In vitro susceptibilities to amphotericin B, itraconazole, and miconazole of filamentous fungi isolated from patients with cystic fibrosis

Hennequin, Christophe; Benailly, N; Silly, C; Sorin, M; Scheinmann, P.; Lenoir, G.; Gaillard, Jean‐Louis; Berche, Patrick

doi:10.1128/aac.41.9.2064

Cited by 26 publications

(14 citation statements)

References 13 publications

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“…Only 10 of the 23 patients with fungal MOE initially presented with otitis externa without signs of middle ear or mastoid involvement (cases 1,4,7,13,15,16,19,20,22, and 23).…”

Section: Review Of Literaturementioning

confidence: 99%

Fungal Malignant Otitis Externa Due to Scedosporium Apiospermum

Yao

Messner²

2001

Ann Otol Rhinol Laryngol

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Malignant otitis externa (MOE) is an infection of the external auditory canal that invades the skull base. Aspergillus species fungi were the pathological organism in 21 of 23 reported cases of fungal MOE. We report on a 21-year-old man with end-stage acquired immunodeficiency syndrome (AIDS) and fungal MOE caused by Scedosporium apiospermum. Fungal MOE is most common in patients with end-stage AIDS and hematologic malignancies. Granulation tissue is not a common finding in these patients, and the infectious process often starts in the mastoid air cells or middle ear space, as opposed to the external auditory canal. Surgical debridement and amphotericin B are the mainstays of therapy; resolution of the infection depends greatly on the severity of the underlying disease.

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“…Only 10 of the 23 patients with fungal MOE initially presented with otitis externa without signs of middle ear or mastoid involvement (cases 1,4,7,13,15,16,19,20,22, and 23).…”

Section: Review Of Literaturementioning

confidence: 99%

Fungal Malignant Otitis Externa Due to Scedosporium Apiospermum

Yao

Messner²

2001

Ann Otol Rhinol Laryngol

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“…Equally, numerous cases of disseminated scedosporiosis with cerebral involvement have been reported after neardrowning, resulting from haematogenous spread from a primary pulmonary site of infection (Katragkou et al 2007). Additionally, S. apiospermum is a therapyrefractory pathogen since it is resistant or poorly susceptible to almost all current antifungals (Hennequin et al 1997). For all these reasons, S. apiospermum is a challenging pathogen and there is an urgent need for a better understanding of the pathogenesis of scedosporiosis.…”

Section: Introductionmentioning

confidence: 99%

Hydroxamate siderophores of Scedosporium apiospermum

et al. 2009

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Scedosporium apiospermum is an emerging pathogen colonizing the airways of patients with cystic fibrosis and causing severe infections in immunocompromised hosts. In order to improve our knowledge on the pathogenic mechanisms of this fungus, we investigated the production of siderophores. Cultivation on CAS medium and specific assays for different classes of siderophores suggested the secretion of hydroxamates. A maximal production was obtained by cultivation of the fungus at alkaline pH in an iron-restricted liquid culture medium. Siderophores were then extracted from the culture filtrate by liquid/liquid extraction, and separated by reverse phase high performance liquid chromatography. Two siderophores, dimerumic acid and Nα-methyl coprogen B, were identified by electrospray ionization-mass spectrometry and MS-MS fragmentation. Finally, comparison of various strains suggested a higher production of Na-methyl coprogen B by clinical isolates of respiratory origin. Studies are initiated in order to determine the potential usefulness of these siderophores as diagnostic markers of scedosporiosis.

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“…Especially, severe and often fatal infections are described after lung transplantation, which constitutes the ultimate treatment of the patients (Symoens et al, 2006;Borghi et al, 2010;Morio et al, 2010;Hirschi et al, 2012;Miraldi et al, 2012). This fungus exhibits a low susceptibility to current systemic antifungal drugs (Hennequin et al, 1997;Alastruey-Izquierdo et al, 2007;Lackner et al, 2012). In this context, defining prevention measures aiming to reduce the exposure of the patients to the fungus is particularly challenging.…”

Section: Introductionmentioning

confidence: 99%

Human‐impacted areas of France are environmental reservoirs of the Pseudallescheria boydii/Scedosporium apiospermum species complex

Rougeron

Schuliar

Leto

et al. 2014

Environmental Microbiology

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Species of the Pseudallescheria boydii/Scedosporium apiospermum complex (PSC) are emerging fungal pathogens able to chronically colonize the airways of patients with cystic fibrosis (CF). As P. boydii was found more frequently colonizing the lungs of CF patients in France than in other European countries in a previous report, the present study was conducted in order to clarify distribution of PSC species in France and to characterize their natural habitat. The highest densities of PSC isolates were found in human-impacted areas, i.e. agricultural areas, fluids obtained from wastewater treatment plants, playgrounds and industrial areas. PSC was not detected from soil samples collected in forests. Most PSC culture-positive soil samples exhibited a pH range of 6-8. Scedosporium dehoogii, the most abundant species, was detected in all human-impacted area types except vineyards, whereas Scedosporium aurantiacum was mostly found in agricultural areas. Pseudallescheria boydii and S. apiospermum were predominantly isolated from seashores and playgrounds respectively. Pseudallescheria minutispora was found only once from a playground. This study highlights potential sources of contamination of the patients, especially in the CF context.

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In vitro susceptibilities to amphotericin B, itraconazole, and miconazole of filamentous fungi isolated from patients with cystic fibrosis

Cited by 26 publications

References 13 publications

Fungal Malignant Otitis Externa Due to Scedosporium Apiospermum

Fungal Malignant Otitis Externa Due to Scedosporium Apiospermum

Hydroxamate siderophores of Scedosporium apiospermum

Human‐impacted areas of France are environmental reservoirs of the Pseudallescheria boydii/Scedosporium apiospermum species complex

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