In vitro α-Amylase and α-Glucosidase inhibitory activity of extracts of Feronia elephantum fruit and Paspalum scrobiculatum Grains.

Reddy, Gangavaram Jyothi; Reddy, K. N.; Reddy, G. Venkata

doi:10.31024/ajpp.2019.5.s1.4

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…This effect could be related to the inhibitory action on carbohydrate metabolizing enzymes like -glucosidase and pancreatic -amylase as reported previously in our in vitro study. 8 HFD tends to increase body weight as evident from the the present results however no significant change was observed post-STZ administration in all groups. The possible intervention of STZ in lowering body weight should be considered.…”

Section: Discussionsupporting

confidence: 50%

“…Ripe fruits of F. elephantum were collected and processed as described earlier. 8 The plant material was authenticated by Dr. K. Madhava Chetty, Department of Botany, Sri Venkateswara University (SVU) and voucher specimens (Voucher number:1328) were deposited at department of Botany, Sri Venkateswara University (SVU), Tirupati, India. F. elephantum fruits powder was extracted with methanol by soxhlation, filtered, concentrated using Rotavapor (Buchi R-200) and dried in incubator (37°C).…”

Section: Collection Of Plant Materials and Preparation Of Fractionsmentioning

confidence: 99%

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In vivo Anti-diabetic and Anti-hyperlipidemic Activities of ethyl Acetate/Methanol Fractions of Feronia elephantum Fruit in type 2 Diabetic Rats: Via α-amylase and PPAR-γ by using in silico Approach

Reddy¹,

Reddy²,

Reddy³

2020

IJPER

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Background: Obesity and overweight, together with physical inactivity, are projected to cause a huge proportion of the global diabetes burden. Natural products play a key role in the discovery of novel therapeutic agents. Aim: The present study aimed to elucidate in silico and in vivo anti-diabetic and anti-hyperlipidemic potential of Ethyl acetate (EFE) and ethyl acetate:methanol (EMFE) fractions of F. elephantum fruit in diabetic rats. Methods: Male Sprague-Dawley rats were induced type 2 diabetes by high fat diet (HFD) for a period of 2 weeks and then injected intraperitoneally with Streptozotocin (STZ, 35 mg/ kg b.w). Results: Significant antihyperglycemia was displayed in rats treated with EFE and EMFE in OGTT in normal rats. Daily oral treatment with EFE and EMFE each at 100 and 200 mg/kg b.w for 5 weeks resulted in significant reduction in plasma glucose levels and HbA 1c , increased plasma insulin levels, normalised lipid profile and liver function parameters in HFD and STZ model. In addition, degenerative histopathological changes were found to be normalized. Molecular docking studies revealed strong binding efficacy of the active constituents of the extract viz., 22,23-dihydrostigmasterol, tocopherol and campesterol with α-amylase enzyme and PPAR-γ. Conclusion: These results suggest a promising anti-diabetic and anti-hyperlipidemic effect of Feronia elephantum fruit without apparent toxic effects.

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Section: Discussionsupporting

confidence: 50%

Section: Collection Of Plant Materials and Preparation Of Fractionsmentioning

confidence: 99%

In vivo Anti-diabetic and Anti-hyperlipidemic Activities of ethyl Acetate/Methanol Fractions of Feronia elephantum Fruit in type 2 Diabetic Rats: Via α-amylase and PPAR-γ by using in silico Approach

Reddy¹,

Reddy²,

Reddy³

2020

IJPER

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“…According to a previous study, The ethanolic extracts of P. miliaceum and S. italicahave shown α amylase inhibitory action and they reflect the effect of the phenolic compound on that inhibition potential 24 . Reddy and others investigated α amylase inhibition activity of P. scrobiculatum and reported it has remarkable inhibition activity [25] . However, supporting information for the present study's data could not found due to a lack of literature that discussing the α amylase inhibition and amyloglucosidase inhibition activities in terms of dietary fiber content in selected food crops.…”

Section: Resultsmentioning

confidence: 99%

in vitro α amylase and amyloglucosidase inhibitory activities of selected underutilized cereals, yams and root crops

Chiranthika¹,

Chandrasekara²,

Gunathilake³

2021

J. Med. Plants Stud.

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Diabetes is one of the most challenging global health problems. Currently, reducing postprandial blood glucose concentration by inhibiting carbohydrate hydrolyzing enzymes is recognized as an effective treatment against diabetes. In this study, in vitro α-amylase and amyloglucosidase inhibitory effect of selected, underutilized and locally grown cereals (P. miliaceum, P. scrobiculatum and S. italica), yams (D. alata and D. esculenta) and root crops (L. spinosa and N. nucifera) were evaluated related to their dietary fiber contents. Results showed that the inhibitory effect of selected food crops has a positive correlation with their dietary fiber contents. L. spinosa with higher dietary fiber content generally had higher α-amylase (78.0%) and amyloglucosidase (71.28%) enzyme inhibitory percentages among yams and root crops. Significantly highest (p<0.05) dietary fiber content was in S. italic among three cereal varieties and it showed the highest inhibition activity against α-amylase (67.8%) and amyloglucosidase (59.40%) activity. These tested food crops are considerable sources of dietary fiber and are having αamylase and amyloglucosidase enzyme inhibitory actions against starch hydrolysis.

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“…In comparison, the fraction was rich in unsaturated lipids compared to their counterpart (saturated lipids) (Table 3). A literature check for the significance of these lipids suggest that 7,10hexadecadienoic acid, methyl ester had antioxidant, anti-inflammatory and antimicrobial activity, 33 hexadecanoic acid, ethyl ester has antioxidant and hypocholesterolemic activity 34 , cic-13-octadecenoic acid's bioactivity was unavailable, 35 while cis-vacenic acid has antioxidant activity. 36,37 It could be concluded that theses lipids may have contributed to the fraction's positive radical scavenging activity.…”

Section: Gcms Fatty Acid Profiling Of Fraction Of Hexane Leaf Extract...mentioning

confidence: 99%

In vitro Investigation and GC-MS Analysis of the Chemical Constituents in the Fraction of Hexane Leaf Extract of Tapinanthus bangwensis (Engl. and K. Krause) Loranthaceae

2024

TJPPS

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The exploration of medicinal plants as potential alternative therapeutic model comes from the quality content of its chemical compounds that exacerbate remarkable activity, nevertheless, there is the need for further exploit. This study investigated the in vitro antioxidant and antidiabetic potential of the fraction (HEX-ETACF or CF 2) of hexane leaf extract of Tapinanthus bangwensis, but importantly analyzed the likely chemical compounds present in the sample. The study used GC-MS (gas chromatography-mass spectroscopy) machine and spectrophotometer to elucidate the possible compounds and estimate the antioxidant and antidiabetic property respectively. The fraction had significant amount of alkaloids content, while phenolics content appeared higher than the flavonoids content The antioxidant results had the activity of lipid peroxy, 2,2-diphenyl-1-hydrazyl and nitrite radicals significantly inhibited compared to the ferric radicals. Furthermore, the result showed that the fraction inhibited α-amylase's metabolic activity higher than α-glucosidase. The Fourier transition infrared (FTIR) chromatogram had ten peaks, indicated the fraction contains complex molecules that bears carbonyl, methyl, carboxyl and aromatic groups respectively. The GC-MS chromatogram revealed twenty-one peaks, such that butyl-9-octadecenoic acid (8.01%) and nonahexacontanoic acid (0.05%) had the highest and lowest percentage areas (% area). Another GC-MS chromatogram had twenty-seven peaks, with squalene (peak 23; 37.29%) and 1,2-benzenedicarboxylic acid, buty-2-ethylhexyl ester (peak 27; 19.87%) been significantly abundant, suggested the fraction was rich in phenolics and phthalates. Our findings revealed that the compounds demonstrate beneficial effects as antioxidant and antidiabetic agents.

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In vitro α-Amylase and α-Glucosidase inhibitory activity of extracts of Feronia elephantum fruit and Paspalum scrobiculatum Grains.

Cited by 6 publications

References 13 publications

In vivo Anti-diabetic and Anti-hyperlipidemic Activities of ethyl Acetate/Methanol Fractions of Feronia elephantum Fruit in type 2 Diabetic Rats: Via α-amylase and PPAR-γ by using in silico Approach

In vivo Anti-diabetic and Anti-hyperlipidemic Activities of ethyl Acetate/Methanol Fractions of Feronia elephantum Fruit in type 2 Diabetic Rats: Via α-amylase and PPAR-γ by using in silico Approach

in vitro α amylase and amyloglucosidase inhibitory activities of selected underutilized cereals, yams and root crops

In vitro Investigation and GC-MS Analysis of the Chemical Constituents in the Fraction of Hexane Leaf Extract of Tapinanthus bangwensis (Engl. and K. Krause) Loranthaceae

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