indicate the presence of highest number of bacterial colonies on Group I impression i.e. the control group. Least bacterial count was found in Group V followed by Group III, Group II and Group IV. Highest dimensional changes were seen in Group IV followed by Group III and Group II casts. Group V was found to be most dimensionally stable. Within the limitations of the study, it was concluded that the most effective and accurate method of disinfection is pre-procedural rinsing followed by spraying and immersion. Internal disinfection was found to be the least effective method with high bacterial count as well as high dimensional changes.

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Section: Discussionmentioning

confidence: 99%

4. Comparison of various methods of disinfecting irreversible hydrocolloid impressions using chlorhexidine gluconate: Assessment of antimicrobial efficacy & dimensional changes

Gupta¹,

Agarwal²,

Tiwari³

et al. 2016

JIMD

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Microbial contamination of laboratory constructed removable orthodontic appliances

et al. 2014

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractObjectives: To determine whether laboratory constructed removable orthodontic appliances are free from microbial contamination prior to clinical use and to evaluate the dental hospital cross-infection procedures to ensure patient-derived contamination does not enter the construction process, thereby propagating a cycle of cross-contamination. Materials and Methods:The construction process of removable orthodontic appliances from three individuals was evaluated at every stage, from impression to final delivery of the appliance using molecular microbiological techniques. The bacterial profiles at each stage of appliance construction were obtained using Denaturing Gradient Gel Electrophoresis, along with the bacterial profiles of the three participants' saliva. This enabled the bacterial profiles found at each stage of construction to be compared directly with the saliva of the person for whom the appliance was being constructed. Bacteria were identified at each stage by using 16S rDNA PCR amplification and sequence phylogeny. Results:There was no evidence of bacterial cross-contamination from patients to the laboratory. The current process of disinfection of impression appears to be adequate. Contamination was found on the final removable appliances (0.97x10 2 -1.52x10 3 cfu ml -1) and this contamination occurred from within the laboratory itself. Conclusions:Every effort is made to reduce potential cross-infection to patients and dental professionals. Newly constructed removable appliances to be shown not to be free from contaminated with bacteria prior to clinical use, but this contamination is environmental. Further studies would be required to determine the level of risk this poses to patients.Clinical significance: Dental professionals have a duty of care to minimise or eradicate potential risks of crossinfection to patients and other members of the team. To date much less attention has been paid to contamination from the orthodontic laboratory so contamination and infection risks are unknown.

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Biological properties of experimental dental alginate modified for self-disinfection using green nanotechnology

Singer,

Karacic,

Szekat

et al. 2023

Clin Oral Invest

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Objectives Disinfection of alginate impression materials is a mandatory step to prevent cross-infection in dental clinics. However, alginate disinfection methods are time-consuming and exert a negative impact on accuracy and mechanical properties. Thus, this study aimed to prepare disinfecting agents (CHX and AgNO3) and silver nanoparticles reduced by a natural plant extract to produce a self-disinfecting dental alginate. Methods Conventional alginate impression material was used in this study. Silver nitrate (0.2% AgNO3 group) and chlorohexidine (0.2% CHX group) solutions were prepared using distilled water, and these solutions were later employed for alginate preparation. Moreover, a 90% aqueous plant extract was prepared from Boswellia sacra (BS) oleoresin and used to reduce silver nitrate to form silver nanoparticles that were incorporated in the dental alginate preparation (BS+AgNPs group). The plant extract was characterized by gas chromatography/mass spectrometry (GC/MS) analysis while green-synthesized silver nanoparticles (AgNPs) were characterized by UV-visible (UV–vis) spectroscopy and scanning electron microscopy (SEM). An agar disc diffusion assay was used to test the antimicrobial activity against Candida albicans, Streptococcus mutans, Escherichia coli, methicillin-resistant and susceptible Staphylococcus aureus strains, and Micrococcus luteus. Agar plates were incubated at 37 ± 1 °C for 24 h to allow microbial growth. Diameters of the circular inhibition zones formed around each specimen were measured digitally by using ImageJ software. Results Chemical analysis of the plant extract revealed the presence of 41 volatile and semi-volatile active compounds. UV–Vis spectrophotometry, SEM, and EDX confirmed the formation of spherical silver nanoparticles using the BS extract. CHX, AgNO3, and the BS+AgNPs modified groups showed significantly larger inhibition zones than the control group against all tested strains. BS+AgNPs and CHX groups showed comparable efficacy against all tested strains except for Staphylococcus aureus, where the CHX-modified alginate had a significantly higher effect. Conclusions and clinical relevance CHX, silver nitrate, and biosynthesized silver nanoparticles could be promising inexpensive potential candidates for the preparation of a self-disinfecting alginate impression material without affecting its performance. Green synthesis of metal nanoparticles using Boswellia sacra extract could be a very safe, efficient, and nontoxic way with the additional advantage of a synergistic action between metal ions and the phytotherapeutic agents of the plant extract.

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In vitro study of transmission of bacteria from contaminated metal models to stone models via impressions

Cited by 33 publications

References 11 publications

4. Comparison of various methods of disinfecting irreversible hydrocolloid impressions using chlorhexidine gluconate: Assessment of antimicrobial efficacy & dimensional changes

4. Comparison of various methods of disinfecting irreversible hydrocolloid impressions using chlorhexidine gluconate: Assessment of antimicrobial efficacy & dimensional changes

Microbial contamination of laboratory constructed removable orthodontic appliances

Biological properties of experimental dental alginate modified for self-disinfection using green nanotechnology

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