In vivo and in vitro penetration of vitamins into human red blood cells

Sorrell, Michael F.; Frank, Oscar; Aquino, Hermes; Thomson, Allan D.; Baker, Herman

doi:10.1093/ajcn/24.8.924

Cited by 13 publications

(3 citation statements)

References 13 publications

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“…Absorption of selected vitamins common in yeast was estimated for all subjects after they had ingested 35 grams of dried brewer's yeast suspended in water; the yeast was ingested within 5 minutes. Responses to folates of Lacto‐bacillus casei (ATCC 7469), Streptococcus faecium (formerly S. faecalis) (ATCC 8043), and Pediococcus cerevisiae (ATCC 8081) differentiated folates (11) in yeast before and after decon‐jugation (deglutamylation) to the monoglutamate with hog‐kidney extract (12, 13). Lactobacillus casei responds to oxidized and reduced folylmono‐ to folyltriglutamates and N 5 ‐methyltetrahydrofolate; P. cerevisiae only to reduced folylmono‐ to folyltriglutamates; and S. faecium to oxidized and reduced folylmonoglutamates.…”

Section: Methodsmentioning

confidence: 99%

Severe Impairment of Dietary Folate Utilization in the Elderly

Baker¹,

Jaslow²,

Frank³

1978

J American Geriatrics Society

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Absorption of folates, vitamin B6, pantothenate, and riboflavin from a natural food source (yeast) and from synthetic folylmonoglutamate was studied in 24 elderly subjects (age range, 73 to 101 years) and in 12 healthy younger subjects (age range, 24 to 42 years). All subjects absorbed riboflavin, vitamin B6, and pantothenate from yeast. Ingested folypolyglutamates (the preponderant folates in yeast) proved to be a very poor source of folates for the elderly subjects, whereas synthetic folylmonoglutamate served as a good source. In the younger subjects, yeast was a significant folate source. It is suggested that the folate deficits so common in the elderly are caused by impaired ability to obtain folate from ingested foods. The resultant folate deficit may induce changes in the epithelial structure and enzyme secretion of the small bowel, which may further exacerbate folate malabsorption.

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Section: Methodsmentioning

confidence: 99%

Severe Impairment of Dietary Folate Utilization in the Elderly

Baker¹,

Jaslow²,

Frank³

1978

J American Geriatrics Society

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“…THF is further methylated into 5-Me-THF, and this methylation allows it to penetrate the cell membrane of the small intestine . Further, 5-Me-THF can penetrate the cell membrane of each tissue from blood . Within a cell, 5-Me-THF is the starting material for folate metabolism .…”

Section: Discussionmentioning

confidence: 99%

“…31 Further, 5-Me-THF can penetrate the cell membrane of each tissue from blood. 32 Within a cell, 5-Me-THF is the starting material for folate metabolism. 33 Therefore, conversion to 5-Me-THF is important for absorption by our body.…”

Section: ■ Discussionmentioning

confidence: 99%

Folate Biofortification in Hydroponically Cultivated Spinach by the Addition of Phenylalanine

Watanabe

Ohtani

Tatsukami

et al. 2017

J. Agric. Food Chem.

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Folate is an important vitamin mainly ingested from vegetables, and folate deficiency causes various health problems. Recently, several studies demonstrated folate biofortification in plants or food crops by metabolic engineering through genetic modifications. However, the production and sales of genetically modified foods are under strict regulation. Here, we developed a new approach to achieve folate biofortification in spinach (Spinacia oleracea) without genetic modification. We hydroponically cultivated spinach with the addition of three candidate compounds expected to fortify folate. As a result of liquid chromatography tandem mass spectrometry analysis, we found that the addition of phenylalanine increased the folate content up to 2.0-fold (306 μg in 100 g of fresh spinach), representing 76.5% of the recommended daily allowance for adults. By measuring the intermediates of folate biosynthesis, we revealed that phenylalanine activated folate biosynthesis in spinach by increasing the levels of pteridine and p-aminobenzoic acid. Our approach is a promising and practical approach to cultivate nutrient-enriched vegetables.

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Kinetics of thiamin and thiamin phosphate esters in human blood, plasma and urine after 50 mg intravenously or orally

Tallaksen¹,

Sande

Bøhmer³

et al. 1993

Eur J Clin Pharmacol

108

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The concentrations of thiamin and thiamin monophosphate and diphosphate in plasma and whole blood samples were assessed in six healthy subjects for 12 h and in urine for 24 h following an IV and PO bolus dose of 50 mg thiamin HCl. Unphosphorylated thiamin increased rapidly in plasma after IV administration and then decreased to its initial value within 12 h in all but one subject; the half-life was 96 min. Thiamin mono and -diphosphate increased moderately (56%), and decreased slowly; the half-life of diphosphate was 664 min. Within 24 h, 53% of the administered dose was recovered in the urine, indicating a restricted distribution. After oral administration, the peak thiamin concentration in plasma was reached after 53 min and the concentration then had increased to 179% of its initial value. The elimination half-life was 154 min, and only 2.5% of the given dose was recovered in the urine. The relative bioavailability of thiamin was 5.3%. A moderate amount of the administered thiamin was stored in blood. Other body tissues must play an important part, therefore, in the distribution of thiamin.

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In vivo and in vitro penetration of vitamins into human red blood cells

Cited by 13 publications

References 13 publications

Severe Impairment of Dietary Folate Utilization in the Elderly

Severe Impairment of Dietary Folate Utilization in the Elderly

Folate Biofortification in Hydroponically Cultivated Spinach by the Addition of Phenylalanine

Kinetics of thiamin and thiamin phosphate esters in human blood, plasma and urine after 50 mg intravenously or orally

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