In Vivo and in Vitro Phytochemical Studies of Arnica Montana Species From Romanian Eastern Carpathians

Stefanache, Camelia Paula; Danila, D.; Necula, Radu; Gille, Elvira; Vantu, Smaranda

doi:10.17660/actahortic.2012.955.19

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plant deficiency stresses the need of elaboration of effective propagation methods. Several protocols have been reported on in-vitro multiplication of A. montana on agar gelled medium differing by their efficiency; however, the propagation rate needs to be improved [3][4][5] . The conventional in-vitro propagation method on agar medium requires a considerable amount of handwork: shoots separation and regular subculturing on fresh medium every 4 to 6 weeks 6 .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2014

IJPSR

View full text Add to dashboard Cite

Arnica montana L. (Asteraceae) is a valuable medicinal plant species, endemic to Europe, which is threatened in many countries due to its overharvesting. The increasing market demand requires development of an effective method for A. montana rapid propagation, offering the possibility for its field cultivation as an alternative to plant gathering from nature. Three invitro culture systems were compared to determine the best shoot multiplication of A. montana: agar-gelled medium, static liquid medium and Temporary Immersion System (TIS RITA ® ). Murashige and Skoog (MS) nutrient medium supplemented with 1 mg/l BA and 0.1 mg/l IAA was used in all experiments. The highest micropropagation rate (18.2 shoots/explant, for 5 weeks) was obtained via TIS culture. Besides, these plants showed higher sesqiuterpene lactones content than those derived from the other tested systems. The shoots were successfully in -vitro rooted and ex-vitro adapted. The elaborated method for mass multiplication of A. montana allows significant enhancement of the invitro process and could be applied to produce planting material, thus contributing to the conservation of the species as well. QUICK RESPONSE CODE

show abstract

Section: Introductionmentioning

confidence: 99%

Untitled

2014

IJPSR

View full text Add to dashboard Cite

show abstract

In Vivo and in Vitro Phytochemical Studies of Arnica Montana Species From Romanian Eastern Carpathians

Cited by 1 publication

References 0 publications

Untitled

Untitled

Contact Info

Product

Resources

About