In vivo and in vitro evaluation of erianin, a novel anti-angiogenic agent

Gong, Yanqing; Fan, Yuqi; Wu, Di; Yang, Hao; Zhi-bi, HU; Wang, Z.-T

doi:10.1016/j.ejca.2004.01.041

Cited by 114 publications

(57 citation statements)

References 30 publications

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“…A well-grown plant bears up to 20 well-spaced flowers on a spike, which are prised for their exceptional fragrance. In addition, several important secondary metabolites have recently been identified from D. chrysotoxum (Gong et al 2004;Yang et al 2004a, b). Notable among them is the bisbenzyl erianin, which has tremendous therapeutic potential as antioxidant, antiangiogenic and antitumour agent (Ng et al 2000;Gong et al 2004).…”

Section: Introductionmentioning

confidence: 97%

Direct and callus-mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae)

Roy

Naha

Majumdar

et al. 2007

Plant Cell Tiss Organ Cult

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An efficient method of mass propagation of Dendrobium chrysotoxum Lindl. was developed using a shoot-tip culture system. Both direct and callus-mediated formation of protocorm-like bodies (PLBs) occurred from the basal cut surface of explants. Frequency of callusing was best in the presence of 2 mM thidiazuron (TDZ) or N 6 -benzylaminopurine (BAP). The callus exhibited complete hormone autonomy for growth and differentiation of PLBs and was maintained for 18 months without any exogenous growth regulators, an aspect important for minimising somaclonal variation. However, the rate of callus growth and PLB formation varied with application of cytokinin and auxin. In addition, the callus exhibited a differential sensitivity to the exogenous cytokinins. While BAP promoted callus growth and PLB differentiation, TDZ was inhibitory to callus mediated PLB formation and caused extensive necrosis of callus. Although a-naphthaleneacetic acid (NAA) had no significant effect on the induction of callus, subsequent growth was best in its presence. Using a 3-month subculture period, a 69-fold increase in callus weight was achieved with 0.5 mM NAA, while as many as 133 PLBs could be obtained per 100 mg callus in the presence of 1 mM NAA. For direct PLB formation, the optimum cytokinin dosage was dependent upon the type of cytokinin used. While TDZ was most effective at a concentration of 1 mM (15 PLBs per explant), for similar PLB yield the application of 8 mM BAP was essential.

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Section: Introductionmentioning

confidence: 97%

Direct and callus-mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae)

Roy

Naha

Majumdar

et al. 2007

Plant Cell Tiss Organ Cult

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“…The products prepared from the dried stems of Dendrobium plants are also used as precious health foods and nutrients [2]. Earlier work on the genus led to the isolation of a series of compounds such as alkaloids, fluorenones, sesquiterpenoids, bibenzyls, and phenanthrenes with antioxidant, antitumor, antimutagenic, and other activities [3][4][5][6]. D. longicornu Lindl is distributed in Nepal, Sikkim, Bhutan, India, Vietnam, and the southwestern part of China [7].…”

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confidence: 99%

Mono-aromatic constituents of Dendrobium longicornu

Yin

Liu

et al. 2009

Chem Nat Compd

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The stems of several Dendrobium species (Orchidaceae) are used in traditional Chinese medicine as a tonic to nourish the stomach, promote the production of body fluid, and reduce fever [1]. The products prepared from the dried stems of Dendrobium plants are also used as precious health foods and nutrients [2]. Earlier work on the genus led to the isolation of a series of compounds such as alkaloids, fluorenones, sesquiterpenoids, bibenzyls, and phenanthrenes with antioxidant, antitumor, antimutagenic, and other activities [3][4][5][6]. D. longicornu Lindl is distributed in Nepal, Sikkim, Bhutan, India, Vietnam, and the southwestern part of China [7]. Previously, there have been no reports on its chemical constituents. In the course of our search for new bioactive natural products from medicinal plants in Yunnan of China, we investigated the plant.D. longicornu was collected from Lianghe County of Yunnan Province, China in September, 2004. The air-dried whole plants (3.4 kg) were chopped and extracted with 95% EtOH five times (each 20 L) at room temperature. The EtOH extract (115 g) was diluted with H 2 O (1 L) and then extracted with petroleum ether, EtOAc, and n-BuOH (each 0.5 L, five times) successively. Evaporation of the respective solvents gave the petroleum ether (30 g), EtOAc (26 g), and n-BuOH (52 g) extracts.The EtOAc extract (26 g) was applied to a silica gel column (200-300 mesh, 100 cm × 10 cm), eluting with petroleum ether containing increasing amounts of acetone to obtain six fractions monitored by TLC tests. Spots on the plate were observed under UV light and visualized by spraying with 15% H 2 SO 4 in ethanol followed by heating. Fraction 1 (2 g) was further purified on column chromatography (silica gel, 200-300 mesh, 60 cm × 4 cm, petroleum ether-acetone 30:1) to afford five subfractions. The second and third subfractions were isolated on preparative TLC (20 cm × 20 cm, petroleum ether-benzene 1:1) to afford 1 (40 mg) and 2 (35 mg), respectively. The fourth subfraction was purified by recrystallization from acetone to obtain 8 (100 mg); Fr. 2 (4.5 g) was separated on column chromatography (silica gel, 200-300 mesh, 60 cm × 5 cm, CHCl 3 -acetone 15:1) to yield five subfractions. The second subfraction was isolated on a Sephadex LH-20 column (120 cm × 3 cm, MeOH) to obtain 5 (15 mg). The third subfraction was purified on a preparative TLC plate (20 cm × 20 cm, petroleum etherEtOAc 9:1) to afford 7 (8 mg). The fourth subfraction was separated on a preparative TLC plate (20 cm × 20 cm, CHCl 3 -acetone 15:1) to afford 6 (6 mg); Fr. 5 (9 g) was purified on column chromatography (silica gel, 200-300 mesh, 60 cm × 4 cm, CHCl 3 -acetone 10:1) to afford two subfractions. The second subfraction (3 g) was subjected to repeated column chromatography similarly, first on Sephadex LH-20 (MeOH), and then on preparative TLC (20 cm × 20 cm, petroleum etherEtOAc 2:1, CHCl 3 -MeOH 15:1) to isolate 3 (11 mg) and 4 (20 mg). OR O OR O 1, 2 1: R = CH 2 CH(C 2 H 5 )(CH 2 ) 3 CH 3 2: R = (CH 2 ) 3 CH 3 CHO OH HO CH 3 COOC ...

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“…5,6 Some phenolics from Dendrobium species have been reported the antitumor activity ever by some researcher, 3,22,23 thus, compounds 1-2 were assessed for cytotoxic activity against two human tumor cell lines (A549 and HL-60). Compound 2 has a week effect for inhibition ratio of 61.9% at the concentration up to10 -5 mol/L and compound 1 has no effect.…”

Section: Resultsmentioning

confidence: 99%

Chemical Components of Dendrobium polyanthum

2009

Bulletin of the Korean Chemical Society

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A new tetrahydroanthracene, 3,6,9-trihydroxy-3,4-dihydroanthracen-1(2H)-one (1), six phenolics, moscatilin (2), gigantol (3), batatasin (4), moscatin (5), 9,10-dihydromoscatin (6), 10-dihydrophenanthrene-2,4,7-triol (7), and a sesquiterpenoid, corchoionoside C (8), together with two sterols β-sitosterol (9) and daucosterol (10), were isolated from the stems of Dendrobium polyanthum. Compounds 1 and 2 were assessed for cytotoxic activity against two human tumor cell lines (A549 and HL-60).

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In vivo and in vitro evaluation of erianin, a novel anti-angiogenic agent

Cited by 114 publications

References 30 publications

Direct and callus-mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae)

Direct and callus-mediated protocorm-like body induction from shoot-tips of Dendrobium chrysotoxum Lindl. (Orchidaceae)

Mono-aromatic constituents of Dendrobium longicornu

Chemical Components of Dendrobium polyanthum

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