In vivo and in vitro effects of dexamethasone on leukocyte migration in the rat adjuvant arthritis model

Thieme, Thomas R.; Mirkovich, Anna; Maloney, P. J.; Goodwin, David A.

doi:10.1007/bf00917308

Cited by 9 publications

(2 citation statements)

References 8 publications

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“…Cortisone acetate, for example, augments PMN accumulation in the livers of Listeria-infected mice (34). Dexamethasone enhances PMN migration in vitro but has the opposite effect on macrophages (46).…”

Section: Days After Intraperitoneal Infectionmentioning

confidence: 99%

Influence of steroidal and nonsteroidal sex hormones on host resistance in mice: increased susceptibility to Listeria monocytogenes after exposure to estrogenic hormones

Pung¹,

Luster²,

Hayes³

et al. 1984

Infect Immun

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Subchronic exposure to pharmacological levels of estrogenic compounds, including 1713-estradiol, diethylstilbestrol, and a-dienestrol, significantly increased the mortality of B6C3F, female mice after Listeria infection. Compounds with little estrogenic activity, including 5a-dihydrotestosterone, progesterone, zearalenol, and corticosterone, did not alter Listeria-related mortality. Estrogen-induced alterations in resistance were inhibited by both adult thymectomy and the estrogen antagonist tamoxifen. Estrogen exposure depressed the accumulation of monocytes and lymphocytes at infective foci. Significantly elevated numbers of bacteria were observed in infective foci of estrogen-treated mice later in the infection when bacteria were nearly eliminated from untreated animals. These results indicate that estrogen-induced suppression of Listeria immunity is partially mediated by the thymus, probably through receptor events which may ultimately suppress the activation of T cell-dependent defense mechanisms. This may be partially reflected by the inability of estrogenexposed mice to eliminate Listeria cells or to accumulate mononuclear effector cells at infective foci at the same rate as controls.

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Section: Days After Intraperitoneal Infectionmentioning

confidence: 99%

Influence of steroidal and nonsteroidal sex hormones on host resistance in mice: increased susceptibility to Listeria monocytogenes after exposure to estrogenic hormones

Pung¹,

Luster²,

Hayes³

et al. 1984

Infect Immun

View full text Add to dashboard Cite

show abstract

“…51 The overall increased proteolytic activity caused during the inflammatory response may affect the dialysis probes by degrading or destroying different probe components, especially the tubing connections that are glued. The reduction in the inflam- Other studies have demonstrated that the monocyte infiltration at the implant site is reduced by releasing dexamethasone, 52,53 but no evidence was provided regarding the variation of capsule thickness or the formation of collagen or blood capillaries. With the exception of day 7 and 10 VB 12 EE%, the calibration of implanted microdialysis probes was not altered as compared to controls when locally infusing dex-phos.…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Foreign Body Reaction for Implants in the Subcutaneous Space: Microdialysis Probes as Localized Drug Delivery/Sampling Devices

Mou

Lennartz

Loegering

et al. 2011

J Diabetes Sci Technol

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Modulation of the foreign body reaction is considered to be an important step toward creation of implanted sensors with reliable long-term performance. In this work, microdialysis probes were implanted into the subcutaneous space of Sprague-Dawley rats. The probe performance was evaluated by comparing collected endogenous glucose concentrations with internal standard calibration (2-deoxyglucose, antipyrine, and vitamin B12). Probes were tested until failure, which for this work was defined as loss of fluid flow. In order to determine the effect of fibrous capsule formation on probe function, monocyte chemoattractant protein-1/CC chemokine ligand 2 (MCP-1/CCL2) was delivered locally via the probe to increase capsule thickness and dexamethasone 21-phosphate was delivered to reduce capsule thickness. Probes delivering MCP-1 had a capsule that was twice the thickness (500-600 μm) of control probes (200-225 μm) and typically failed 2 days earlier than control probes. Probes delivering dexamethasone 21-phosphate had more fragile capsules and the probes typically failed 2 days later than controls. Unexpectedly, extraction efficiency and collected glucose concentrations exhibited minor differences between groups. This is an interesting result in that the foreign body capsule formation was related to the duration of probe function but did not consistently relate to probe calibration.

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Mechanism by which methylprednisolone inhibits acute immune complex-induced changes in vascular permeability

et al. 1986

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Intravital microscopy was used to quantitate protein leakage which resulted from the deposition of immune complexes in the vasculature of the rat cremaster muscle. Immune complex deposition was initiated by the addition of 80 micrograms/ml of ovalbumin to the bath surrounding the muscle, followed by the intravenous administration of antiovalbumin. Administration of 25 mg/kg of antiovalbumin produced significant leakage of protein from the third-order venules, while 7.5 and 2.5 mg/kg had no effect. Administration of methylprednisolone (MP), 30 mg/kg, 1 h prior to the deposition of immune complexes significantly inhibited protein leakage. In separate experiments, MP inhibited intradermal edema formation and protein exudation induced in rats by histamine, platelet activating factor, or C5a. However, MP had no effect on protein exudation or edema produced by xanthine oxidase or glucose oxidase. Intravenous administration of MP inhibited the ability of polymorphonuclear leukocytes (PMNs) to phagocytize bacteria, but failed to alter hydrogen peroxide production. These results suggest that MP prevents acute changes in vascular permeability following immune complex deposition by inhibiting the effects of soluble mediators of edema on vascular endothelium and by inhibiting PMN phagocytosis.

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In vivo and in vitro effects of dexamethasone on leukocyte migration in the rat adjuvant arthritis model

Cited by 9 publications

References 8 publications

Influence of steroidal and nonsteroidal sex hormones on host resistance in mice: increased susceptibility to Listeria monocytogenes after exposure to estrogenic hormones

Influence of steroidal and nonsteroidal sex hormones on host resistance in mice: increased susceptibility to Listeria monocytogenes after exposure to estrogenic hormones

Modulation of the Foreign Body Reaction for Implants in the Subcutaneous Space: Microdialysis Probes as Localized Drug Delivery/Sampling Devices

Mechanism by which methylprednisolone inhibits acute immune complex-induced changes in vascular permeability

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