In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review

Abstract

This review summarises the use of the Ankom DaisyII incubator (ADII; Ankom Technology Corporation Fairport, NY, USA), as presented in studies on digestibility, and its extension to other species apart from ruminants, from its introduction until today. This technique has been modified and adapted to allow for different types of investigations to be conducted. Researchers have studied and tested different procedures, and the main sources of variation have been found to be: the inoculum source, sample size, sample preparation, and bag type. In vitro digestibility methods, applied to the ADII incubator, have been reviewed, the precision and accuracy of the method using the ADII incubator have been dealt with, and comparisons with other methods have been made. Moreover, some hypotheses on the possible evolutions of this technology in non-ruminants, including pets, have been described. To date, there are no standardised protocols for the collection, storage, and transportation of rumen fluid or faeces. There is also still a need to standardise the procedures for washing the bags after digestion. Moreover, some performance metrics of the instrument (such as the reliability of the rotation mechanism of the jars) still require improvement.

High throughput accurate method for estimating in vitro dry matter digestibility of maize silage

“…Some attempts to adapt the Aufrère protocol to achieve a high characterization rate have been made. The use of Daisy technology have been performed [ 23 ]. The Daisy II method can be used on feeds to increase labor efficiency [ 24 ] but from our point of view, it still presents major drawbacks.…”

Comparison of In Vivo and In Vitro Digestibility in Donkeys

“…Digestibility measured in vitro using the Ankom Daisy II Incubator is influenced by different sources of variation, such as type of inoculum, time and temperature of its storage, type of bag, quantity of sample, time of digestion, physical conditions, and diet of the donor [ 41 ]. In our study, most of these conditions were fixed and under control.…”

Temporal Variations in Chemical Composition, In Vitro Digestibility, and Metabolizable Energy of Plant Species Browsed by Goats in Southern Mediterranean Forest Rangeland

“…In vitro dry matter (IVDMD) and organic matter (IVOMD) digestibility were performed using DAISYII Incubator® (ANKOM Technology, Fairport, NY, USA) as described by Mabjeesh et al [ 30 ]. This device is essentially based on the in vivo simulation of digestion [ 31 ]. The rumen liquor for incubation was collected from five goats at a communal slaughterhouse, as described by El Otmani et al [ 32 ].…”