Abstract:The study was aimed to trace out antihyperglycemic potentials of Nymphaea alba leaves using in vitro and in vivo approaches. In, in vitro study, determination of IC50 of Nymphaea alba extracts was done using α-amylase and α-glucosidase inhibition assay. In α-amylase, Nymphaea alba methanol extract (NAME) exhibited maximum inhibition 56.77±1.23% at 125µg/ml in comparison to 66.7±0.94 % of standard acarbose. In α-glucosidase, NAME exhibits 59.89±0.92, while standard acarbose showed 70.31±1.25 % inhibition. The i… Show more
“…The Korsmeyer-Peppas model also describes drug release from polymeric systems [ 52 , 53 ], and fitting of this model was also good, as shown by the obtained R 2 adjusted values. The n value, although more adapted to cylindrical shaped matrices [ 52 , 53 ], suggests that Fickian diffusion occurs in all patches, since n ~ 0.5 [ 57 ]. The Weibull model, adapted to describe different dissolution processes [ 53 ], is also useful for the comparison of drug release profiles from matrix systems [ 53 , 57 ].…”
Section: Resultsmentioning
confidence: 93%
“…Overall, this data suggest that this model cannot be applied to the triangular and grid-like gelatin-based patches, because their fragmentation was clear during the release test; this model do not mimic their dissolution profile.. The Korsmeyer-Peppas model also describes drug release from polymeric systems [ 52 , 53 ], and fitting of this model was also good, as shown by the obtained R 2 adjusted values. The n value, although more adapted to cylindrical shaped matrices [ 52 , 53 ], suggests that Fickian diffusion occurs in all patches, since n ~ 0.5 [ 57 ].…”
Section: Resultsmentioning
confidence: 96%
“…There are several in vitro methods which describe the overall release from drug-loaded vehicles, allowing a better prediction of the in vivo performance [ 52 , 53 ]. In general, a biodegradable polymeric matrix will release its content due to a diffusion, erosion, or degradation mechanism [ 54 – 56 ].…”
Cell-free based therapies, for example, the use of the cell secretome, have emerged as a promising alternative to conventional skin therapies using bioactive and, when combined with 3D printing technologies, allow the development of personalized dosage forms. This research work aimed to develop gelatin-based patches with controlled network topology via extrusion 3D printing, loaded with cell culture medium as a model of the secretome, and applicable as vehicles for topical delivery. Inks were optimized through rheological and printing assays, and the incorporation of medium had minor effects in printability. Regarding network topology, grid infills rendered more defined structures than the triangular layout, depicting clearer pores and pore area consistency. Release studies showed that filament spacing and infill pattern influenced the release of rhodamine B (model bioactive) and bovine serum albumin (model protein). Moreover, the grid patches (G-0.7/1/0.7), despite having around a seven-fold higher mean pore area than 0.7-mm triangular ones (T-0.7), showed a similar release profile, which can be linked to the network topology of the printed structures This work provided insight on employing (bio)printing in the production of carriers with reproducible and controlled pore area, able to incorporate cell-derived secretome and to be quickly tailored to the patient’s lesions.
Graphical Abstract
“…The Korsmeyer-Peppas model also describes drug release from polymeric systems [ 52 , 53 ], and fitting of this model was also good, as shown by the obtained R 2 adjusted values. The n value, although more adapted to cylindrical shaped matrices [ 52 , 53 ], suggests that Fickian diffusion occurs in all patches, since n ~ 0.5 [ 57 ]. The Weibull model, adapted to describe different dissolution processes [ 53 ], is also useful for the comparison of drug release profiles from matrix systems [ 53 , 57 ].…”
Section: Resultsmentioning
confidence: 93%
“…Overall, this data suggest that this model cannot be applied to the triangular and grid-like gelatin-based patches, because their fragmentation was clear during the release test; this model do not mimic their dissolution profile.. The Korsmeyer-Peppas model also describes drug release from polymeric systems [ 52 , 53 ], and fitting of this model was also good, as shown by the obtained R 2 adjusted values. The n value, although more adapted to cylindrical shaped matrices [ 52 , 53 ], suggests that Fickian diffusion occurs in all patches, since n ~ 0.5 [ 57 ].…”
Section: Resultsmentioning
confidence: 96%
“…There are several in vitro methods which describe the overall release from drug-loaded vehicles, allowing a better prediction of the in vivo performance [ 52 , 53 ]. In general, a biodegradable polymeric matrix will release its content due to a diffusion, erosion, or degradation mechanism [ 54 – 56 ].…”
Cell-free based therapies, for example, the use of the cell secretome, have emerged as a promising alternative to conventional skin therapies using bioactive and, when combined with 3D printing technologies, allow the development of personalized dosage forms. This research work aimed to develop gelatin-based patches with controlled network topology via extrusion 3D printing, loaded with cell culture medium as a model of the secretome, and applicable as vehicles for topical delivery. Inks were optimized through rheological and printing assays, and the incorporation of medium had minor effects in printability. Regarding network topology, grid infills rendered more defined structures than the triangular layout, depicting clearer pores and pore area consistency. Release studies showed that filament spacing and infill pattern influenced the release of rhodamine B (model bioactive) and bovine serum albumin (model protein). Moreover, the grid patches (G-0.7/1/0.7), despite having around a seven-fold higher mean pore area than 0.7-mm triangular ones (T-0.7), showed a similar release profile, which can be linked to the network topology of the printed structures This work provided insight on employing (bio)printing in the production of carriers with reproducible and controlled pore area, able to incorporate cell-derived secretome and to be quickly tailored to the patient’s lesions.
Graphical Abstract
“…In vitro release studies of CRC from NLC Drug release is the procedure by which a drug leaves a pharmaceutical formulation (to supply one or many pharmacological actions) and is subjected to absorption, distribution, metabolism and excretion. Drug release is described in various methods including immediate, modified, delayed, extended, controlled and pulsatile release [53]. Different kinetics models describe drug dissolution from immediate and modified release dosage forms such as zero-order, first-order, Higuchi and Hixson-Crowell.…”
Section: Figure 7 Antioxidant Activity Of Developed-nlc Versus Native...mentioning
This study focuses on comparing the ability of two kinds of lipid matricessolid lipids and vegetable oil associated or not with a lipid mediator (phenylalaninol oleamide, PO), for an efficient loading of Curcumin (CRC) into nanostructured lipid carriers (NLC). The Curcumin-loaded NLC were analysed in terms of particle size, physical stability, and encapsulation efficiency. By using the in vitro ABTS assay this research underlined the dominance of vegetable oils on the antioxidant properties of developed NLC formulations prepared with argan oil (AO) and linseed oil (LO). Another direction was to investigate the influence of the argan oil and linseed oil for a desired in vitro sustained release of CRC from the designed NLC formulations. Both antioxidant activity results and controlled release study highlighted the potential role of such formulation in normalizing the in vitro fate of poorly soluble Curcumin.
RezumatAcest studiu are ca obiectiv compararea capacității a două tipuri de matrice lipidice -lipide solide și ulei vegetal asociat sau nu cu un mediator lipidic (fenilalaninol oleamidă, PO), pentru o încărcare eficientă a curcuminei (CRC) în carrieri lipidici nanostructurați (NLC). NLC-urile încărcate cu curcumină au fost analizate în ceea ce privește dimensiunea particulelor, stabilitatea fizică și eficiența încapsulării. Prin utilizarea testului ABTS in vitro, această cercetare a subliniat dominanța uleiurilor vegetale asupra proprietăților antioxidante ale formulărilor NLC dezvoltate, preparate cu ulei de argan (AO) și ulei de in (LO). O altă direcție a fost investigarea influenței uleiului de argan și uleiului de in pentru o dorită eliberare susținută in vitro de CRC din formulările NLC proiectate. Atât rezultatele activității antioxidante, cât și studiul cu eliberare controlată au evidențiat rolul potențial al unei astfel de formulări în normalizarea profilului in vitro a curcuminei slab solubile.
1H-indol-2,3-dione (isatin) class of biologically active compounds have analgesic, anti-microbial, anti-inflammatory, anti-tubercular, anti-proliferative properties, and is also useful for the treatment of SARS-CoV. Schiff bases containing isatin moiety are known to have broad spectrum of biological activities like anti-viral, anti-tubercular, anti-fungal, and anti-bacterial. In this work, several Schiff base derivatives have been synthesized using two methods (synthetic and microwave) by reacting isatin with
o
-phenylenediamine. The synthesized compounds were structurally characterized and their
in-vivo
antimicrobial activity was tested against Gram-negative and Gram-positive bacteria using the inhibition zone method. Several newly synthesized isatin derivatives were found effective as antimicrobial agents and showed good potency (compounds
3c
,
3d
,
6a
,
6b
,
6d
). Compound
3c
displayed higher antimicrobial activity than standard drug (Amoxicillin) against
Staphylococcus aureus
at higher concentration (16 μg/mL) and against
Escherichia coli
at lower concentration (1 μg/mL).
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