In Vivo Analysis with Electrochemical Sensors and Biosensors

Xiao, Tongfang; Wu, Fei; Hao, Jie; Zhang, Meining; Yu, Ping

doi:10.1021/acs.analchem.6b04308

Cited by 176 publications

(114 citation statements)

References 207 publications

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“…The analysis of the properties of biological organisms and their cellular and sub‐cellular components with high spatial and temporal resolution has resulted in the development of a range of optical, electronic, and analytic biosensors . Once cells are broken apart or lysed, identification and analysis of biomolecules can be efficiently carried out with existing biosensors, but more recently there is a push to enable sensing of biomolecular and/or electrical activity of entire organelles, cells, or organs in‐situ . One of the central challenges in this endeavor is that organelles, cells, and organs are 3D, while many chip‐based biosensing platforms are 2D .…”

Section: Origami Biosensorsmentioning

confidence: 99%

Origami Biosystems: 3D Assembly Methods for Biomedical Applications

et al. 2018

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Conventional assembly of biosystems has relied on bottom‐up techniques, such as directed aggregation, or top‐down techniques, such as layer‐by‐layer integration, using advanced lithographic and additive manufacturing processes. However, these methods often fail to mimic the complex three dimensional (3D) microstructure of naturally occurring biomachinery, cells, and organisms regarding assembly throughput, precision, material heterogeneity, and resolution. Pop‐up, buckling, and self‐folding methods, reminiscent of paper origami, allow the high‐throughput assembly of static or reconfigurable biosystems of relevance to biosensors, biomicrofluidics, cell and tissue engineering, drug delivery, and minimally invasive surgery. The universal principle in these assembly methods is the engineering of intrinsic or extrinsic forces to cause local or global shape changes via bending, curving, or folding resulting in the final 3D structure. The forces can result from stresses that are engineered either during or applied externally after synthesis or fabrication. The methods facilitate the high‐throughput assembly of biosystems in simultaneously micro or nanopatterned and layered geometries that can be challenging if not impossible to assemble by alternate methods. The authors classify methods based on length scale and biologically relevant applications; examples of significant advances and future challenges are highlighted.

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Section: Origami Biosensorsmentioning

confidence: 99%

Origami Biosystems: 3D Assembly Methods for Biomedical Applications

et al. 2018

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“…[6] The reliable and accurate detection of DA, however, is not easy. [7] The widely adopted in vitro methods by medical researchers, including the conventional chromatography or capillary electrophoresis, suffer from sophisticated pre-treatment processes and a long operation time. [8,9] The alternative colorimetric or fluorescent optical detection strategies exhibit simplified procedures as well as enhanced sensitivity, but have so far not been applied very often to the intact biological samples or living cells.…”

Section: Highly Sensitive and Selective Electrochemical Detection Of mentioning

confidence: 99%

Highly Sensitive and Selective Electrochemical Detection of Dopamine using Hybrid Bilayer Membranes

et al. 2018

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The rapid and precise measurement of dopamine (DA) levels is of great benefit to unveil physiological and pathological processes. The electrochemical detection approaches feature fast DA response, but remain challenging at the moment to realize ultra-high sensitivity and selectivity to the trace amount of DA, especially in the presence of highly concentrated ascorbic acid (AA, the most common interferent in the human body). To address this issue, a negatively charged hybrid bilayer membrane (HBM) modified gold electrode was designed, which was only attractive to the positive DA but not the negative AA. As a result, this work managed to achieve an ultra-low detection limit of 0.41 pM DA under the interference of AA of 10 9 times higher concentration. Such an excellent performance is over three orders of magnitude better than previously reported electrochemical sensing methods, suggesting the promise of applying HBM functionalization in electrochemical and bio-sensing applications.

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“…It is the essential groundwork to fabricate enzymatic electrodes for constructing enzyme-based electrochemical biosensors and enzymatic biofuel cells (BFCs) [1] [2] [3] [4] [5]. Glucose-responsive enzymatic electrodes, which are responsible for electrochemical glucose biosensors or bioanodes of glucose-based BFCs with glucose as fuels, have attracted much attention [6] [7] [8] [9] [10]. Two types of commercially available enzymes, glucose oxidase (GOx) and glucose dehydrogenases (GDH), are generally employed to construct glucose-responsive enzymatic electrodes [1]- [10].…”

Section: Introductionmentioning

confidence: 99%

“…Glucose-responsive enzymatic electrodes, which are responsible for electrochemical glucose biosensors or bioanodes of glucose-based BFCs with glucose as fuels, have attracted much attention [6] [7] [8] [9] [10]. Two types of commercially available enzymes, glucose oxidase (GOx) and glucose dehydrogenases (GDH), are generally employed to construct glucose-responsive enzymatic electrodes [1]- [10]. The GDH family is classified into three different types on the basis of its cofactors, nicotinamide adenine dinucleotide-dependent GDH (NAD-GDH), flavin adenine dinucleotide-dependent GDH (FAD-GDH), and pyrroloquinoline quinone-dependent GDH (PQQ-GDH) [11]- [16].…”

Section: Introductionmentioning

confidence: 99%

A Glucose-Responsive Enzymatic Electrode on Carbon Nanodots for Glucose Biosensor and Glucose/Air Biofuel Cell

Gao¹,

Wu²,

Gao³

2019

AJAC

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In this study, an enzymatic electrode for glucose biosensing and bioanode of glucose/air biofuel cell has been fabricated by immobilizing poly (methylene green) (polyMG) for electrocatalytic NADH oxidation and NAD + -dependent glucose dehydrogenase (GDH) for oxidizing glucose on carbon nanodots (CNDs). The polyMG-CNDscomposites obtained by electro-polymerization of dye MG molecules adsorbed on CNDs display excellent electrocatalytic activity toward NADH electro-oxidation at a low overpotential of ca. −0.10 V (vs. Ag/AgCl) and the integrated enzymatic electrode shows fast response to glucose electrooxidation. Using the fabricated GDH-based enzymatic electrode, a glucose biosensor was constructed and exhibits a wide linear dynamic range from 0 to 8 mM, a low detection limit of 0.02 μM (S/N = 3), and fast response time (ca. 4 s) under the optimized conditions. The developed glucose biosensor was used to detect glucose content in human blood with satisfactory results. The fabricated GDH-based enzymatic electrode was also employed as bioanode to assembly a glucose/air biofuel cell with the laccase-CNDs/GC as the biocathode. The maximum power density delivered by the assembled glucose/air biofuel cell reaches 3.1 μW•cm −2 at a cell voltage of 0.22 V in real sample fruit juice. The present study demonstrates that potential applications of GDH-based CNDs electrode in analytical and biomedical measurements.

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In Vivo Analysis with Electrochemical Sensors and Biosensors

Cited by 176 publications

References 207 publications

Origami Biosystems: 3D Assembly Methods for Biomedical Applications

Origami Biosystems: 3D Assembly Methods for Biomedical Applications

Highly Sensitive and Selective Electrochemical Detection of Dopamine using Hybrid Bilayer Membranes

A Glucose-Responsive Enzymatic Electrode on Carbon Nanodots for Glucose Biosensor and Glucose/Air Biofuel Cell

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