In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus

Pakingking, Rolando V.; Okinaka, Yasushi; Mori, Koichiro; Arimoto, Masao; Muroga, Kiyokuni; Nakai, Toshiko

doi:10.1016/j.fsi.2003.10.005

Cited by 38 publications

(28 citation statements)

References 29 publications

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“…The Mx gene in fish was first detected in 1989 15 and then seven forms of Mx genes were found in zebrafish 16 , three forms in rainbow trout 17 , two forms in crucian carp (Carassius auratus) 18 and a single form in Japanese flounder (Paralichthys olivaceus) 19 and Pufferfish 20 . However, the antiviral property of fish Mx proteins had been demonstrated only in Japanese flounder and Atlantic salmon against fish rhabdoviruses, infectious pancreatic necrosis virus, and viral haemorrhagic septicaemia virus 21,22,23 . Interferon regulatory factors (IRFs) were transcription factors which bound to the ISREs （ Interferon-stimulated response element） and mainly regulated the expression of type-I IFN gene, interferon stimulated genes (ISGs) and other cytokines 24,25 .…”

Section: Introductionmentioning

confidence: 99%

Expression analysis of three immune genes Interferon-gamma, Mx and Interferon regulatory factor-1 of Japanese flounder ( Paralichthys olivaceus )

Zhang

Qiu

Liu

et al. 2017

Braz. arch. biol. technol.

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IFN-γ (Interferon-gamma), Mx and IRF-1 (Interferon regulatory factor-1) are main immune-related genes and they play important roles in the innate immune system of vertebrates. In this study, the expression level of the three immune-related genes in twelve tissues of normal adult Japanese flounder was detected using semi-quantitative RT-PCR. Thirteen time points (3h, 6h, 9h, 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, 108h, 120h)

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Section: Introductionmentioning

confidence: 99%

Expression analysis of three immune genes Interferon-gamma, Mx and Interferon regulatory factor-1 of Japanese flounder ( Paralichthys olivaceus )

Zhang

Qiu

Liu

et al. 2017

Braz. arch. biol. technol.

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“…We previously demonstrated through dual-infection experiments with Japanese flounder Paralichthys olivaceus that primary infection with a non-lethal aquabirnavirus (ABV) could create a resistant state in fish against the subsequent infection with a lethal viral hemorrhagic septicemia virus (VHSV) (Pakingking et al, 2003(Pakingking et al, , 2004. The nonspecific protection lasted for at least 14 days post-ABV infection with the maximal pro tection occurring within 7 days post-ABV infection.…”

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confidence: 99%

“…RGNNV was propagated (MOl: RGNNV and ABV infective titers in dual-infected fish were quantified following a protocol adapted from our previous study (Pakingking et al, 2004). Briefly, RGNNV titration was carried out in 96-well plates seeded with E-11 cells after the sample homogenate was treated with a diluted rabbit antiserum (1:100) against yellowtail ascites virus (YTAV) to neutralize ABV infectivity.…”

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confidence: 99%

Aquabirnavirus-induced Protection of Marine Fish against Piscine Nodavirus Infection

et al. 2005

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ABSTRACT--Experimental dual-infections with a non-lethal aquabirnavirus (ABV) and a lethal betanodavirus (redspotted grouper nervous necrosis virus: RGNNV) were carried out in Japanese flounder Paralichthys olivaceus and sevenband grouper Epinephelus septemfasciatus.In the dual-infection group, ABV was intramuscularly (IM) injected into fish seven days before the IM-injec tion with RGNNV. In the experiments with flounder, a high expression of an Mx gene, a molecular marker for type I interferon(s) (IFN) production, occurred in the head kidneys and brains at Day 7 post-ABV injection. Although no mortality was found not only in the dual-infected group but also in the single infection group with RGNNV (control group), the infective titers of RGNNV in the tissues of the dual-infected group were significantly lower at any sampling times than those in the control group. In the experiments with grouper, the preceding ABV infection resulted in complete protec tion against RGNNV infection. The infective titers of RGNNV in the tissues were also lower in the dual-infected group than in the control group throughout the experiments, and finally the virus dis appeared from the head kidneys and brains of the dual-infected group at Day 14 and Day 56 post injections, respectively. These results suggest that an ABV-induced IFN(s) effectively suppresses the progression of secondary betanodavirus infection.

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“…Mx induction by fish viruses has been reported in many cases, including channel catfish virus (CCV) in catfish [22], nodavirus and iridovirus in grouper [23], infectious hematopoietic necrosis virus (IHNV) in rainbow trout [16], IPNV in Atlantic halibut [18], hirame rhabdovirus (HIRRV) or aquabirnavirus in Japanese flounder [19,40] in vivo, and nodavirus in SAF-1 cells [21] in vitro. In the present study, we found that cBB cells were sensitive to nodavirus, birnavirus and iridovirus infection.…”

Section: Discussionmentioning

confidence: 99%

Cloning and analysis of antiviral activity of a barramundi (Lates calcarifer) Mx gene☆

Chi

2007

Fish & Shellfish Immunology

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We obtained a full-length cDNA clone for the Mx gene of barramundi (Lates calcarifer), using RACE (rapid amplification of cDNA ends) polymerase chain reaction ( C was higher than that at 20 C. Moreover, Mx gene expression was also induced by viral infection, including fish nodavirus, birnavirus, and iridovirus. Among these, nodavirus was a stronger inducer than the other two viruses. Using an antiviral activity assay, we revealed that poly I:C-transfected cBB cells had antiviral activity against fish nodavirus and birnavirus, but not iridovirus. Furthermore, the replication of nodavirus and birnavirus could be restored after the expression of Mx gene was down-regulated by siRNA. Therefore, these results indicated that the expression of barramundi Mx gene was able to inhibit the proliferation of fish nodavirus and birnavirus.

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In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus

Cited by 38 publications

References 29 publications

Expression analysis of three immune genes Interferon-gamma, Mx and Interferon regulatory factor-1 of Japanese flounder ( Paralichthys olivaceus )

Expression analysis of three immune genes Interferon-gamma, Mx and Interferon regulatory factor-1 of Japanese flounder ( Paralichthys olivaceus )

Aquabirnavirus-induced Protection of Marine Fish against Piscine Nodavirus Infection

Cloning and analysis of antiviral activity of a barramundi (Lates calcarifer) Mx gene☆

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