In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

Yang, Ning‐Sun; Burkholder, Joe; Roberts, Beth; Martinell, Brian; McCabe, Dennis E.

doi:10.1073/pnas.87.24.9568

Cited by 516 publications

(220 citation statements)

References 30 publications

Supporting

Mentioning

208

Contrasting

Unclassified

Order By: Relevance

“…It is highly desirable that an initial or pretherapeutic determination of the level of transgene expression be systematically evaluated under various standardized or relevant in vivo and/or in vitro conditions. 5,22,26,34,35 Different cell types, tissues/tumors can express the same transgenic CMV promoter with highly variable levels; and different subclones of CMV promoters perform very differently. This study thus may serve as an example for such a broad level and high diversity on transgene gene expression in various target tissue systems, and these may serve as useful information for future clinical applications.…”

Section: Optimization Of Concomitant/coordinated Expression Of Two Sumentioning

confidence: 99%

Molecular strategies for improving cytokine transgene expression in normal and malignant tissues

Turner

2003

Gene Ther

View full text Add to dashboard Cite

The augmentation and optimization of specific targeted transgene expression systems are important strategies for clinical research into gene therapy and DNA vaccination, due to safety considerations. In this study, we introduced 3 0 untranslated regions and transcriptional control modifications and direct tandem or combinational vector design strategies into a number of specific cytokine cDNA expression plasmids. The experiments were performed in parallel using both in vivo and in vitro transgene expression systems. In vivo studies were carried out using gene gun delivery of test vectors into mouse skin tissues. A combination of specific cell lines and fresh cell explants were used for in vitro and ex vivo transgene expression assay systems. The results from these comparative experiments demonstrated that a number of molecular biology manipulations can be readily adapted to define and significantly enhance the level or/and duration of transgene expression for a group of clinically relevant cytokine genes, with very similar effects for both in vivo and in vitro test systems. This cytokine transgene expression system may offer a favorable means for improving the efficiency of cytokine gene therapy and DNA vaccines in future clinical studies.

show abstract

Section: Optimization Of Concomitant/coordinated Expression Of Two Sumentioning

confidence: 99%

Molecular strategies for improving cytokine transgene expression in normal and malignant tissues

Turner

2003

Gene Ther

View full text Add to dashboard Cite

show abstract

“…101-104 The technique can be used to deliver DNA to liver, skin and muscle with as much as 20% of the cells expressing antigen within the field of immunization. 105 The plasmid exists episomally and generally does not integrate into the genome. Both DNA-based approaches have been shown to successfully induce both cellular and humoral immunity in many antigen systems.…”

Section: Dna-based Immunizationmentioning

confidence: 99%

The next wave of recombinant and synthetic anticancer vaccines

Irvine

Restifo

1995

Seminars in Cancer Biology

View full text Add to dashboard Cite

The identification of tumor-associated antigens (TAA) recognized by T lymphocytes makes the development of antigen-specific synthetic and recombinant vaccines possible. The expression of TAA within a recombinant vector increases control over the kinetics and quantity, the molecular form, and the subcellular location of the immunogen delivered. The next generation of antitumor vaccines employs cytokines and costimulatory molecules expressed in concert with TAA that are capable of augmenting the activation and proliferation of antitumor immune responses. The ultimate goal of these new strategies, the treatment of established cancer, is now being realized in animal models.Keywords tumor-associated antigen; recombinant vaccines; immunotherapy; recombinant fowlpox; recombinant adenovirus Elements of the cellular immune system clearly play a role in antitumor immunity. 1-4 The recent cloning of new TAA recognized by T lymphocytes allows for the development of recombinant and synthetic anticancer vaccines. Combining new techniques in molecular biology, virology and synthetic protein chemistry, specific vaccines can be developed that increase the control the immunotherapist has over the quantity and kinetics of TAA expression, the intracellular compartment into which the TAA are expressed and what cell types or tissues are used to express TAA in vivo. In addition to the classic concept of viral vaccination in which individuals are immunized against antigens prior to natural challenge with the pathogen, therapeutic cancer vaccination may also be used to induce or enhance antitumor immune reactions in patients who already have cancer. This review will discuss examples of current recombinant and synthetic vaccine strategies designed to enhance or elicit antitumor responses.

show abstract

“…These include projectile delivery (Yang et al, 1990), hydrodynamic delivery (Andrianaivo et al, 2004), electroporation (Mir et al, 1999), sonoporation (Bao et al, 1997), and magnetic forcemediated delivery Huth et al, 2004;Mah et al, 2002;Scherer et al, 2002). Magnetic force-mediated gene delivery, also termed "magnetofectionȍ, is related to the concept of magnetically targeted drug delivery (Meyers et al, 1963) and involves the application of a static magnetic field that guides magnetic particle (MP)-associated gene vectors to accumulate on the cell surface (Dobson, 2006;Mah et al, 2002;Scherer et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery

Pan

Guan

Yoo

et al. 2008

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Cationic lipid-coated magnetic nanoparticles (MPs) associated with transferrin were evaluated as gene transfer vectors in the presence of a static magnetic field. MPs were prepared by chemical precipitation and were surface-coated with cationic lipids, composed of DDAB/soy PC (60:40 mole/mole). These cationic MPs were then combined with polyethylenimine (PEI) condensed plasmid DNA, followed by transferrin. The resulting magnetic electrostatic complexes retained relatively compact particle size and showed complete DNA condensation. Their transfection activity in the presence of a static magnetic field was evaluated by luciferase and green fluorescent protein (GFP) reporter genes. The magnetic complexes exhibited up to 300-fold higher transfection activity compared to commonly used cationic liposomes or cationic polymer complexes, based on luciferase assay. The enhancement in transfection activity was maximized when the cells were exposed to the vectors for a relatively short period of time (15 min), or were treated in media containing 10% serum. Incorporation of transferrin further improved transfection efficiency of the cationic MPs. However, when cells were incubated for 4 h in serum-free media, magnetic and non-magnetic vectors showed similar transfection efficiencies. In conclusion, transferrin-associated cationic MPs are excellent gene transfer vectors that can mediate very rapid and efficient gene transfer in vitro in the presence of a magnetic field.

show abstract

In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

Cited by 516 publications

References 30 publications

Molecular strategies for improving cytokine transgene expression in normal and malignant tissues

Molecular strategies for improving cytokine transgene expression in normal and malignant tissues

The next wave of recombinant and synthetic anticancer vaccines

Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery

Contact Info

Product

Resources

About