In vivo actions of insulin-like growth factor-I (IGF-I) on bone formation and resorption in rats

Spencer, E. Martin; Liu, C.C.; Si, Efimenko; Howard, Guy A.

doi:10.1016/8756-3282(91)90050-s

Cited by 214 publications

(82 citation statements)

References 21 publications

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“…IGF-1 is present in bone tissue and induces osteoblast proliferation. Furthermore, IGF-1 induces stem cell migration via the PI-3-kinase (PI3K) signaling pathway (51,52). VEGF is known as the main regulator of angiogenesis and it cooperates in the osteogenesis process (53 previous studies are consistent with results of this study and support the hypothesis that MSC-CM promotes bone reconstruction.…”

Section: Discussionsupporting

confidence: 81%

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Sanchooli

Norouzian

Ardeshirylajimi

et al. 2017

Iran Red Crescent Med J

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Background: Tissue engineering is an available treatment for large bone defects. The therapeutic effects of mesenchymal stem cell (MSC) are mostly attributed to secretion of many cytokines and growth factors. Many factors of MSC secretions accumulate in a conditioned medium and these factors recruit native cells into a defect site to generate new bone tissues. Objectives: The aim of this study was to evaluate the influence of adipose tissue derived MSCs-conditioned medium (ADMSC-CM) on bone repair of rats with critical -size calvarial defect. Methods: This experimental study was performed at Shahid Beheshti University of Medical Sciences, Tehran, Iran, from 2016 to 2017. Conditioned medium was collected from healthy rat adipose tissue derived MSC (ADMSC) at passage four. Calvarial bone defect was created in hypothyroid rats using a dental bur. Sampling was taken by the linear-mono-gram method to determine sample size (n = 6 per group). The rats were divided randomly into four groups based on graft material as follows: empty defect, scaffold (Bio-Oss / type I collagen gel), scaffold / ADMSCs, scaffold /ADMSC-CM. Evaluations were made at 4 and 8 weeks after surgery using stereological analysis. Results: Histological analysis at 8 weeks indicated that the newly regenerated tissue almost covered the defect in the ADMSC-CM group. Stereological analysis showed that ADMSC-CM increased regenerated bone and numbers of osteocytes and osteoblasts compared with the defect and scaffold groups (P < 0.05). Also, bone regeneration was more effective in animals treated with ADMSC-CM than in those received ADMSC. Conclusions: These results suggest an important role for ADMSC-CM in bone regeneration, through trophic impact of its cytokines and growth factors that induce native cell proliferation and migration into the defect. Thus, ADMSC-CM seems to have good potential for application in bone tissue regeneration, in the cases of hypothyroidism.

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Section: Discussionsupporting

confidence: 81%

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Sanchooli

Norouzian

Ardeshirylajimi

et al. 2017

Iran Red Crescent Med J

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“…Aging is associated with decreases in growth hormone secretion and lowered serum levels of IGF-I [39], which are endocrine factors known to be anabolic for bone tissue. In fact, cultured osteoblasts show high affinity receptors and proliferative responses to each [40,41] and it is tempting to speculate that the decreased activity of the growth hormone-IGF-I axis (geriatric hyposomatotropism) might account, at least partially, for the age-related increase in bone fragility. The results of the present study support this concept and suggest that the longterm presence of IGF-I is important for skeletal integrity.…”

Section: Discussionmentioning

confidence: 99%

Factors Associated with Cortical and Trabecular Bone Loss as Quantified by Peripheral Computed Tomography (pQCT) at the Ultradistal Radius in Aging Women

et al. 1997

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Abstract. Peripheral quantitative computed tomography (pQCT) allows the separate determination of cortical and trabecular bone mineral density in the peripheral skeleton. This cross-sectional study was designed to examine the effects of healthy aging on pQCT measurements at the ultradistal radius. In a well-defined sample of 129 community-based women, aged 70-87 years, the differences in cortical and trabecular density over the age range were equivalent to losses of −0.41% and −0.65% per year, respectively. To investigate the mechanism of this agerelated decline, we assessed relationships between both parameters and height, weight, body mass index, dietary calcium intake, grip strength, and serum concentrations of insulin-like growth factor-I (IGF-I), calcidiol (25(OH)D 3 ), calcitriol (1,25(OH) 2 D 3 ), parathyroid hormone (PTH), and sex hormone binding globulin (SHBG). Multiple regression was used to adjust for potential confounders. Age was not significant after controlling for other covariables. Body mass index, grip strength, serum IGF-I, 25(OH)D 3 , and PTH (1-84) were found to be independent predictors of total bone density. Including (total or free) 1,25(OH) 2 D 3 did not improve the model precision. These findings provide evidence that, among other factors, the activity of the growth hormone-IGF-I-axis is of importance for skeletal integrity. Grip strength, serum IGF-I, and PTH (1-84) were discovered to be significantly related to cortical but not to trabecular density, suggesting that different mechanisms may be involved in compact and cancellous bone loss.Key words: Peripheral quantitative computed tomography (pQCT) -Age-related bone loss -Growth hormone -IGF-I.Low bone mass, as estimated by decreased bone mineral density (BMD), is an established predictor of osteoporotic fractures. Although fracture prediction by bone density is partially site-dependent [1], numerous reports have shown that fractures at all skeletal sites are significantly related to low bone mass at the distal forearm [2][3][4][5]. In all of these studies, BMD was assessed with single-photon absorptiometry. Peripheral quantitative computed tomography (pQCT) has been proposed as an adjunct to conventional densitometry [6,7]. BMD determination with pQCT at the site of the distal radius is a method of high precision and low radiation [8]. In addition, pQCT is the only noninvasive assessment of bone mineral to measure a true-dimensional density (as opposed to an areal density by standard methods) and to allow separate determination of cortical and trabecular bone density [9].Studies have indicated that bone loss in elderly women shows no evidence for an age-related leveling off [10][11][12][13]. Even at advanced ages, interventions to preserve or to enhance bone mass may therefore be an effective approach to prevention of fractures. However, the mechanisms leading to age-related bone loss remain poorly defined. Moreover, whether cortical and trabecular bone losses are mediated by differential mechanisms is virtually unknown. In the pres...

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“…In our study, BMD in LS was positively correlated with serum IGF-I levels, and patients with osteoporosis had serum IGF-I concentrations lower than patients without osteoporosis. On the basis of these findings and the known effects of IGF-I on skeletal metabolism, 33,34 we suggest that circulating levels of HEPATOLOGY Vol. 28, No.…”

Section: Discussionmentioning

confidence: 99%

Bone mineral density, serum insulin-like growth factor I, and bone turnover markers in viral cirrhosis

et al. 1998

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Previous studies suggest that low bone mass is a complication of alcoholic liver disease. Nevertheless, little is known about bone mass and bone metabolism in viral cirrhosis. To evaluate the prevalence and magnitude of hepatic osteopenia in these patients, bone remodeling status, and its relationship with the severity of liver disease and serum levels of insulin-like growth factor I (IGF-I), we studied 32 consecutive patients with viral cirrhosis and no history of alcohol intake. Bone mineral density (BMD) was measured by dual x-ray absorptiometry in the lumbar spine (LS) and femoral neck (FN), and the values were expressed as the z score. Bone metabolism markers and hormone profiles were measured. Patients with viral cirrhosis showed reduced BMD in all sites (LS: ؊1.27 ؎ 1.06, P F .001; FN: ؊0.48 ؎ 0.96; P F .01). Of the 32 patients, 53% met the diagnostic criteria for osteoporosis. In patients, urine deoxypyridinoline (D-Pyr) as a marker of bone resorption and serum bone alkaline phosphatase (b-AP) as a marker of bone formation were significantly higher than in control subjects (P F .001 and P F .01, respectively). Serum IGF-I was lower than in control subjects (P F .001), and significant differences were also found between patients with and without osteoporosis (P F .05). BMD in LS correlated with severity of the disease, with serum levels of IGF-I, and with urine D-Pyr. Our findings show that viral cirrhosis is a major cause of osteoporosis in men, and that low serum IGF-I levels seem to play a role in the bone mass loss in these patients. The biochemical markers of bone remodeling suggest high-turnover osteoporosis in patients with viral cirrhosis. (HEPATOLOGY 1998;28:695-699.)

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In vivo actions of insulin-like growth factor-I (IGF-I) on bone formation and resorption in rats

Cited by 214 publications

References 21 publications

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Factors Associated with Cortical and Trabecular Bone Loss as Quantified by Peripheral Computed Tomography (pQCT) at the Ultradistal Radius in Aging Women

Bone mineral density, serum insulin-like growth factor I, and bone turnover markers in viral cirrhosis

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