In vitro thymidine labelling of human pulmonary neoplasms

Kerr, K.M.; Robertson, Aileen; Lamb, D

doi:10.1038/bjc.1983.32

Cited by 18 publications

(17 citation statements)

References 15 publications

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“…Published data for the length of the S-phase (TJ) in human tumours suggests that assuming T, to be 15 h is reasonably justified for the cases in this study (Frindel et al, 1968;Terz et al, 1971;Muggia et al, 1974;Straus & Moran, 1977;Rabes et al, 1979). Comparative calculations of DTpot shown in (Kerr et al, 1983). In any case the errors estimating DTpot in this way are not even of the appropriate order of magnitude to account for the large differences (often a factor of between 10 and 100) between DTpot and DT.…”

Section: Discussionsupporting

confidence: 51%

“…Is it that cell replication carries with it a certain "casualty rate" or that increased expansion rate in a cell population means too great a demand on nutrient supply leading to cell death (Malaise et al, 1973)? Of relevance in this context is the finding that increased cell loss makes tumour response to chemotherapy more likely (Bagshawe, 1968); this being especially so, as we might expect, in tumours with a high TLI (Tubiana & Malaise, 1975 (Kerr et al, 1983) to other groups'. Thus mean 0 in small cell cancer as a group may be less than in other types of bronchogenic carcinoma.…”

Section: Discussionmentioning

confidence: 92%

“…We have already reported a series of 58 human pulmonary neoplasms on which TLIs were determined using an in vitro technique (Kerr et al, 1983). All 57 patients (one patient had two metastases resected) had carcinomata removed at thoracotomy in the Thoracic Surgical Unit, City Hospital, Edinburgh.…”

Section: Methodsmentioning

confidence: 99%

“…() The Macmillan Press Ltd., 1984 Histological classification of lung tumours The tumours were classified histologically on the material taken from the resected specimen for diagnostic purposes according to a modification of the WHO classification of lung tumours previously used by us (Kerr et al, 1983).…”

Section: Methodsmentioning

confidence: 99%

“…The former group consisted of 5 squamous carcinomata, 3 large cell undifferentiated carcinomata (2 with and one without stratification), 3 (Kerr et al, 1983 (Kerr et al, 1983).…”

Section: Methodsmentioning

confidence: 99%

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Actual growth rate and tumour cell proliferation in human pulmonary neoplasms

Kerr¹,

Lamb²

1984

Br J Cancer

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The former group consisted of 5 squamous carcinomata, 3 large cell undifferentiated carcinomata (2 with and one without stratification), 3 (Kerr et al, 1983 (Kerr et al, 1983).…”

Section: Methodsmentioning

confidence: 99%

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Actual growth rate and tumour cell proliferation in human pulmonary neoplasms

Kerr¹,

Lamb²

1984

Br J Cancer

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The expression of proliferating cell nuclear antigen in paraffin sections of peripheral, node-negative non-small cell lung cancer

Fontanini

Macchiarini

Pepe

et al. 1992

Cancer

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Cell proliferation of 40 peripheral, node‐negative non‐small cell lung cancers (NSCLC) treated with surgery alone was investigated by immunohistochemical analysis with the monoclonal antibody (MoAb) PC10, which recognizes a proliferating cell nuclear antigen (PCNA) in formalin‐fixed and paraffin‐embedded material. Results were correlated with DNA ploidy and S‐phase fraction (SPF) analyzed by DNA flow cytometric study. Mitotic count (MC) was analyzed by light microscopic study and histopathologic features. PCNA immunoreactivity was seen in all samples and confined to the nuclei of cancer, but not to the surrounding, tumor‐negative cells; its frequency ranged from 0–70% (median, 15%), and tumors expressed either a low (0–25%, n = 25) or intermediate (26–75%, n = 15) proliferative activity. There was no relationship between PCNA immunoreactivity and tumor stage or among size, histologic type, and mitotic count (MC). Tumors with intratumoral blood vessel invasion (BVI) showed a significantly higher (P < 0.005) PCNA immunoreactivity than BVI‐negative tumors. PCNA scores were significantly higher (P < 0.005) in DNA aneuploid (n = 22) than in DNA diploid (n = 18) tumors and correlated significantly with the SPF of DNA aneuploid tumors (r = 0.825, P < 0.0001), but not with diploid tumors (r = 0.002, P = < 0.9). Intermediate proliferating tumors had a significantly higher (P < 0.01) MC than their counterparts. In univariate analysis, significant predictors of survival were tumor classification (T1 versus T2), tumor size (less than or equal to 2.6 cm versus more than 2.6 cm), BVI (BVI‐negative versus BVI‐positive), MC (less than or equal to 8 versus more than 8), and PCNA immunoreactivity (low versus intermediate). DNA ploidy and SPF did not influence survival significantly. Only PCNA immunoreactivity retained its independent level of significance (P = 0.02) by multivariate analysis. It was concluded that PCNA immunostaining is a simple and clinically useful method for estimating cell proliferation in formalin‐fixed, paraffin‐embedded tissue of resected peripheral, node‐negative NSCLC.

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Flash labelling of S‐phase cells in short‐term organ culture of normal and pathological human endometrium using bromodeoxyuridine and tritiated thymidine

Thornton

Wells

Hume

1988

The Journal of Pathology

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Normal hyperplastic and malignant endometrial specimens were labelled in vitro with bromodeoxyuridine (BrdU). S-phase cells were stained after DNA denaturation, using a monoclonal antibody to BrdU and an indirect immunoperoxidase method. Various conditions for BrdU uptake, DNA denaturation, and staining were tested. BrdU labelling was compared with autoradiography using tritiated thymidine, with good correlation. Glandular labelling indices of proliferative endometrium were significantly higher than both secretory and hyperplastic endometrium but were similar to carcinoma. Stromal labelling showed the same trend but the differences were not statistically significant.

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In vitro thymidine labelling of human pulmonary neoplasms

Abstract: SummaryThe in vitro thymidine labelling indices (TLI) of 58 human lung tumours were assessed using autoradiography. The

Cited by 18 publications

References 15 publications

Actual growth rate and tumour cell proliferation in human pulmonary neoplasms

Actual growth rate and tumour cell proliferation in human pulmonary neoplasms

The expression of proliferating cell nuclear antigen in paraffin sections of peripheral, node-negative non-small cell lung cancer

Flash labelling of S‐phase cells in short‐term organ culture of normal and pathological human endometrium using bromodeoxyuridine and tritiated thymidine

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