In vivo and in vitro studies on the pathway of modification of mussel pyruvate kinase

Holwerda, D.A.; Veldhuizen-Tsoerkan, M.B.; Veenhof, P. R.; Evers, Ellen

doi:10.1016/0305-0491(89)90295-2

Cited by 13 publications

(13 citation statements)

References 16 publications

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“…Furthermore, PK controls the flux of phosphoenolpyruvate (PEP) to succinate during anaerobiosis. In response to anoxia the relative activity of PK (V o /V max ) declines in the adductor muscle and mantle of bivalve molluscs, indicating a reduction of PK capacity and a shift towards a less active form during metabolic depression (Holwerda et al, 1984;Holwerda et al, 1989). In addition, low levels of relative PK activity mirror low rates of pyruvate supply to aerobic metabolism and, thus, low rates of overall energy turnover.…”

Section: Discussionmentioning

confidence: 99%

“…The ratio V o /V max , which reflects the relative activity of PK, was then calculated to determine metabolic depression during acclimation to high temperature. The ratio V o /V max was found to decline in the adductor muscle and mantle of bivalve molluscs during anoxia indicating a shift of PK toward a less-active form during metabolic depression (Holwerda et al, 1984;Holwerda et al, 1989).…”

Section: Mmollmentioning

confidence: 99%

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Metabolic and molecular stress responses of sublittoral bearded horse musselModiolus barbatusto warming sea water: implications for vertical zonation

Anestis

Pörtner

Lazou

et al. 2008

Journal of Experimental Biology

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SUMMARYThe present study set out to investigate the thermal limits of the Mediterranean bivalve Modiolus barbatus, acclimated to various temperatures, and includes a comparison of laboratory determined limits with its temperature-dependent restriction to deeper water layers in its natural habitat. Thermal responses and limits were determined by integrating information from various levels of biological organization, including the expression of Hsp70 and Hsp90, the phosphorylation of stress-activated protein kinases, p38 mitogen-activated protein kinase (p38 MAPK) and cJun-N-terminal kinases (JNKs) as well as metabolic adjustments. The latter were assessed by examining temperature effects on the activity of the key glycolytic enzyme pyruvate kinase (PK). The expression of Hsp70 and Hsp90 was activated when mussels were acclimated to temperatures above 20°C. Increased phosphorylation of p38 MAPK and JNKs at about the same temperatures indicate activation of MAPK signaling cascades and their potential involvement in the induction of Hsp genes. As indicated by the activity of PK, Modiolus barbatus maintains some aerobic capacity when acclimated to temperatures up to 24°C, while further warming probably caused metabolic depression and a shift from aerobic to anaerobic metabolism. An increase in mortality occurred in parallel, during acclimation to temperatures above 24°C. Our results indicate that both the biochemical stress indicators and metabolic status respond in parallel once hypoxemia becomes extreme. Comparison with our previous study of thermal limits and vertical distribution in M. galloprovincialis dwelling in shallow waters emphasizes the relevance of maintained aerobic scope over that of passive tolerance for permanent vertical zonation at higher temperatures in the field. These findings and conclusions are in line with the concept of oxygen and capacity limited thermal tolerance and the associated systemic to molecular hierarchy of thermal limitation.

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Section: Discussionmentioning

confidence: 99%

Section: Mmollmentioning

confidence: 99%

Metabolic and molecular stress responses of sublittoral bearded horse musselModiolus barbatusto warming sea water: implications for vertical zonation

Anestis

Pörtner

Lazou

et al. 2008

Journal of Experimental Biology

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confidence: 99%

“…In mollusc tissues, pyruvate kinase activity is controlled by FDP, ATP and Lalanine, as well as ATP, cAMP and Ca 2+ (Holwerda et al, 1989). In addition, molluscan pyruvate kinase is regulated by the phosphorylation/dephosphorylation process (Marie et al, 1979;Siebenaller 1979;Kiener and Westhead, 1980;Nakashima et al, 1982;Hakim et al, 1984;Holwerda et al, 1989;Carillo et al, 2001). Moreover, there are many physiological factors such as temperature, anaerobic conditions, tidal cycle, seasonal and ion concentration variations that can alter pyruvate kinase activity in molluscs (Cortesi and Carpene, 1981;Carpene et al, 1984;Hakim et al, 1984;Cortesi et al, 1985;Ibarguren et al, 1990;Simpfendorfer et al, 1997).…”

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confidence: 99%

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Dailianis

Kaloyianni

2004

Journal of Experimental Biology

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SUMMARYThe present study investigates the transduction pathway mediated by cadmium in isolated digestive gland cells of mussel Mytilus galloprovincialis. The effects of cadmium treatment on a key glycolytic enzyme, pyruvate kinase (PK), and on Na+/H+ exchanger activity were examined. Cadmium (50 μmol l–1) caused a significant elevation of intracellular pH (pHi) and a rise (176%) of Na influx relative to control values. The amiloride analogue, EIPA (20 nmol l–1), a Na+/H+ exchanger blocker,together with cadmium, significantly reduced the effect of treatment by cadmium alone on both Na+ influx and pHi. In addition, PK activity was significantly increased after treatment with cadmium. PK activity was inhibited after treatment of cells with amiloride or EIPA together with cadmium. Moreover, phorbol-ester (PMA), a potent activator of protein kinase C(PKC), caused a significant rise in both pHi and PK activity, while staurosporine or calphostin C reversed both events. Adrenaline, isoprenaline and phenylephrine alone or together with cadmium also significantly increased the pHi and PK activity of isolated digestive gland cells. The latter effectors in combination with cadmium showed a synergistic effect on pHi and PK. These responses seem to be blocked by propranolol, metoprolol and prazosin. Our findings suggest a hormone-like effect of cadmium on digestive gland cells. The signal transduction pathway induced by cadmium involves the stimulation of PK, PKC and Na+/H+ exchanger in isolated digestive gland cells of Mytilus galloprovincialis.

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“…The oxaloacetate formed is reduced in mitochondria to malate and this reduction regulates a coenzyme redox balance in the cytoplasm. The regulation of this branchpoint is provided by the reversible phosphorylation of PK which in the muscles of mussel is an allosteric enzyme (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Modification of pyruvate kinase and lactate dehydrogenase in foot muscle of the sea mussel Mytilus galloprovincialis under anaerobiosis and recovery

Lushchak

Bahnjukova²,

Spichenkov³

1997

Braz J Med Biol Res

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The modification of pyruvate kinase (PK) and lactate dehydrogenase (LDH) activity in foot muscle of the mussel Mytilus galloprovincialis during exposure to air and recovery in water was investigated. In the course of exposure to air, the activity of these enzymes measured at high and low substrate concentrations showed successive increases and decreases. Returning the mussels to water after exposure to air affected enzyme activity in a manner similar to anaerobiosis. When measuring at saturated concentrations of substrates and substrate and coenzyme for PK and LDH, respectively, the maximum activation of PK (37%) was observed at 4 h of animal exposure to air, and for LDH (67%) at 6 h exposure to air. During 24 h of exposure of animals to air, PK activity practically reached the stock level, while LDH was still activated (148%). The change in lactate dehydrogenase activity in mussel muscle during anoxia and recovery is described here for the first time. Variation in pyruvate kinase activity during exposure to air and recovery is linked to the alteration of half-maximal saturation constants and maximal velocity for both substrates. The possible role of reversible phosphorylation in the regulation of pyruvate kinase and lactate dehydrogenase properties is discussed.

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In vivo and in vitro studies on the pathway of modification of mussel pyruvate kinase

Cited by 13 publications

References 16 publications

Metabolic and molecular stress responses of sublittoral bearded horse musselModiolus barbatusto warming sea water: implications for vertical zonation

Metabolic and molecular stress responses of sublittoral bearded horse musselModiolus barbatusto warming sea water: implications for vertical zonation

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Modification of pyruvate kinase and lactate dehydrogenase in foot muscle of the sea mussel Mytilus galloprovincialis under anaerobiosis and recovery

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