In vitro transdifferentiation of human cultured CD34+ stem cells into oligodendrocyte precursors using thyroid hormones

All Wrapped Up: Environmental Effects on Myelination

“…In summary, neural circuit plasticity is a dynamic sum of its moving parts - each component synchronously adapting to augment circuit function and ultimately behavior. Dysregulation of the oligodendroglial/myelin component has significant clinical ramifications throughout the human lifespan [35,36], but the potential of modulating myelogenic cells to influence behavior, cognition, and neural regeneration is an exciting clinical prospect.…”

Effect of 4-methoxy 1-methyl 2-oxopyridine 3-carbamide onStaphylococcus aureusby inhibiting UDP-MurNAc-pentapeptide, peptidyl deformylase and uridine monophosphate kinase

“…The total RNA was isolated from the bacteria using MEDOX-Easy ™ Spin column total RNA Minipreps Kit and cDNA was synthesized using high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). Reverse transcription was carried out in a thermocycler calibrated at 25°C for 10 min, 37°C for 120 min and 85°C for 5 min and thus the cDNA obtained was used for quantitative real-time PCR Venkatesh et al 2015).…”

“…The expression of PDF and MurF genes was quantified using DNA gyrase expression as an endogenous control (Table 1), with penicillin and ampicillin as standard. The expression levels were calculated using the formula DDC T = DC Tr (culture treated with the drug)ÀDC T (untreated control) and relative expression was calculated using N = 2 ÀDDCT (Livak and Schmittgen 2001;Srikanth et al 2015;Venkatesh et al 2015).…”

Mesenchymal cells of umbilical cord and umbilical cord blood as a source of human oligodendrocyte progenitors

“…Nonetheless, the progress in oligodendrocyte generation from WJ-MSC has been made and the cells were successfully used in cellular therapy in mice model of multiple sclerosis[Mikaeili Agah et al, 2014].Given the above considerations, the results obtained in our (Fig.2C-E) and other laboratories show that the process of oligodendrocyte generation in vitro[Sypecka et al, 2009;Venkatesh et al, 2015] and promotion of myelinogenesis in vivo[Marziali et al, 2015;Ramos-Cejudo et al, 2015] could be modulated by application of physiological compounds engaged in natural regulation of oligodendrogenesis. Another aspect of planned oligodendrocyte progenitor transplantation which should be taken into account is the local microenvironment of the nervous tissue that guides transplanted cell differentiation[Moore et al, 2015;Sypecka et al, 2013.…”