In vitro toxicity of 1,2-dibromo-3-chloropropane (DBCP) in different testicular cell types from rats

Bjørge, Christine

doi:10.1016/0890-6238(95)00038-c

Cited by 26 publications

(14 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few specific markers are available, and none alone has the power to identify the stage [41][42][43][44]. Staining for CD46 provides an acrosome marker that, when combined with DAPI staining and morphological parameters (bundling and position in profile), enables accurate staging.…”

Section: Discussionmentioning

confidence: 99%

Expression of CD46 in Developing Rat Spermatozoa: Ultrastructural Localization and Utility as a Marker of the Various Stages of the Seminiferous Tubuli1

Mizuno

Harris

Suzuki

et al. 2005

Biology of Reproduction

View full text Add to dashboard Cite

Identification of the various stages of the seminal tubule epithelium that are important in spermatogenesis in humans and rodents requires considerable expertise for analysis of ultrastructural appearance under light microscopy. Few good stage-specific markers have been reported to facilitate the process. We recently described characterization of the expression of CD46 (membrane cofactor protein) in the rat using a novel monoclonal antibody. Expression of CD46 was restricted to spermatozoa and their immediate precursors in the testis. In the present study, we used a combination of morphological analyses, known acrosome markers, actin staining, direct nuclear staining, and staining for CD46 to delineate precisely the subcellular location of CD46. Staining of CD46 colocalized with known acrosome markers in late spermatids and mature spermatozoa and was confirmed by electron microscopy to be acrosome-restricted. Expression was first detected in step 7 spermatids, whereas known markers were not expressed until step 9. The CD46 staining pattern differed through spermatid development, and distinct patterns of staining could be identified that, when combined with 4'-6-diamino-2-phenylindole-2HCl nuclear staining, enabled the accurate staging of the seminiferous tubule epithelium in different profiles. This detailed description of the spatiotemporal expression patterns of CD46 provides a valuable tool for analysis of spermatogenesis in the rat. Furthermore, this information will aid ongoing studies regarding the roles of CD46 in acrosome-related spermatozoal functions.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of CD46 in Developing Rat Spermatozoa: Ultrastructural Localization and Utility as a Marker of the Various Stages of the Seminiferous Tubuli1

Mizuno

Harris

Suzuki

et al. 2005

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…[521 [531 [54] [55] endemic organisms such as earthworms 301 and plant cells 31) will be suitable to detect the genotoxic effects of pollutants in the soil and in the plant systems (Table 1).…”

Section: The Methodsmentioning

confidence: 99%

Single-cell gel electrophoresis (SCG)-A review and discussion

Takeshita

Morimoto

1997

Environ Health Prev Med

View full text Add to dashboard Cite

Single-cell gel electrophoresis (SCG) is a simple, sensitive and effective technique. Being able to reflect quantitatively the genotoxicity of many hazardous agents, it is promising for application in environmental genotoxic monitoring and the study of carcinogenesis. In clinics, it can be used to evaluate the DNA repair ability and monitor DNA breaks during cancer therapy. As a biomarker, it has its own merits and limitations, being different from other biomarkers such as sister chromatid exchange (SCE) test and micronudei (MN) assay. In many studies, it is more sensitive than SCE or MN.Combination studies with other biomarkers like SCE, MN, chromosomal aberration, bcl-2 and genetic polymorphisms have begun to demonstrate its great importance for the understanding of carcinogenesis and the genotoxicities of environmental factors.

show abstract

“…This revealed the presence of DNA strand-breaks in non-treated cells, whose numbers decreased with the duration of the culture, suggesting the involvement of DNA repair mechanisms related to meiotic recombination (Perrin et al 2007). Besides repair capacity, it should also be taken into account that when using cells from testes for in vitro studies, various testicular cell types show differences in metabolic activation of chemical compounds (Bjorge et al 1995). Anderson et al (1997c) believe that there are low levels of metabolic activity even in sperm because the heterocyclic amines normally requiring metabolic activation have shown positive responses.…”

Section: In Vitro Comet Assay With Spermmentioning

confidence: 99%

The comet assay in male reproductive toxicology

2007

View full text Add to dashboard Cite

Due to our lifestyle and the environment we live in, we are constantly confronted with genotoxic or potentially genotoxic compounds. These toxins can cause DNA damage to our cells, leading to an increase in mutations. Sometimes such mutations could give rise to cancer in somatic cells. However, when germ cells are affected, then the damage could also have an effect on the next and successive generations. A rapid, sensitive and reliable method to detect DNA damage and assess the integrity of the genome within single cells is that of the comet or single-cell gel electrophoresis assay. The present communication gives an overview of the use of the comet assay utilising sperm or testicular cells in reproductive toxicology. This includes consideration of damage assessed by protocol modification, cryopreservation vs the use of fresh sperm, viability and statistics. It further focuses on in vivo and in vitro comet assay studies with sperm and a comparison of this assay with other assays measuring germ cell genotoxicity. As most of the de novo structural aberrations occur in sperm and spermatogenesis is functional from puberty to old age, whereas female germ cells are more complicated to obtain, the examination of male germ cells seems to be an easier and logical choice for research and testing in reproductive toxicology. In addition, the importance of such an assay for the paternal impact of genetic damage in offspring is undisputed. As there is a growing interest in the evaluation of genotoxins in male germ cells, the comet assay allows in vitro and in vivo assessments of various environmental and lifestyle genotoxins to be reliably determined.

show abstract

In vitro toxicity of 1,2-dibromo-3-chloropropane (DBCP) in different testicular cell types from rats

Cited by 26 publications

References 44 publications

Expression of CD46 in Developing Rat Spermatozoa: Ultrastructural Localization and Utility as a Marker of the Various Stages of the Seminiferous Tubuli1

Expression of CD46 in Developing Rat Spermatozoa: Ultrastructural Localization and Utility as a Marker of the Various Stages of the Seminiferous Tubuli1

Single-cell gel electrophoresis (SCG)-A review and discussion

The comet assay in male reproductive toxicology

Contact Info

Product

Resources

About