In-vitro testing of bacteriostatic and bactericidal efficacy of commercial disinfectants against Salmonella Infantis reveals substantial differences between products and bacterial strains

Drauch, Victoria; Ibesich, Claudia; Vogl, Claus; Heß, Michael; Heß, Claudia

doi:10.1016/j.ijfoodmicro.2020.108660

Cited by 28 publications

(32 citation statements)

References 21 publications

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“…10% inoculum was inoculated into Monascus purpureus fermentation medium,180r/min, and the mycelium growth was observed after shaking culture at 28℃ for 2 days and shaking culture at 37℃ for 4 days. [10] (1) Cylinder plate method: 200μL Staphylococcus aureus suspension was spread evenly in nutrient agar medium. Five sterile Oxford cups were placed in the middle of the medium and four points uniformly distributed around the medium with aseptic tweezers.…”

Section: Extraction Of Monascinmentioning

confidence: 99%

Study on the extraction and antibacterial activity of Monascin

Gao

Wang

et al. 2021

E3S Web Conf.

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Taking monascin as the research object, monascin was extracted from red kojic rice by ethanol extraction and extracted with 60%, 70% and 80% ethanol respectively. Finally, it was concluded that when the concentration of ethanol was 70%, the extraction rate of monascin was the highest, reached 75.68%. The bacteriostatic experiments of monascin extract and monascin fermentation showed that it had strong inhibitory effect on Staphylococcus aureus and Bacillus subtilis, weak inhibitory ability on Escherichia coli and Aspergillus niger, and no obvious inhibitory effect on the growth of Saccharomyces cerevisiae.

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Section: Extraction Of Monascinmentioning

confidence: 99%

Study on the extraction and antibacterial activity of Monascin

Gao

Wang

et al. 2021

E3S Web Conf.

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“…So far, experimental infection studies with S . Infantis were rare, only based on a single strain and mostly performed in layer-type birds [ 24 , 37 , 38 ]. Therefore, for the first time, an in vivo trial was set up in which infectivity features of two different S .…”

Section: Discussionmentioning

confidence: 99%

“…An increased fitness under various environmental conditions is reported for S. Infantis strains with difficulties of elimination from farms or slaughterhouses, despite extensive cleaning and disinfection (21,22,23). In this respect, we could recently demonstrate that S. Infantis strains which persist on farms were more resistant to disinfectants (24).…”

Section: Introductionmentioning

confidence: 98%

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Infection dynamics of Salmonella Infantis strains displaying different genetic backgrounds – with or without pESI-like plasmid – vary considerably

Drauch

Kornschober

Palmieri

et al. 2021

Emerging Microbes & Infections

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Food-borne infections with Salmonella are among the most common causes of human diseases worldwide, and infections with the serovar Infantis are becoming increasingly important. So far, diverse phenotypes and genotypes of S . Infantis have been reported. Therefore, the present study aimed to investigate the infection dynamics of two different S . Infantis strains in broilers. For this purpose, 15 birds were infected on day 2 of life with 10 8 CFU/ml of a pESI+ or a pESI– S . Infantis strain, respectively. Ten uninfected birds served as in-contact birds to monitor transmission. In both groups, an increase of infection was observed from 7 days of age onwards, reaching its peak at 28 days. However, the pESI+ strain proved significantly more virulent being re-isolated from most cloacal swabs and organs by direct plating. In contrast, the pESI– strain could be re-isolated from cloacal swabs and caeca only when enrichment was applied. Although the excretion of this strain was limited, the transmission level to in-contact birds was similar to the pESI+ strain. Differences in infection dynamics were also reflected in the antibody response: whereas the pESI+ strain provoked a significant increase in antibodies, antibody levels following infection with the pESI– strain remained in the range of negative control birds. The actual findings provide for the first time evidence of S . Infantis strain-specific infectivity in broilers and confirm previous observations in the field regarding differences in persistence on farms and resistance against disinfectants.

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“…In the field of food science, there is also increasing concern among consumers about the use of synthetic preservatives in food, as some of them, such as sulfites and nitrites, have been linked to possible adverse effects on human health [5,6]. There has also been an increase in the resistance of food pathogens to biocides commonly used in the food industry [7]. Therefore, one of the main challenges of actual society is the search for alternative substances to antibiotics and preservatives.…”

Section: Introductionmentioning

confidence: 99%

Milk Microbiota: A Source of Antimicrobial-Producing Bacteria with Potential Application in Food Safety

Lamas¹,

Sanjulián²,

Cepeda³

et al. 2020

The 1st International Electronic Conference on Food Science and Functional Foods

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Antimicrobial and biocide resistance is a major public health problem today. Therefore, one of the main scientific challenges nowadays is the search for alternatives to these substances. One of these potential alternatives are the bacteriocins. Microbiota are a potential source of bacteriocin-producing bacteria that need to be studied. In this study, a total of 40 samples of human milk and 10 samples of cow milk were collected from healthy individuals and stored at −20 °C until use. Colonies isolated from these samples that showed antimicrobial activity against Lactobacillus delbrueckii ssp. bulgaricus in the overlaid assays were selected. Well diffusion assays were carried out with the cell-free supernatant (CFS) from these colonies neutralized to pH and inhibition zones were recorded. The activity against eight common bacterial pathogens was evaluated. A total of 32 colonies with potential antimicrobial activity were isolated. The neutralized CFS of 10 strains showed antimicrobial activity against at least one pathogen tested in the well diffusion assays. Eight of the 10 CFS inhibited the growth of Staphylococcus aureus. These CFS also showed activity against Staphylococcus epidermidis, Streptococcus agalactiae, Pseudomonas aeruginosa , L. monocytogenes, Clostridium perfringens and Clostridioides difficile. The next steps of the research will be 16s rRNA sequencing to identify the species of isolates and mass spectrometry to determine the antimicrobial product produced by isolation. Finally, this study demonstrated that milk microbiota are a potential source of new producing bacteriocin bacteria that can be used in the formulation of new food products.

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In-vitro testing of bacteriostatic and bactericidal efficacy of commercial disinfectants against Salmonella Infantis reveals substantial differences between products and bacterial strains

Cited by 28 publications

References 21 publications

Study on the extraction and antibacterial activity of Monascin

Study on the extraction and antibacterial activity of Monascin

Infection dynamics of Salmonella Infantis strains displaying different genetic backgrounds – with or without pESI-like plasmid – vary considerably

Milk Microbiota: A Source of Antimicrobial-Producing Bacteria with Potential Application in Food Safety

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