In vivo and in silico analyses of estrogenic potential of bisphenol analogs in medaka (Oryzias latipes) and common carp (Cyprinus carpio)

Yamaguchi, Akemi; Ishibashi, Hiroshi; Arizono, Koji; Tominaga, Nobuaki

doi:10.1016/j.ecoenv.2015.06.014

Cited by 37 publications

(25 citation statements)

References 37 publications

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“…The in vivo estrogenic activity of bisphenols have been studied in fish and rodents. In adult male medaka, BPAF and BPC are more potent than BPA in inducing hepatic upregulation of the expression of vtg and other estrogenic biomarkers (Yamaguchi et al, 2015). Moreover, the bisphenols induce uterine weight gain in ovariectomized rats, and the uterotrophic effect is more pronounced for BPC and BPAF compared to BPA (Conley et al, 2016).…”

Section: Discussionmentioning

confidence: 97%

Differential activity of BPA, BPAF and BPC on zebrafish estrogen receptors in vitro and in vivo

Pinto

Hao

Grimaldi

et al. 2019

Toxicology and Applied Pharmacology

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The high volume production compound bisphenol A (BPA) is of environmental concern largely because of its estrogenic activity. Consequently, BPA analogues have been synthesized to be considered as replacement molecules for BPA. These analogues need to be thoroughly evaluated for their estrogenic activity. Here, we combined mechanism zebrafish-based assays to examine estrogenic and anti-estrogenic activities of BPA and two of its analogues, bisphenol AF (BPAF) and bisphenol C (BPC) in vitro and in vivo. In vitro reporter cell lines were used to investigate agonistic and antagonistic effects of the three bisphenols on the three zebrafish estrogen receptors. The transgenic Tg(5×ERE:GFP) and Cyp19a1b-GFP zebrafish lines were then used to analyze estrogenic and anti-estrogenic responses of the three bisphenols in vivo. BPA, BPAF and BPC were agonists with different potencies for the three zebrafish estrogen receptors in vitro. The

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Section: Discussionmentioning

confidence: 97%

Differential activity of BPA, BPAF and BPC on zebrafish estrogen receptors in vitro and in vivo

Pinto

Hao

Grimaldi

et al. 2019

Toxicology and Applied Pharmacology

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“…Bisphenol A (BPA), is a well-known endocrine disruptor that affects skeletal development and bone maintenance through altered Er signaling (Yamaguchi et al, 2015;Tohyama et al, 2015). In addition, it is also a modulator of the activity of signaling pathways related to progesterone receptor, peroxisome proliferator-activated receptor, estrogen-related receptors, Ar, thyroid hormone receptors and Pxr (Funabashi et al, 2004;Naciff et al, 2005;Oshida et al, 2015).…”

Section: Estrogen Receptorsmentioning

confidence: 99%

Fish as a model to assess chemical toxicity in bone

et al. 2018

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Environmental toxicology has been expanding as growing concerns on the impact of produced and released chemical compounds over the environment and human health are being demonstrated. Among the toxic effects observed in organisms exposed to pollutants, those affecting skeletal tissues (osteotoxicity) have been somehow overlooked in comparison to hepato-, immune-, neuro- and/or reproductive toxicities. Nevertheless, sub-lethal effects of toxicants on skeletal development and/or bone maintenance may result in impaired growth, reduced survival rate, increased disease susceptibility and diminished welfare. Osteotoxicity may occur by acute or chronic exposure to different environmental insults. Because of biologically and technically advantagous features - easy to breed and inexpensive to maintain, external and rapid rate of development, translucent larvae and the availability of molecular and genetic tools - the zebrafish (Danio rerio) has emerged in the last decade as a vertebrate model system of choice to evaluate osteotoxicity. Different experimental approaches in fish species and analytical tools have been applied, from in vitro to in vivo systems, from specific to high throughput methodologies. Current knowledge on osteotoxicity and underlying mechanisms gained using fish, with a special emphasis on zebrafish systems, is reviewed here. Osteotoxicants have been classified into four categories according to the pathway involved in the transduction of the osteotoxic effects: activation/inhibition of membrane and/or nuclear receptors, alteration of redox condition, mimicking of bone constituents and unknown pathways. Knowledge on these pathways is also reported here as it may provide critical insights into the development, production and release of future chemical compounds with none or low osteotoxicity, thus promoting the green/environmental friendly chemistry.

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“…Transcription of these genes is regulated by the binding of the estrogen (ligand)‐ER homodimeric complex to the estrogen responsive elements (ERE) that are located in the promoter regions of Vtg and Chg . Various environmental estrogens such as antibacterial agent, alkylphenolic compounds and synthetic estrogens are known to induce Vtg and/or Chg protein synthesis in male medaka (Ishibashi et al, , ; Yamaguchi et al, , ) as in the same or similar mechanism to the natural estrogens.…”

Section: Introductionmentioning

confidence: 99%

Gene expression analyses of vitellogenin, choriogenin and estrogen receptor subtypes in the livers of male medaka (Oryzias latipes) exposed to equine estrogens

et al. 2016

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In the present study, we investigated transcriptional profiles of estrogen-responsive genes, such as vitellogenins (Vtg1 and Vtg2), choriogenins (ChgL and ChgH) and estrogen receptor subtypes (ERα, ERβ1, and ERβ2), in the liver of male medaka fish (Oryzias latipes) that were exposed to six equine estrogens (1-300 ng l(-1) ) for 3 days. Our quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses revealed that the expression levels of hepatic Vtg, Chg and ERα genes in male medaka responded to various types and concentrations of equine estrogens. The estrogenic potentials of the tested chemicals were in the order of equilin > 17β-estradiol > equilenin > 17β-dihydroequilin > 17β-dihydroequilenin > 17α-dihydroequilin > 17α-dihydroequilenin, showing the higher estrogenic potential of equilin than that of 17β-estradiol. Our results also showed that the estrogenicities of 17β-dihydroequilin and 17β-dihydroequilenin were more potent than that of 17α-dihydroequilin and 17α-dihydroequilenin. Furthermore, in gene expression analyses of hepatic ER subtypes, observations were made to note that 17β-estradiol and equilin induced ERα transcription in male medaka, and the ERα transcription level had significantly positive correlations with the expression of Vtg and Chg genes. In contrast, in the same 17β-estradiol and equilin treatment groups, it was shown that the transcription levels of hepatic ERβ1 and/or ERβ2 had significantly negative correlations with the expression of Vtg and Chg genes. These results suggested some potential involvement of the ER subtypes in the regulation of Vtg and Chg gene expressions in the liver. This is the first report describing the comprehensive analyses of in vivo estrogenicity of the equine estrogens in male medaka. Copyright © 2016 John Wiley & Sons, Ltd.

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In vivo and in silico analyses of estrogenic potential of bisphenol analogs in medaka (Oryzias latipes) and common carp (Cyprinus carpio)

Cited by 37 publications

References 37 publications

Differential activity of BPA, BPAF and BPC on zebrafish estrogen receptors in vitro and in vivo

Differential activity of BPA, BPAF and BPC on zebrafish estrogen receptors in vitro and in vivo

Fish as a model to assess chemical toxicity in bone

Gene expression analyses of vitellogenin, choriogenin and estrogen receptor subtypes in the livers of male medaka (Oryzias latipes) exposed to equine estrogens

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