In vitro tetraploid induction of Malus × domestica Borkh. using leaf or shoot explants

Podwyszyńska, Małgorzata; Sowik, I.; Machlańska, Aleksandra; Kruczyńska, D.; Dyki, B.

doi:10.1016/j.scienta.2017.08.042

Cited by 29 publications

(23 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When antimitotic treatment is applied to, e.g., leaf tissue, which does not contain any organized meristems, the shoots developing from individual cells via adventitious organogenesis may be diploid or tetraploid. Similarly, as in our study, high numbers of mixoploids were obtained during polyploidisation of shoot explants in, e.g., Humulus lupulus (Trojak-Goluch, Skomra, 2013) and Malus × domestica (Podwyszyńska et al, 2017). In apple, the induction of chromosome doubling using leaf explants resulted in only a few mixoploids, compared to the high numbers of homogenous tetraploids obtained after antimitotic treatments of this type of explants (Podwyszyńska et al, 2017).…”

Section: Discussionsupporting

confidence: 81%

“…Samples (1-2 leaves and shoot base) were taken from all the shoots obtained. Plant tissue was chopped in a Petri dish in 0.5 ml nuclei isolation Partec buffer with 1% polyvinylpyrrolidone (PVP), to which 50 μg ml -1 4',6-diamidino-2-phenylindole (DAPI) was added (Podwyszyńska et al, 2017). After adding 1 ml of the isolation buffer, the samples were filtered through a 30-μm filter and incubated for 45-60 min in the dark at room temperature.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

In vitro tetraploid induction of the blackcurrant (Ribes nigrum L.) and preliminary phenotypic observations

Podwyszyńska

Pluta

2019

Zemdirbyste-Agriculture

Self Cite

View full text Add to dashboard Cite

The aim of this study was to develop an in vitro polyploidisation method for the breeding of new improved cultivars of blackcurrant (Ribes nigrum L.). The Polish blackcurrant cultivars 'Gofert' and 'Polares' were used for the experiments. For polyploidisation, shoot explants were incubated for six days in the dark on a modified Murashige and Skoog medium supplemented with 4.5 µM 6-benzylaminopurine (BAP), 0.3 µM gibberellic acid (GA 3) and 0.5 µM indole-3-acetic acid (IAA), containing one of the following antimitotic agents: colchicine, trifluralin, oryzalin or amiprophos methyl. The strongest phytotoxic effects of antimitotic agents were observed for trifluralin (all the explants died). Tetraploids were obtained for both cultivars: 21 for 'Gofert' and 12 for 'Polares', respectively. The higher efficiencies of tetraploid induction were recorded with oryzalin (5 mg L-1), 26.3% and 28.6% for 'Gofert' and 'Polares', respectively; 25.9% with colchicine (125 mg L-1) for 'Polares' and 9.3% with amiprophos methyl (5 mg L-1) for 'Gofert'. In addition, only six (2.4%) homogeneous 'Gofert' tetraploids were selected directly. Instead, the high percentage (30.1%) of mixoploids was detected in this cultivar. All mixoploid shoots were subcultured twice. An additional 15 homogeneous tetraploids were selected based on the flow cytometry analysis from the mixoploid shoot cultures, but only from among those with a predominance of the tetraploid genotype. The polyploidisation efficiency was on average 8.4% for 'Gofert' and 9.2% for 'Polares'. In vitro growth and shoot multiplication of newly obtained tetraploids were very poor in the presence of BAP. Therefore, in order to obtain tetraploid shoots capable of rooting, shoot multiplication was optimized by application of 6-(3-hydroxybenzylamino)purine (meta-topolin). Replacement of BAP with meta-topolin in the medium allowed faster multiplication of the newly obtained tetraploids, which were then rooted in vitro and grown in a greenhouse. The tetraploids differed phenotypically from their diploid counterparts. Detailed measurements were done for 'Gofert' after six months of growing plants in vitro. Compared to diploid plants, tetraploids had shorter shoots, they contained fewer leaves, their leaf shape was different and chlorophyll index was higher.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

In vitro tetraploid induction of the blackcurrant (Ribes nigrum L.) and preliminary phenotypic observations

Podwyszyńska

Pluta

2019

Zemdirbyste-Agriculture

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tetraploids of above mentioned cultivars were obtained in our previous study using in vitro techniques [Podwyszyńska et al 2017]. Selected tetraploids (two of each cultivar) were cloned to about 30 shoot clumps and such shoot cultures were maintained and served to rooting experiments.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

“…Triploid or tetraploid varieties are characterized by a number of valuable features: larger fruits and often greater resistance to biotic and abiotic stress [Sedysheva and Gorbacheva 2013]. In the Research Institute of Horticulture (Skierniewice, Poland) for breeding purposes, tetraploids of five cultivated apple cultivars were obtained by in vitro mitotic polyploidization [Podwyszyńska et al 2017]. Shoots of the newly obtained tetraploids differed significantly from those of their diploid counterparts.…”

Section: Introductionmentioning

confidence: 99%

“…Zimmerman and Fordham [1985] proved that increased temperature to even 30°C and darkness during the induction phase lasting 3-7 days significantly enhanced shoot rooting frequency. The aim of the study was to develop an effective method of rooting microcuttings of apple tetraploids newly obtained by in vitro technique [Podwyszyńska et al 2017]. Due to the limited amount of tetraploid shoots, in the first stage of this research, the method of in vitro rooting was optimized for shoots of diploid donor cultivars.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Podwyszyńska

Cieślińska

2018

Acta Sci.Pol.Hortorum Cultus

Self Cite

View full text Add to dashboard Cite

For breeding purposes, the number of neo-tetraploids of apple cultivars have been derived by in vitro technique. The first attempts at rooting and acclimatization of tetraploid shoots failed. The aim of the study was to develop an effective method for rooting microcuttings of apple neo-tetraploids. In the first stage of the study, in vitro rooting method was optimized for the shoots of diploid donor cultivars. Shoots were rooted on Murashige and Skoog [1962] (MS) medium with a reduced content of nitrogen, in the presence of auxins alone or in combination (indole-3-butyric acid -IBA, 1-naphthaleneacetic acid -NAA and indole--3-acetic acid -IAA) with addition of putrescine, arginine or ornithine. The compounds were applied continuously for 25 days (one-step rooting system) or for seven days with subsequent transplanting shoots onto a medium without these compounds (two-step rooting method). Tetraploid microcuttings of the cultivars 'Free Redstar', 'Gala Must', 'Pinova' and 'Redchief' were evaluated for rooting on the selected medium considered optimal for their diploid counterparts. The shoots of all diploid apple scion cultivars had low rooting capacity. IBA alone poorly stimulated root formation. Significant improvement of rooting to 60-80% was achieved through the application of auxins, 2.5 µM IBA or 1.3 µM NAA combined with 5 µM IAA and 50 µM putrescine in the two-step rooting system with darkness and increased temperature of 26°C during seven-day induction phase. The replacement of benzyladenine (BA) by meta-Topolin (m-T) in the last multiplication subculture influenced positively shoot acclimatization. Tetraploids had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts.

show abstract

Ploidy, Genome Size, and Cytogenetics of Apple

Podwyszyńska

Marasek-Ciołakowska

2021

Compendium of Plant Genomes

View full text Add to dashboard Cite

In vitro tetraploid induction of Malus × domestica Borkh. using leaf or shoot explants

Cited by 29 publications

References 40 publications

In vitro tetraploid induction of the blackcurrant (Ribes nigrum L.) and preliminary phenotypic observations

In vitro tetraploid induction of the blackcurrant (Ribes nigrum L.) and preliminary phenotypic observations

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Ploidy, Genome Size, and Cytogenetics of Apple

Contact Info

Product

Resources

About