In Vivo and in Vitro Exposure to PCB 153 Reduces Long-Term Potentiation

Hussain, Rifat J.; Győri, János; DeCaprio, Anthony P.; Carpenter, David O.

doi:10.2307/3434989

Cited by 7 publications

(15 citation statements)

References 38 publications

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“…In our study, CB-153 (a PCB congener with a non-planar structure) was the most abundant PCB congener in CSF and cerebellum GM. In rats, exposure to CB-153 during development causes reduction in learning ability, behavioral alterations, and reduced long-term potentiation (Holene et al, 1998;Hussain et al, 2000;Schantz et al, 1995).…”

Section: Pcb and Pcb Metabolitesmentioning

confidence: 99%

Organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and Atlantic white-sided dolphins from the western North Atlantic

Montie

Reddy

Gebbink

et al. 2009

Environmental Pollution

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Section: Pcb and Pcb Metabolitesmentioning

confidence: 99%

Organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and Atlantic white-sided dolphins from the western North Atlantic

Montie

Reddy

Gebbink

et al. 2009

Environmental Pollution

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“…As described above, LTP was measured no less than 1 hr after tetanic stimulation, then the I-O curves were again determined. Other experimental details have been presented in earlier publications (30,31).…”

Section: Electrophysiologymentioning

confidence: 99%

“…We (27,(29)(30)(31) and others (28,44,45) have shown that LTP is reduced by both lead and PCBs in certain brain areas, including the piriform cortex, hippocampal CA1 and dentate gyrus, and visual cortex. Hippocampal CA3 has been less studied, although Gutowski et al (46) report that they did not observe any effect of developmental exposure to lead on LTP at this synapse.…”

Section: Lead and Pcbs Have Similar Effects On Ltpmentioning

confidence: 99%

“…The lead concentrations in animals at the two ages with the different exposure paradigms were reported by Hussain et al (30). For in vivo PCB exposure, timed-pregnant virgin rats were fed PCB 153 (2,2′,4,4′,5,5′-hexachlorobiphenyl) applied daily on a sweet wafer from gestational day 7 through PD21, at four dose levels (0, 1.25, 5.0, and 20 mg/kg/day) as described by Hussain et al (31). Control studies and studies with acute application of lead or PCB 153 were conducted using brains of animals at the same age that were not exposed to either substance before brain slice preparation.…”

Section: Animalsmentioning

confidence: 99%

“…The brain was quickly removed into ice-cold Krebs-Ringer solution in which all NaCl was replaced by isosmotic sucrose, blocked, and slices of hippocampus were cut at 450 µm on a vibratome, as previously described (30,31). The slices were placed in normal, Na + -containing Krebs-Ringer for at least 1 hr before being mounted in the recording chamber.…”

Section: Electrophysiologymentioning

confidence: 99%

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Electrophysiologic and behavioral effects of perinatal and acute exposure of rats to lead and polychlorinated biphenyls.

Carpenter

Hussain

Berger

et al. 2002

Environ Health Perspect

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Lead and polychlorinated biphenyls (PCBs) both cause a reduction of intelligence quotient and behavioral abnormalities in exposed children that have features in common with attention deficit hyperactivity disorder. We have used rats as a model to study the effects of both perinatal and acute exposure to lead or PCBs in an effort to compare and understand the mechanisms of these nervous system decrements. Long-term potentiation (LTP) is an electrophysiologic measurement that correlates well with cognitive ability. We have determined the effects of chronic perinatal exposure to lead or PCB 153 as well as acute application of these substances to isolated brain slices, with recordings in two areas of the hippocampus, CA1 and CA3. Both substances, whether chronically or acutely applied, significantly reduced LTP in CA1 in animals at age 30 and 60 days. In CA3, they reduced LTP in 30-day animals but potentiated it in 60-day animals. Although neither lead nor PCB 153 alters baseline synaptic transmission at low stimulus strengths, at higher levels they induce changes in the same direction as those of LTP. These results show surprisingly similar actions of these quite different chemicals, and the similarity of effects on chronic and acute application indicates that effects are both pharmacologic and developmental. Behavioral studies of rats exposed to PCBs from contaminated fish show hyperactivity, impulsiveness, and increased frustration relative to unexposed controls. These results demonstrate that lead and PCBs have similar effects on synaptic plasticity and behavior and suggest that the compounds may act through a common mechanism. Key words: brain slice, extinction, frustration, hippocampus, hyperactivity, input-output curves, long-term potentiation, perinatal exposure.

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Hydroxylated PCB induces Ca²⁺ oscillations and alterations of membrane potential in cultured cortical cells

Londono

Shimokawa

Miyazaki

et al. 2009

J of Applied Toxicology

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Polychlorinated biphenyls (PCBs) are known as environmental pollutants that may cause adverse health effects. Although some congeners have been shown to affect brain development or function, the molecular mechanisms mediating their toxicity are not yet fully understood. Since signal transduction via intracellular Ca(2+) is crucial for neuronal development and plasticity, we investigated the effect of PCBs on Ca(2+) homeostasis and membrane potential in cultured mouse cortical cells. Acute exposure to hydroxylated PCB 106 [4(OH)-2',3,3',4',5'-pentachlorobiphenyl, OH-PCB 106, 0.1 microM] caused recurring Ca(2+) oscillations that were classified into three prototypes. Although extracellular Ca(2+) deprivation significantly reduced the oscillations, 54% of the cells still showed different patterns of oscillations or gradual increase in the intracellular Ca(2+) concentration, indicating possible involvement of multiple Ca(2+) channels in a cell-specific manner. Such a possibility was further confirmed by differential responses to several channel/receptor blockers, including nifedipine, ryanodine, xestospongine and tetrodotoxin. Although all chemicals had partial inhibition action in different subsets of neurons, nifedipine blocked the OH-PCB 106 action in the largest subpopulation of cells and with the greatest magnitude. Ryanodine also blocked the action with a similar magnitude, but in a smaller subpopulation of cells. Moreover, OH-PCB 106 induced depolarization of the plasma membrane in all the recorded cells. Taken together, our results indicate that OH-PCB 106 alters membrane potential as well as Ca(2+) dynamics in part by inducing extracellular influx and/or intracellular release of Ca(2+). These mechanisms may be responsible for their neurotoxicity.

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In Vivo and in Vitro Exposure to PCB 153 Reduces Long-Term Potentiation

Cited by 7 publications

References 38 publications

Organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and Atlantic white-sided dolphins from the western North Atlantic

Organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and Atlantic white-sided dolphins from the western North Atlantic

Electrophysiologic and behavioral effects of perinatal and acute exposure of rats to lead and polychlorinated biphenyls.

Hydroxylated PCB induces Ca²⁺ oscillations and alterations of membrane potential in cultured cortical cells

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In Vivo and in Vitro Exposure to PCB 153 Reduces Long-Term Potentiation

Cited by 7 publications

References 38 publications

Organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and Atlantic white-sided dolphins from the western North Atlantic

Organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and Atlantic white-sided dolphins from the western North Atlantic

Electrophysiologic and behavioral effects of perinatal and acute exposure of rats to lead and polychlorinated biphenyls.

Hydroxylated PCB induces Ca2+ oscillations and alterations of membrane potential in cultured cortical cells

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Product

Resources

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Hydroxylated PCB induces Ca²⁺ oscillations and alterations of membrane potential in cultured cortical cells