In Vitro Transcription of Plant Nuclear Genes

Schweizer, Patrick

doi:10.1007/978-3-540-48037-2_5

Cited by 2 publications

(2 citation statements)

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“…Our results furthermore suggest a low degree of sequence similarity between plant Inr's, as has been found for animal Inr's [37]. Nevertheless, some similarity between the CHS Inr and the cap-site sequences of the CaMV 19S promoter [5], the TC7 promoter [56] and the bean CHS15 promoter [1], from which in vitro transcription has been reported, exists with a tendency for a high proportion of A and C residues around the initiation site [46]. TATAbox-independent, Inr-directed in vitro transcription was also found in a rice nuclear extract [36].…”

Section: Discussionmentioning

confidence: 54%

Initiator-dependent transcription in vitro by a wheat germ chromatin extract

Schweizer¹,

Mösinger²

1994

Plant Mol Biol

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The development of plant in vitro transcription systems transcribing faithfully and efficiently from a broad range of plant nuclear promoters has remained a challenge. We examined the nucleotide sequence requirements for faithful and efficient transcription in a wheat germ chromatin extract (Yamazaki et al., Plant Mol Biol Rep 8: 114-123). The wheat germ chromatin extract was tested with a series of chimeric promoter constructs containing plant promoter sequences upstream from the TATA box, TATA boxes, and cap-site sequences (from -10 to +14, relative to the major in vivo initiation site) in different combinations. The plant extract transcribed faithfully from several chimeric promoters containing the capsite sequence of the parsley chalcone synthase promoter. The transcription was sensitive to the RNA polymerase II-specific inhibitor alpha-amanitin and was only dependent on the chalcone synthase cap-site sequence which therefore fulfils the operational criteria for a plant initiator element. Mutations of the putative chalcone synthase initiator element defined a core sequence '5'TAACAAC' around the initiation site that was necessary for efficient transcription in vitro. In contrast to the extract, purified wheat germ RNA polymerase II showed no preference for transcription from the major chalcone synthase in vivo initiation site.

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Section: Discussionmentioning

confidence: 54%

Initiator-dependent transcription in vitro by a wheat germ chromatin extract

Schweizer¹,

Mösinger²

1994

Plant Mol Biol

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“…These systems can be useful for studying the role of protein-protein interaction and the possible involvement of low-molecular mass substances in transcription. Therefore, the availability of versatile in vitro transcription systems from plant cells has long been awaited (32,37,47).…”

Section: Introductionmentioning

confidence: 99%

Plant in Vitro Transcription Systems

Sugiura

1997

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

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In vitro transcription systems provide a powerful tool for detailed analysis of transcription reactions including initiation, elongation, and termination. Despite problems inherent to plant cells, efforts have been made to develop plant in vitro transcription systems in the past decade. These efforts have finally culminated in the development of reliable in vitro systems from suspension-cell cultures of both monocot and dicot plants. These systems can be useful in elucidating the specific mechanisms involved in the process of plant transcription and thus can potentially open a new era of transcription studies in plants.

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In Vitro Transcription of Plant Nuclear Genes

Cited by 2 publications

References 95 publications

Initiator-dependent transcription in vitro by a wheat germ chromatin extract

Initiator-dependent transcription in vitro by a wheat germ chromatin extract

Plant in Vitro Transcription Systems

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