In vivo and in vitro Patch-clamp Recording Analysis of the Process of Sensory Transmission in the Spinal Cord and Sensory Cortex

Yoshimura, Michihiro; Doi, Atsushi; Mizuno, Masaharu; Furue, Hidemasa; Katafuchi, Toshihiko

doi:10.2114/jpa.24.93

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…Following the successful application of patch-clamp recording in vivo in other systems (Covey, Kauer, & Casseday, 1996; C. I. Moore & Nelson, 1998), use of whole-cell recording techniques in the nociceptive systems of the spinal cord of the rat in vivo was reported (Furue, Narikawa, Kumamoto, & Yoshimura, 1999; Graham, Brichta, & Callister, 2004; Light & Willcockson, 1999; Weng & Dougherty, 2002; Yoshimura, Doi, Mizuno, Furue, & Katafuchi, 2005). The obvious advantages of this technique include better control over the electrical properties of the neuron, a more robust technique than sharp electrode intracellular recording, the ability to observe single channel activity in the native milieu, and easier control of both intracellular and extracellular medium for drug application.…”

Section: The Neuroscience Of Painmentioning

confidence: 99%

The biopsychosocial approach to chronic pain: Scientific advances and future directions.

Gatchel¹,

Peng²,

Peters³

et al. 2007

Psychological Bulletin

2,599

1,903

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The prevalence and cost of chronic pain is a major physical and mental health care problem in the United States today. As a result, there has been a recent explosion of research on chronic pain, with significant advances in better understanding its etiology, assessment and treatment. The purpose of the present article is to provide a review of the most noteworthy developments in the field. The biopsychosocial model is now widely accepted as the most heuristic approach to chronic pain. With this model in mind, a review of the basic neuroscience processes of pain (the bio part of biopsychosocial), as well as the psychosocial factors is presented. This spans research on how psychological and social factors can interact with brain processes to influence health and illness, to the development of new technologies, such as brain imaging, that provide new insights into brain-pain mechanisms.KEY WORDS: biopsychosocial; chronic pain; neuroscience of pain; pain and cognition; pain and emotion Biopsychosocial Approach to Chronic Pain 3The Biopsychosocial Approach to Chronic Pain: Scientific Advances and Future Directions During the past decade, there has been an explosion of research on chronic pain, with significant advances in understanding its etiology, assessment and treatment (Gatchel, 2004; Turk & Monarch, 2002). This research has important health care implications Epidemiological research has shown that chronic pain (loosely defined as prolonged and persistant pain of at least 3-months duration) and chronic recurrent pain (recurrent episodes of pain interspersed with pain free periods extending over months or years) affects 10% -20% of adults in the general population (Blyth et al., 2001; Gureje, Von Korff, Simon & Gater, 1998; Vehaak, Kerssens, Dekker, Sorbi & Bensing, 1998). For example, in a large-scale epidemiological study, Von Korff, Crane, Lane et al. (2005) estimated a 19% prevalence for chronic spinal pain (neck and back) in the U.S. in the previous year, and a 29% lifetime rate. The American Academy of Pain Management (2003) asserted that approximately 57% of all adult Americans reported experiencing recurrent or chronic pain in the past year. About 62% of those individuals reporting being in pain for more than 1 year, and 40% noted that they were constantly in pain. Indeed, as Gatchel (2004) has summarized that pain is a pervasive medical problem: it affects over 50 million Americans and costs more than $70 billion annually in health care costs and lost productivity; it accounts for more than 80% of all physician visits. Moreover, chronic pain is often associated with major comorbid psychiatric disorders and emotional suffering.As the above factors attest, the prevalence and cost of chronic pain is a major physical and mental health care problem in the United States. Moreover, individuals 50 years of age and older are twice as likely to have been diagnosed with chronic pain (Gatchel, 2004;. Currently, there are approximately 35 million Americans aged 65 years or older, accounting for 12.4% of t...

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Section: The Neuroscience Of Painmentioning

confidence: 99%

The biopsychosocial approach to chronic pain: Scientific advances and future directions.

Gatchel¹,

Peng²,

Peters³

et al. 2007

Psychological Bulletin

2,599

1,903

View full text Add to dashboard Cite

show abstract

“…Previously, Yoshimura et al [42] reported that noxious pinch stimulation near a whisker pad did not produce any significant response in SI neurons located at 100 to 1000 μm from the surface of the cortex in urethane-anesthetized rats. In the present study, only 33% (21/63) of the SI neurons tested (at a depth of 750–900 μm) responded to noxious stimulation applied to the orofacial area.…”

Section: Discussionmentioning

confidence: 99%

In vivo Patch-Clamp Analysis of Response Properties of Rat Primary Somatosensory Cortical Neurons Responding to Noxious Stimulation of the Facial Skin

et al. 2010

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BackgroundAlthough it has been widely accepted that the primary somatosensory (SI) cortex plays an important role in pain perception, it still remains unclear how the nociceptive mechanisms of synaptic transmission occur at the single neuron level. The aim of the present study was to examine whether noxious stimulation applied to the orofacial area evokes the synaptic response of SI neurons in urethane-anesthetized rats using an in vivo patch-clamp technique.ResultsIn vivo whole-cell current-clamp recordings were performed in rat SI neurons (layers III-IV). Twenty-seven out of 63 neurons were identified in the mechanical receptive field of the orofacial area (36 neurons showed no receptive field) and they were classified as non-nociceptive (low-threshold mechanoreceptive; 6/27, 22%) and nociceptive neurons. Nociceptive neurons were further divided into wide-dynamic range neurons (3/27, 11%) and nociceptive-specific neurons (18/27, 67%). In the majority of these neurons, a proportion of the excitatory postsynaptic potentials (EPSPs) reached the threshold, and then generated random discharges of action potentials. Noxious mechanical stimuli applied to the receptive field elicited a discharge of action potentials on the barrage of EPSPs. In the case of noxious chemical stimulation applied as mustard oil to the orofacial area, the membrane potential shifted depolarization and the rate of spontaneous discharges gradually increased as did the noxious pinch-evoked discharge rates, which were usually associated with potentiated EPSP amplitudes.ConclusionsThe present study provides evidence that SI neurons in deep layers III-V respond to the temporal summation of EPSPs due to noxious mechanical and chemical stimulation applied to the orofacial area and that these neurons may contribute to the processing of nociceptive information, including hyperalgesia.

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“…This in vivo patch-clamp recording technique permits functional analyses of modality-dependent synaptic responses evoked by acupuncture. Therefore, the behavioral changes at the single-cell level can be explained with more certainty [47]. …”

Section: Study Of Acupuncture-induced Changes In the Central Nervomentioning

confidence: 99%

Patch Clamp: A Powerful Technique for Studying the Mechanism of Acupuncture

Zhang

2012

Evidence-Based Complementary and Alternative Medicine

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Cellular and molecular events can be investigated using electrophysiological techniques. In particular, the patch-clamp method provides detailed information. In addition, the patch-clamp technique has become a powerful method for investigating the mechanisms underlying the effects of acupuncture. In this paper, recent researches on how acupuncture might modulate electrophysiological responses in the central nervous system (CNS) and affect peripheral structures are reviewed.

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In vivo and in vitro Patch-clamp Recording Analysis of the Process of Sensory Transmission in the Spinal Cord and Sensory Cortex

Cited by 5 publications

References 14 publications

The biopsychosocial approach to chronic pain: Scientific advances and future directions.

The biopsychosocial approach to chronic pain: Scientific advances and future directions.

In vivo Patch-Clamp Analysis of Response Properties of Rat Primary Somatosensory Cortical Neurons Responding to Noxious Stimulation of the Facial Skin

Patch Clamp: A Powerful Technique for Studying the Mechanism of Acupuncture

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