In-vitro-Untersuchung der Morphologie der Harnblasenwand in der MR-Tomographie bei 1,0 Tesla: Korrelation mit der Histologie

Teufl, F.; Dammann, Florian; Wehrmann, Manfred

doi:10.1055/s-2007-1015449

Cited by 11 publications

(5 citation statements)

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“…These, in turn, are hardly possible to conduct on intact human bladder tissue. Alternatively, the examination of pig bladder strips is of special importance due to the structural and mechanical similarities shared with the human bladder (Teufl et al, 1997; Dahms et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Locational and Directional Dependencies of Smooth Muscle Properties in Pig Urinary Bladder

et al. 2019

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The urinary bladder is a distensible hollow muscular organ, which allows huge changes in size during absorption, storage and micturition. Pathological alterations of biomechanical properties can lead to bladder dysfunction and loss in quality of life. To understand and treat bladder diseases, the mechanisms of the healthy urinary bladder need to be determined. Thus, a series of studies focused on the detrusor muscle, a layer of urinary bladder made of smooth muscle fibers arranged in longitudinal and circumferential orientation. However, little is known about whether its active muscle properties differ depending on location and direction. This study aimed to investigate the porcine bladder for heterogeneous (six different locations) and anisotropic (longitudinal vs. circumferential) contractile properties including the force-length-(FLR) and force-velocity-relationship (FVR). Therefore, smooth muscle tissue strips with longitudinal and circumferential direction have been prepared from different bladder locations (apex dorsal, apex ventral, body dorsal, body ventral, trigone dorsal, trigone ventral). FLR and FVR have been determined by a series of isometric and isotonic contractions. Additionally, histological analyses were conducted to determine smooth muscle content and fiber orientation. Mechanical and histological examinations were carried out on 94 and 36 samples, respectively. The results showed that maximum active stress (pact) of the bladder strips was higher in the longitudinal compared to the circumferential direction. This is in line with our histological investigation showing a higher smooth muscle content in the bladder strips in the longitudinal direction. However, normalization of maximum strip force by the cross-sectional area (CSA) of smooth muscle fibers yielded similar smooth muscle maximum stresses (165.4 ± 29.6 kPa), independent of strip direction. Active muscle properties (FLR, FVR) showed no locational differences. The trigone exhibited higher passive stress (ppass) than the body. Moreover, the bladder exhibited greater ppass in the longitudinal than circumferential direction which might be attributed to its microstructure (more longitudinal arrangement of muscle fibers). This study provides a valuable dataset for the development of constitutive computational models of the healthy urinary bladder. These models are relevant from a medical standpoint, as they contribute to the basic understanding of the function of the bladder in health and disease.

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Section: Introductionmentioning

confidence: 99%

Locational and Directional Dependencies of Smooth Muscle Properties in Pig Urinary Bladder

et al. 2019

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“…Extrapolating findings generated using small laboratory animals to the physiology of urine storage and voiding in large animals and the clinical context is difficult, and requires validation as additional issues have to be reconciled such as: differences in the base-line physiological properties of larger bladders and experimental considerations (adequate tissue perfusion and environmental control). Studies have evaluated the urodynamic properties of pig in vivo , demonstrating comparable urodynamic (Sibley, 1984; Crowe and Burnstock, 1989; Mills et al, 2000b; Moore and Brading, 2007) and structural (Dixon and Gosling, 1983; Teufl et al, 1997) characteristics to humans, and potential clinical relevance. A novel extracorporeal perfusion model using isolated pig urinary bladder has been used to study its contractile properties (Dittrich et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

The validation of a functional, isolated pig bladder model for physiological experimentation

Parsons

Drake²,

Gammie

et al. 2012

Front. Pharmacol.

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Characterizing the integrative physiology of the bladder requires whole organ preparations. The purpose of this study was to validate an isolated large animal (pig) bladder preparation, through arterial and intravesical drug administration, intravesical pressure recording, and filming of surface micromotions. Female pig bladders were obtained from the local abattoir and arterially perfused in vitro. Arterial and intravesical pressures were recorded at varying volumes. Bladder viability was assessed histologically and by monitoring inflow and outflow pH. Arterial drug administration employed boluses introduced into the perfusate. Intravesical administration involved slow instillation and a prolonged dwell-time. Surface micromotions were recorded by filming the separation of surface markers concurrently with intravesical pressure measurement. Adequate perfusion to all bladder layers was achieved for up to 8 h; there was no structural deterioration nor alteration in inflow and effluent perfusate pH. Arterial drug administration (carbachol and potassium chloride) showed consistent dose-dependent responses. Localized movements (micromotions) occurred over the bladder surface, with variable correlation with fluctuations of intravesical pressure. The isolated pig bladder is a valid approach to study integrative bladder physiology. It remains viable when perfused in vitro, responds to different routes of drug administration and provides a model to correlate movements of the bladder wall directly to variation of intravesical pressure.

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“…Though the human bladder wall is more distinguishable in T 2 -weighted MRI ( Figure 1D ) 63 , 73 , T 1 -weighted MRI 67 is preferable for its higher signal-to-noise ratio (SNR) and accurate measurement of BWT 69 , 73 – 75 for characterizing bladder disorders 51 . As shown in Figure 1 , the thickness of the inner layer (urothelium) of the bladder wall is underestimated in T 2 -weighted images 75 , 76 . The bladder wall appears as a thin layer of intermediate intensity on T 2 -weighted images and is likely to correspond to the thickness indicated by of the middle and outer layer on the contrast-enhanced T 1 -weighted image ( Figure 1C ) 65 .…”

Section: Imaging Techniquesmentioning

confidence: 91%

Recent advances in imaging and understanding interstitial cystitis

et al. 2018

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Interstitial cystitis/bladder pain syndrome (IC/BPS) is a debilitating condition associated with intense pelvic pain and bladder storage symptoms. Since diagnosis is difficult, prevalence estimates vary with the methodology used. There is also a lack of proven imaging tools and biomarkers to assist in differentiation of IC/BPS from other urinary disorders (overactive bladder, vulvodynia, endometriosis, and prostatitis). Current uncertainty regarding the etiology and pathology of IC/BPS ultimately impacts its timely and successful treatment, as well as hampers future drug development. This review will cover recent developments in imaging methods, such as magnetic resonance imaging, that advance the understanding of IC/BPS and guide drug development.

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In-vitro-Untersuchung der Morphologie der Harnblasenwand in der MR-Tomographie bei 1,0 Tesla: Korrelation mit der Histologie

Abstract: MR imaging enables the differentiation of three bladder wall layers. Inversion recovery technique achieved the best image quality by avoiding chemical shift artifact.

Cited by 11 publications

References 0 publications

Locational and Directional Dependencies of Smooth Muscle Properties in Pig Urinary Bladder

Locational and Directional Dependencies of Smooth Muscle Properties in Pig Urinary Bladder

The validation of a functional, isolated pig bladder model for physiological experimentation

Recent advances in imaging and understanding interstitial cystitis

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