2020
DOI: 10.3389/fmicb.2020.582107
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In vivo Activity of Silver Nanoparticles Against Pseudomonas aeruginosa Infection in Galleria mellonella

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Cited by 18 publications
(11 citation statements)
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“…Both compounds were developed as topical antimicrobials to treat gonococcal infections and showed no efficacy systemically in preventing gonococcal-induced larval death. Interestingly, a recent study has reported that a different silver nanoparticle preparation that did not carry a bioactive ligand, such as MNBA, could increase the survival of larvae infected with P. aeruginosa [72]. The larval model therefore seems to be useful for comparing nanoparticle compounds, and disparities in bioactivity may reflect different mechanisms of action in vivo.…”
Section: Discussionmentioning
confidence: 99%
“…Both compounds were developed as topical antimicrobials to treat gonococcal infections and showed no efficacy systemically in preventing gonococcal-induced larval death. Interestingly, a recent study has reported that a different silver nanoparticle preparation that did not carry a bioactive ligand, such as MNBA, could increase the survival of larvae infected with P. aeruginosa [72]. The larval model therefore seems to be useful for comparing nanoparticle compounds, and disparities in bioactivity may reflect different mechanisms of action in vivo.…”
Section: Discussionmentioning
confidence: 99%
“…Prophylactic treatment (25 mg/kg) resulted in 80% larval survival for up to four days while larvae infected with a highly virulent clinical isolate of P. aeruginosa developed disease symptoms and/or died in the first 24 h. Prophylactic treatment enhanced haemocyte production and maintained phenoloxidase activity similar to uninfected larvae. Lower levels of phenoloxidase and the presence of haemocyte nodules (which entrap and kill invading microbes) in response to AgNPs explained the reduced levels of melanisation of treated larvae [ 18 , 19 ].…”
Section: G Mellonella Larvae As a Vehicle For In Vivo Antibacterial Drug Assessmentmentioning
confidence: 99%
“…Moreover, larvae receiving ZnO-NP pretreatment and C. albicans infection (1 × 10 5 cells per larva) were used for phenoloxidase activity detection at 3, 6, and 9 h after infection. The phenoloxidase activity assay was conducted according to a previous report (Thomaz et al, 2020). Briefly, 2 µl of hemolymph sample was diluted in 18 µl of Tris buffer saline containing 5 mM CaCl 2 , followed by dilution with 180 µl of 2 mM L-DOPA (Solarbio, Beijing, China) in 50 mM sodium phosphate at pH 6.5.…”
Section: Phenoloxidase Activity Assaymentioning
confidence: 99%