In vitro wound healing assays – state of the art

Stamm, Anne; Reimers, Kerstin; Strauß, Sarah; Vogt, Peter M.; Scheper, Thomas; Pepelanova, Iliyana

doi:10.1515/bnm-2016-0002

Cited by 114 publications

(121 citation statements)

References 38 publications

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“…Unlike migration assays, wound-healing assays usually imply a form of cell injury that results in a cell depleted area 94 . In a 2D assay, this is usually obtained by letting cells reach confluence and injure the monolayer through various ways: scratching, stamping, thermal or electric wounding or laser ablation.…”

Section: Wound-healing Assaysmentioning

confidence: 99%

“…Although less used than the tumor spheroid model, wound-healing assays have been developed for studying collective cell behavior in 3D in vitro. The constructs used in 3D wound healing assays usually consist of layered structures, combining ECM components such as collagen hydrogels and cells 94 . Topman et al used such an assay with a transparent hyaluronic-acid based hydrogel to calculate the migration rate and directionality of DAPI-stained cells in vitro 107 .…”

Section: Wound-healing Assaysmentioning

confidence: 99%

See 1 more Smart Citation

Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods

Spatarelu

Zhang

Nguyen

et al. 2019

ACS Biomater. Sci. Eng.

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Cell migration is essential for regulating many biological processes in physiological or pathological conditions, including embryonic development and cancer invasion. In vitro and in silico studies suggest that collective cell migration is associated with some biomechanical particularities, such as restructuring of extracellular matrix (ECM), stress and force distribution profiles, and reorganization of cytoskeleton. Therefore, the phenomenon could be understood by an in-depth study of cells' behavior determinants, including but not limited to mechanical cues from the environment and from fellow "travelers". This review article aims to cover the recent development of experimental and computational methods for studying the biomechanics of collective cell migration during cancer progression and invasion. We also summarized the tested hypotheses regarding the mechanism underlying collective cell migration enabled by these methods. Together, the paper enables a broad overview on the methods and tools currently available to unravel the biophysical mechanisms pertinent to cell collective migration, as well as providing perspectives on future development towards eventually deciphering the key mechanisms behind the most lethal feature of cancer. I.

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Section: Wound-healing Assaysmentioning

confidence: 99%

Section: Wound-healing Assaysmentioning

confidence: 99%

Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods

Spatarelu

Zhang

Nguyen

et al. 2019

ACS Biomater. Sci. Eng.

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Section: Skin Cell Cultures For Wound Healingmentioning

confidence: 99%

“…Both 2D and 3D skin cell cultures are available as wound healing models [60]. 2D wound healing models involve the creation of a site of injury in a monolayer of skin cells, either through mechanical or chemical means, in which cells then migrate to the site of injury [60,61]. Cells in 2D monolayer cultures are thought to adhere to the flat environments, such as a Petri dish, in which they are cultured and will therefore migrate to areas of free space within the dish, an activity thought to mimic in vivo migration involved in cell differentiation [62].…”

Section: Skin Cell Cultures For Wound Healingmentioning

confidence: 99%

2D vs. 3D Cell Culture Models for In Vitro Topical (Dermatological) Medication Testing

Teimouri¹,

Yeung²,

Agu³

2019

Cell Culture

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Due to ethical concerns regarding animal testing, alternative methods have been in development to test the efficacy and safety of pharmaceutical products and medications, specifically topical (dermatological) medications. Two-dimensional (2D) and three-dimensional (3D) skin cell cultures are examples of in vitro methods used as an alternative to animal testing. The first skin cells cultured were keratinocytes, a type of cell predominantly in the epidermal layer of the skin. However, with differences in skin characteristics and pathophysiology of different skin conditions, various skin cell cultures and models to better mimic these differences have been developed. These cell cultures include not only keratinocytes but also other skin cell types, such as fibroblasts, which are predominantly in the dermal layer of the skin, and certain immune cells and even melanocytes. To have a better understanding of the type of cell cultures used for testing dermatological products, this chapter aims to outline the differences between 2D and 3D skin cell cultures while considering the advantages and disadvantages of each culture. Different types of cell culture models used for wound healing and for inflammatory skin conditions such as psoriasis will also be discussed.

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“…However, the important three-dimensional cell and matrix structures are missing in these models. The development of 3D skin models is therefore important progress for wound healing studies [2]. The mechanical wounding of novel 3D models is still an insufficiently outlined topic.…”

Section: Introductionmentioning

confidence: 99%

Image acquisition and planimetry systems to develop wounding techniques in 3D wound model

Kiefer

Parente

Hensler

et al. 2017

Current Directions in Biomedical Engineering

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Wound healing represents a complex biological repair process. Established 2D monolayers and wounding techniques investigate cell migration, but do not represent coordinated multi-cellular systems. We aim to use wound surface area measurements obtained from image acquisition and planimetry systems to establish our wounding technique and in vitro organotypic tissue. These systems will be used in our future wound healing treatment studies to assess the rate of wound closure in response to wound healing treatment with light therapy (photobiomodulation). The image acquisition and planimetry systems were developed, calibrated, and verified to measure wound surface area in vitro. The system consists of a recording system (Sony DSC HX60, 20.4 M Pixel, 1/2.3'' CMOS sensor) and calibrated with 1mm scale paper. Macro photography with an optical zoom magnification of 2:1 achieves sufficient resolution to evaluate the 3mm wound size and healing growth. The camera system was leveled with an aluminum construction to ensure constant distance and orientation of the images. The JPG-format images were processed with a planimetry system in MATLAB. Edge detection enables definition of the wounded area. Wound area can be calculated with surface integrals. To separate the wounded area from the background, the image was filtered in several steps. Agar models, injured through several test persons with different levels of experience, were used as pilot data to test the planimetry software. These image acquisition and planimetry systems support the development of our wound healing research. The reproducibility of our wounding technique can be assessed by the variability in initial wound surface area. Also, wound healing treatment effects can be assessed by the change in rate of wound closure. These techniques represent the foundations of our wound model, wounding technique, and analysis systems in our ongoing studies in wound healing and therapy.

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In vitro wound healing assays – state of the art

Cited by 114 publications

References 38 publications

Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods

Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods

2D vs. 3D Cell Culture Models for In Vitro Topical (Dermatological) Medication Testing

Image acquisition and planimetry systems to develop wounding techniques in 3D wound model

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