In vivo analgesic activities and safety assessment of Vitis vinifera L and Punica granatum L fruits extracts

Zeghad, Nadia; Aicha, None Madi; Sihem, Helmi; Belkhiri, Abdelmalik

doi:10.4314/tjpr.v16i3.8

Cited by 6 publications

(3 citation statements)

References 4 publications

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“…Numerous studies have shown that horse chestnut seeds contain several escins which dose-dependently inhibited the increase of vascular permeability and showed good anti-inflammatory effects [19,41,42]. The grapevine extracts are rich in polyphenols such as flavonoids, catechins, and stilbenes [43]. Among them, caffeic acid, quercetin, kaempferol glycosides, and resveratrol were identified as the major compounds of extracts with anti-inflammatory and analgesic activity [29,44].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

et al. 2019

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The aim of this work was to assess the analgesic effect of three Vitis vinifera L. leaf extracts and the anti-inflammatory effect of three gels obtained from Aesculus hippocastanum L. seed extracts using animal models, as a preliminary study for the future development of topical preparations based on the combination of extracts with synergistic therapeutic effects on hemorrhoid disease. The analgesic effect was determined by means of the writhing test in mice. The anti-inflammatory effect was determined after administration of carrageenan or kaolin in the rat paw. Extraction using glycerol yielded the highest amounts of flavonoids for both V. vinifera leaves (37.27 ± 1.174 mg/L) and A. hippocastanum seeds (53.48 ± 0.212 mg/L). The highest total phenolic contents were registered for the V. vinifera 20% ethanolic extract (615.3 ± 34.44 mg/L) and for the A. hippocastanum glycerolic extract (247.8 ± 6.991 mg/L). The writhing test revealed that the V. vinifera ethanolic extract induced the most efficient analgesia (57.20%, p < 0.01), better than that induced by the positive control. In the carrageenan inflammation model, only the gel obtained from the A. hippocastanum glycerolic extract significantly reduced paw edema (17.27%, p < 0.05). An anti-inflammatory effect was also observed in the kaolin inflammation model but was not statistically significant (10.12%, p > 0.05). Our findings indicate that V. vinifera and A. hippocastanum extracts may have potential uses for the treatment of pain and inflammation associated with hemorrhoid disease.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

et al. 2019

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“…The hot plate test was used to measure response latency time according to the method described by Zeghad [33,21]. Five groups of rats (6 rats per group) were used.…”

Section: Central Analgesic Assay a Hot Plate Testmentioning

confidence: 99%

Phytochemical Investigation of Algerian Ceratonia siliqua L. Leaves Extract, by Evaluation of Antioxidants, and Analgesic Effects

Madi

MAAMERI²,

SIHEM³

et al. 2022

Egypt. J. Chem.

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The present study reports on the investigation of phenolic compounds and the determination of the biological activities, toxicity and analgesic effect Ceratonia siliqua, phenolic content was spectrophotometrically quantified with the microplate method, while the bioactivities of methanolic and aqueous extracts were assessed by determining of antioxidant activity. The methanolic extract showed high contents of total flavonoids and exhibited the highest in vitro antioxidant potential (DPPH, ABTS, Superoxide, FRAP, CUPRAC, and phenanthrolin). In the acute toxicity, no death was reported in a dose of 1000, and 1500mg /Kg BW, however the dose of 2000mg/kg BW showed many signs of toxicit y, so the DL50 is higher than 2000mg/Kg BW. In analgesic in vivo assay, the methanolic extract showed a very important capacity to reduce pain, whether central or peripheral, with a certain dose-dependent relationship. For the three tests (hot plate, tail flick and acetic acid assay), the effective dose was 500mg/kg and the effective duration was 30min to 1 hour. This study demonstrate interesting in vitro and in vivo effects of carob leaves extracts and suggests that could be used as an alternative to chemical compounds. According to the extracts concentration and nature, it is very interesting to use like a source of natural antioxidants and analgesic compounds that might be more widely used in pharmaceutical industries.

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“…Pomegranate possesses a broad array of pharmacological properties such as antioxidant (3), anti-inflammatory (4), anti-cancer (5,6), anti-parasitic (7), analgesic (8), antimicrobial (9), neuroprotective (10), antifungal (11), antiulcer (12), and antidiarrheal (13). The flowers of pomegranate have been ethnomedically used for their anti-cholinesterase, anti-hyperglycemic (14), anti-diabetic, anti-obesity (15), antibacterial, and antioxidant (16) effects.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of phytochemical contents and antioxidant activity of pomegranate flower

Küçükbay

Tekin

2020

Journal of the Turkish Chemical Society Section A: Chemistry

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Pomegranate is one of the most abundant fruits consumed in Turkey. This study aimed to determine the content of total phenolic, total flavonoid, and antioxidant activity of Punica granatum L. flower in different extracts. Antioxidant activities of different extracts were determined 2,2-diphenyl-1picrylhydrazyl (DPPH•) radical scavenging, reducing power, and metal chelating methods. The results showed that the total phenolic content for the extracts ranged from 14.82 to 90.86 mg gallic acid equivalents (GAE) / g extract. The contents of flavonoids were found to range from 7.35 to 500.00 mg quercetin equivalents (QUE)/ g extract. All pomegranate flower extracts displayed remarkable antioxidant activity according to DPPH and reducing power assays. Especially the methanolic extract of pomegranate flower possesses significant scavenging activity against DPPH• (85.80 %), as well as the largest contents of flavonoids and phenolic compounds. The antioxidant capacity of the methanolic extract was also greater than those of BHT and α-tocopherol in DPPH and reducing power assays. The results demonstrated that the antioxidant activity of extracts of Punica granatum L. flower might, at least in part, have high content of flavonoids and other phenolics.

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In vivo analgesic activities and safety assessment of Vitis vinifera L and Punica granatum L fruits extracts

Cited by 6 publications

References 4 publications

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

Phytochemical Investigation of Algerian Ceratonia siliqua L. Leaves Extract, by Evaluation of Antioxidants, and Analgesic Effects

Evaluation of phytochemical contents and antioxidant activity of pomegranate flower

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