In vitro survival and lipid peroxidation status of rabbit spermatozoa after both chilled and frozen storage in lycopene enriched extenders

Rosato, M. P.; Iorio, Michele Di; Manchisi, A.; Gambacorta, M.; Petrosino, G.; Centoducati, Gerardo; Santacroce, Maria Pia; Iaffaldano, Nicolaia

doi:10.1016/j.livsci.2012.03.006

Cited by 14 publications

(14 citation statements)

References 19 publications

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“…Our data are in agreement with previous in vitro reports on chilled or frozen rabbit, turkey and fowl semen (Rosato et al 2012a;Rosato et al 2012b;Mangiagalli et al 2007) suggesting that LYC may have beneficial effects on sperm motion characteristics as a result of its ROS-quenching abilities and protective effects against oxidative damage to 100.00 ± 0.00 147.50 ± 6.26*** AI = acrosomal integrity, ALH = amplitude of lateral head displacement, BCF = beat cross frequency, MA = Mitochondrial activity, MI = membrane integrity, PM = progressive motility, STR = path straightness, VAP = average path velocity, VCL = velocity of curvilinear motion, VSL = linear velocity *P < 0.05; **P < 0.01; ***P < 0.001 .45 ± 0.81 2.43 ± 0.56*** *P < 0.05; **P < 0.01; ***P < 0.001 structures crucial for the functional activity of male reproductive cells. Furthermore, the protective effects of LYC on mitochondrial metabolism recorded in our study complement the findings of Bucak et al (2015) who observed a marked improvement in the preservation of the sperm mitochondrial membrane potential following LYC supplementation.…”

Section: Discussionsupporting

confidence: 93%

“…Intracellular antioxidant capacity fails to provide protection against the oxidative stress associated with the toxic effects of ROS following freezing and thawing (Ball 2008;Rosato et al 2012a;Rosato et al 2012b). The cold shock inflicted on cryopreserved sperm is linked to oxidative damage to crucial structural and functional biomolecules, followed by alterations to intracellular signalling pathways and engagement of apoptosis (Ball 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Previous in vivo as well as in vitro reports have demonstrated that LYC may improve sperm motility, viability, morphology and testicular oxidative balance following exposure to medication (Atessahin et al 2006a;Atessahin et al 2006b), organic pollutants (Raj et al 2012;Tamiselvan et al 2013), oxidative stress promoters (Zini et al 2010;Tvrda et al 2016a) or mycotoxins (Tas et al 2010). At the same time, pivotal studies have emphasised the potential of LYC as a suitable antioxidant supplement to extenders for mammalian or avian semen (Mangiagalli et al 2007;Uysal and Bucak 2007;Rosato et al 2012a;Rosato et al 2012b;Bucak et al 2015).…”

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confidence: 99%

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Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Tvrdá¹,

Mackovich²,

Greifová³

et al. 2017

Vet. Med. - Czech

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Reactive oxygen species overgeneration as a side effect of semen cryopreservation may lead to lipid peroxidation, protein degradation, DNA fragmentation and cell death, resulting in a decrease of sperm survival and fertilisation ability. Lycopene has been proposed as a potential supplement to semen extenders because of its antioxidant properties. The aim of this study was to evaluate the effects of lycopene on the structural integrity, functional activity and selected oxidative stress parameters of cryopreserved bovine sperm. Thirty bovine ejaculates were split into two aliquots and diluted with a commercial semen extender supplemented with 1.5 mmol/l lycopene or containing no supplement (control), cooled down to 4 °C, frozen and kept in liquid nitrogen. Prior to experiments, frozen straws were thawed at 37 °C for 20 s. Lycopene addition resulted in a higher sperm motility (P < 0.001), progressive motility (P < 0.001) and all secondary motion characteristics (P < 0.001 with respect to the average path velocity, linear velocity, velocity of curvilinear motion, beat cross frequency, path straightness and linearity; P < 0.01 in the case of the amplitude of lateral head displacement). Furthermore, lycopene exhibited protective effects on the sperm membrane (P < 0.05) and acrosomal (P < 0.01) integrity in comparison to control. An assay for metabolic function revealed that lycopene supplementation to the cryopreservation medium resulted in a higher preservation of the sperm mitochondrial activity (P < 0.001). Reactive oxygen species production as well as intracellular superoxide generation were decreased following lycopene addition (P < 0.01 in the case of reactive oxygen species; P < 0.001 with respect to superoxide production). Finally, the presence of lycopene led to a decrease in protein carbonyl production (P < 0.01), lipid peroxidation (P < 0.001) as well as oxidative DNA damage (P < 0.05) when compared to control. In conclusion, lycopene exhibited significant reactive oxygen species-trapping and antioxidant properties which may prevent oxidative damage to frozen-thawed sperm, and, thus, enhance the post-thaw vitality of male reproductive cells in cattle breeding.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Tvrdá¹,

Mackovich²,

Greifová³

et al. 2017

Vet. Med. - Czech

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“…Frozen‐thawed semen is more easily peroxidized than fresh sperm. Intracellular antioxidant capacity fails to provide protection against oxidative damage and the potential toxic effects of free radicals following freeze–thawing (Trinchero et al ., ; Rosato et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Lycopene and resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity

et al. 2014

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We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots and diluted at 37 °C with base extenders containing lycopene (1 × 10(-3) g ml(-1) ) and resveratrol (1 mm), and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The supplementation of the semen extender with lycopene and resveratrol increased the percentages of post-thawed computer-assisted sperm analysis (CASA) motility (55.8 ± 3.8 and 61.9 ± 4.0%) and progressive motility (38 ± 2.4 and 37 ± 8.8), compared with the controls (50.7 ± 2.65 and 33.3 ± 3.74%, respectively, P < 0.05). Resveratrol provided a higher ALH (4.3 ± 0.1), in comparison with the control (3.9 ± 0.3, P < 0.05). The supplementation of the semen extender with lycopene and resveratrol produced a higher mitochondrial activity (24.6 ± 2.9 and 30.1 ± 6.5% respectively), compared with that of the control (11.8 ± 9.5%, P < 0.05). It was determined that both antioxidants resulted in a lower percentage of sperm with damaged DNA than that of the control (P < 0.05). Sperm motion characteristics except for ALH, acrosome integrity, sperm viability and oxidative stress parameters were not affected by the adding of lycopene and resveratrol.

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“…Several studies had been conducted to improve the preservation protocols and the extender composition [1][2][3][4][5][6][7][8][9][10]. Unfortunately, rabbit spermatozoa had a restricted capacity to withstand the chilling [5,6] or frozen storage [11] is limited.…”

Section: Introductionmentioning

confidence: 99%

Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE)

El-Seadawy

El-Nattat

El-Tohamy

et al. 2017

APJR

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In vitro survival and lipid peroxidation status of rabbit spermatozoa after both chilled and frozen storage in lycopene enriched extenders

Cited by 14 publications

References 19 publications

Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation

Lycopene and resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity

Preservability of rabbit semen after chilled storage in tris based extender enriched with different concentrations of Propolis ethanolic extract (PEE)

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