In Vitro translation of cucumoviral satellites I. Purification and nucleotide sequence of cucumber mosaic virus-associated RNA 5 from cucumber mosaic virus strain S

The 368-Nucleotide Satellite of Cucumber Mosaic Virus Strain Y from Japan Does Not Cause Lethal Necrosis in Tomato

“…Similar massive differences from Y-CARNA 5 can be observed in the corresponding regions of the satellite RNAs of CMV strains 1, Q and S, while the remainder of their sequences, like D-CARNA 5, have retained more than 90~ sequence homology (Avila-Rincon et al, 1986).…”

“…That such far-reaching changes in part of the structure of D-CARNA 5 do not affect the necrogenic capability of this molecule, whereas much lesser differences, as in the CARNA 5 species of CMV strains 1, Q and S, do (Avila-Rincon et al, 1986), seems contradictory. Also, assigning the property of tomato necrosis induction to specific satellites requires considerable caution, and perhaps a more precise definition of the term.…”

“…Also, assigning the property of tomato necrosis induction to specific satellites requires considerable caution, and perhaps a more precise definition of the term. Frequently, two or more CARNA 5 variants may coexist in a given CMV preparation (Kaper, 1983;Garcia-Luque et al, 1984;Avila-Rincon et al, 1986), which could result in an incorrect assignment of a biological property belonging to a CARNA 5 species present in minor proportion in the mixture. Defining tomato necrosis as the disease where, under well specified inoculation conditions of Rutgers tomato plants (Lycopersicon esculentum Mill cv.…”

The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs

“…Other early work (Kaper & Tousignant, 1977) showed its failure to support the accumulation of satellite RNA isolated from CMV-S. The satellite isolate used in that work turned out to be a mixture of both a necrogenic and a non-necrogenic satellite RNA (Avila- Rincon et al, 1986). Subsequent research showed that CMV-Ix could support the accumulation of a necrogenic satellite T-CARNA 5, albeit without the induction of tomato necrosis, but not the prototype necrogenic satellite D-CARNA 5, although it shares 97 % sequence identity with T-CARNA 5 (Kaper et al, 1990b).…”

In vitro messenger properties of a satellite RNA of cucumber mosaic virus

“…Cucumber mosaic virus (CMV) satellite RNA (satRNA) has been isolated from various strains of CMV, and the nucleotide sequences of 22 variants are known (Richards et al, 1978;Gordon & Symons, 1983;Collmer et al, 1983;Hidaka et al, 1984Hidaka et al, , 1988Avila-Rincon et al, 1986a;Kaper et al, 1986Kaper et al, , 1988Garcia-Arenal et al, 1987;Jacquemond & Lauquin, 1988). The molecular size of satRNA was similar in 20 variants (333 to 342 nucleotides), whereas the other two were larger (368 and 386 nucleotides respectively).…”