In-Vivo and Fast Examination of Iron Concentration of Magnetic Nano-Particles in an Animal Torso via Scanning SQUID Biosusceptometry

Tseng, W. K.; Chieh, Jen Jie; Yang, Shieh Yueh; Horng, Herng Er; Hong, Chin Yih; Yang, Hong-Chang; Wu, Ching-Chou

doi:10.1109/tasc.2011.2107871

Cited by 6 publications

(16 citation statements)

References 15 publications

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“…Furthermore, I max decreased as the reagent concentration was reduced from 0.3 to 0.005 emu/g, with Fe concentration ranging from 1.95 mg/g to 32.5 μg/g. This shows that the detectable distance was approximately 56 mm for the samples with a concentration of 0.1–0.3 emu/g, whereas that for the samples with a concentration of 0.005–0.01 emu/g was approximately 44 mm, which is sufficient for detecting MNPs in tumors implanted in the livers or grafted onto the backs of animals [ 22 , 23 ]. In other words, the sensitivity to the amount of Fe in the MNPs was approximately 250 μg at 56 mm and 12.5 μg at 44 mm.…”

Section: Resultsmentioning

confidence: 99%

Dual-imaging model of SQUID biosusceptometry for locating tumors targeted using magnetic nanoparticles

Chieh

Huang

Lee

et al. 2015

Journal of Nanobiotechnology

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BackgroundFor intraoperative imaging in operating theaters or preoperative imaging in clinics, compact and economic integration rather than large and expensive equipment is required to coregister structural and functional imaging. However, current technologies, such as those integrating optical and gamma cameras or infrared and fluorescence imaging, involve certain drawbacks, including the radioactive biorisks of nuclear medicine indicators and the inconvenience of conducting measurements in dark environments.MethodsTo specifically and magnetically label liver tumors, an anti-alpha-fetoprotein (AFP) reagent was synthesized from biosafe iron oxide magnetic nanoparticles (MNPs) coated with anti-AFP antibody and solved in a phosphate buffered saline solution. In addition, a novel dual-imaging model system integrating an optical camera and magnetic scanning superconducting-quantum-interference device (SQUID) biosusceptometry (SSB) was proposed. The simultaneous coregistration of low-field magnetic images of MNP distributions and optical images of anatomical regions enabled the tumor distribution to be determined easily and in real time. To simulate targeted MNPs within animals, fewer reagents than the injected dose were contained in a microtube as a sample for the phantom test. The phantom test was conducted to examine the system characteristics and the analysis method of dual images. Furthermore, the animal tests were classified into two types, with liver tumors implanted either on the backs or livers of rats. The tumors on the backs were to visually confirm the imaging results of the phantom test, and the tumors on the livers were to simulate real cases in hepatocellular carcinoma people.ResultsA phantom test was conducted using the proposed analysis method; favorable contour agreement was shown between the MNP distribution in optical and magnetic images. Consequently, the positioning and discrimination of liver tumors implanted on the backs and livers of rats were verified by conducting in vivo and ex vivo tests. The results of tissue staining verified the feasibility of using this method to determine the distribution of liver tumors.ConclusionThe results of this study indicate the clinical potential of using anti-AFP-mediated MNPs and the dual-imaging model SSB for discriminating and locating tumors.

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Section: Resultsmentioning

confidence: 99%

Dual-imaging model of SQUID biosusceptometry for locating tumors targeted using magnetic nanoparticles

Chieh

Huang

Lee

et al. 2015

Journal of Nanobiotechnology

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“…This intermittently dynamic in vivo magnetic examination differed from the continuously static and in vivo measurements that we performed in preliminary studies [19]–[20]. However, the expression of both sets of in vivo data using ΔM were normalized to the maximum level (ΔM/M max ) for comparison, because the MNP distribution in the rat body is too complex to simulate the known magnetism of different individuals (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

A Noninvasive Method to Determine the Fate of Fe3O4 Nanoparticles following Intravenous Injection Using Scanning SQUID Biosusceptometry

et al. 2012

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Magnetic nanoparticles (MNPs) of Fe3O4 have been widely applied in many medical fields, but few studies have clearly shown the outcome of particles following intravenous injection. We performed a magnetic examination using scanning SQUID biosusceptometry (SSB). Based on the results of SSB analysis and those of established in vitro nonmagnetic bioassays, this study proposes a model of MNP metabolism consisting of an acute metabolic phase with an 8 h duration that is followed by a chronic metabolic phase that continues for 28 d following MNP injection. The major features included the delivery of the MNPs to the heart and other organs, the biodegradation of the MNPs in organs rich with macrophages, the excretion of iron metabolites in the urine, and the recovery of the iron load from the liver and the spleen. Increases in serum iron levels following MNP injection were accompanied by increases in the level of transferrin in the serum and the number of circulating red blood cells. Correlations between the in vivo and in vitro test results indicate the feasibility of using SSB examination for the measurement of MNP concentrations, implying future clinical applications of SSB for monitoring the hematological effects of MNP injection.

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“…To examine the magnetic characteristics of magnetic labeling, the in-vivo tests were processed using developed scanning SQUID biosusceptometry (SSB) [13][14][15] based on AC susceptibility of samples and MRI based on the distortion of the imaging field that resulted from MNPs. 11 Following standard procedures, the test mice in these in-vivo tests were anesthetized using inhalation anesthesia.…”

Section: In-vivo Testsmentioning

confidence: 99%

“…This characteristic has recently been proven for free MNPs in animals using scanning superconducting-quantum-interferencedevice (SQUID) biosusceptometry (SSB). [13][14][15] The feasibility for detecting bound MNPs on tumors has yet to be tested. If such a procedure is effective, SSB could be used as an intraoperative instrument because SSB possesses the advantages of high sensitivity of a SQUID sensor, high examination mobility around the torso, easy and practical operation, and high safety and more cost-effectiveness of low AC-field excitation.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

Huang¹,

Chieh²,

Horng³

et al. 2012

IJN

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For preoperative and intraoperative detection of tumor distribution, numerous multimodal contrast agents, such as magnetic nanoparticles (MNPs) with several examination indicators, are currently in development. However, complex materials, configuration, and cost are required for multimodal contrast agents, accompanied by a high possibility of toxicity and low popularity in clinics. Nevertheless, the magnetic labeling of MNPs using bioprobes should be feasible not only in preoperative magnetic resonance imaging (MRI), but also in intraoperative examination based on other magnetic properties. In this study, anti-alpha-fetoprotein (AFP)-mediated Fe 3 O 4 MNPs, injected into mice with liver tumors, were used to examine the characteristics of magnetic labeling. Using MRI and scanning superconducting-quantum-interference-device biosusceptometry (SSB), based on alternating current (AC) susceptibility, the magnetic labeling occurred significantly on the first day post-injection of anti-AFP magnetic fluid (MF), and then decreased over time. However, for both MF without antibodies and an anti-carcinoembryonic antigen MF, no magnetic labeling occured on the first day of their respective post-injection. The favorable agreement indicates that magnetic labeling possesses two magnetic characteristics: distortion of the imaging field and AC susceptibility. In addition, the results of the biopsy tests, anti-AFP staining, and Prussian blue staining show the same dynamics as those of magnetic methodologies and prove that bound MNPs on tumor tissue are rotatable by an AC magnetic field to express AC susceptibility. Therefore, with the simple configuration of antibody-mediated MNPs, magnetic labeling is also feasible for intraoperative examinations using SSB with high mobility and sensitivity.

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In-Vivo and Fast Examination of Iron Concentration of Magnetic Nano-Particles in an Animal Torso via Scanning SQUID Biosusceptometry

Cited by 6 publications

References 15 publications

Dual-imaging model of SQUID biosusceptometry for locating tumors targeted using magnetic nanoparticles

Dual-imaging model of SQUID biosusceptometry for locating tumors targeted using magnetic nanoparticles

A Noninvasive Method to Determine the Fate of Fe3O4 Nanoparticles following Intravenous Injection Using Scanning SQUID Biosusceptometry

Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

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