In vivo and in vitro studies of a propolis-enriched silk fibroin-gelatin composite nanofiber wound dressing

Du, Pan; Chen, Xue; Chen, Yang; Li, Jin; Lu, Yichi; Li, Xiaoxiao; Hu, Kai; Chen, Junfeng; Lu, Guozhong

doi:10.1016/j.heliyon.2023.e13506

Cited by 18 publications

(8 citation statements)

References 62 publications

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“…Subsequent MTT assays showed about 100% cell viability in the test samples (KL, TN, and TS). The ISO 10993‐5 standard was previously explored for determining the cytotoxicity of silk biomaterials in various forms (hydrogels, 26,27 films, 5 nanofibrous mat, 28 etc. ), wherein the cell viability was found to be greater than 70%—which was the acceptable limit as per the said standard.…”

Section: Discussionmentioning

confidence: 99%

A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

Dan

Singh

et al. 2023

MedComm – Biomaterials and Applications

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The demand‐to‐supply gap, rejection rates, and the chances of infection associated with organ/tissue transplantation prompted researchers to find alternative solutions such as tissue engineering. Here, healthy cells are cultured over a biomaterial framework supplemented with growth factors to create bioartificial tissues. As a scaffolding biomaterial, silk fibroin (SF), a biopolymer obtained from Bombyx mori silk cocoons, offers unique properties. However, natural polymers, including SF, were criticized for preconceived source‐dependent batch‐to‐batch variations. Therefore, this study aims to prepare B. mori SF‐based films and investigate source‐dependent variations, if any. For this purpose, we have sourced silk cocoons from three geographical locations in India and processed them into films with a solvent‐casting approach. As a whole, our results indicate that there were slight variations in the morphological features in the raw cocoon stage; however, once processed, there were no significant differences in their topological, physical, chemical, optical, mechanical, or degradable properties with respect to the source. Further, all the films were found to be noncytotoxic and cytocompatible with corneal cells in vitro. Therefore, the study indicates no source‐dependent variations in biopolymers and suggested that SF from any source can be processed into biomaterials for potential biomedical applications.

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Section: Discussionmentioning

confidence: 99%

A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

Dan

Singh

et al. 2023

MedComm – Biomaterials and Applications

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“…It also significantly encourages L929 cell migration. In vivo tests confirmed that propolis nanofiber accelerates wound healing, and histological experiments further verified its pro-healing properties [ 123 ]. Propolis-based nanofiber patches were also reported to improve corneal microbial keratitis by providing antimicrobial activity against S. aureus and P. aeruginosa , common microorganisms causing corneal keratitis.…”

Section: Pharmacological Actions Of Nanopropolis In Various Diseases ...mentioning

confidence: 96%

New insights of propolis nanoformulation and its therapeutic potential in human diseases

Kustiawan,

Syaifie,

Siregar

et al. 2024

ADMET DMPK

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Background and purpose: Scientific research is crucial to develop therapies for various disease severity levels, as modern drugs cause side effects and are difficult to predict. Researchers are exploring herbal alternatives with fewer side effects, particularly propolis, which has been validated through in vitro, in vivo, and clinical studies. This will focus on scientific evidence and its supporting technology for developing new bioactive compounds for chronic diseases. Nanotechnology can improve the delivery and absorption of herbal medicines, which often have poor bioavailability due to their high molecular weight and solubility in water, particularly in oral medicines. This technology can enhance propolis's effects through multi-target therapy and reduce side effects. Experimental approach: All publications related to each section of this review were discovered using the search engines Google Scholar, Scopus, and Pubmed. This was only available for publication between 2013 and 2023. The selected publications were used as references in this review after being thoroughly studied. Key results: Evaluation of propolis active compounds, the classification of propolis nano formulations, design concepts, and mechanisms of action of propolis nano formulation. Additionally, the challenges and prospects for how these insights can be translated into clinical benefits are discussed. Conclusion: In the last ten years, a list of nanoformulation propolis has been reported. This review concludes the difficulties encountered in developing large-scale nanoformulations. To commercialize them, improvements in nano carrier synthesis, standardized evaluation methodology within the framework of strategy process improvement, and Good Manufacturing Practices would be required.

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“…147 Compared with other synthetic and natural polymers, SF has better biocompatibility, non-toxicity, biodegradability, resistance, and thermal stability. 148,149 In addition, SF has excellent toughness and is oen mixed with other polymers to improve the mechanical properties of nanobers. Khosravimelal prepared CS/SF electrospun nanobers containing cationic antimicrobial peptides as wound dressings.…”

Section: Polymer Used For Making Electrospun Nanofiber Burn Dressingsmentioning

confidence: 99%

Recent progress of electrospun nanofibers as burning dressings

Zhang,

Yang,

Gong

et al. 2024

RSC Adv.

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In vivo and in vitro studies of a propolis-enriched silk fibroin-gelatin composite nanofiber wound dressing

Cited by 18 publications

References 62 publications

A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

A study on source dependent batch to batch variations in silk fibroin films for potential applications in corneal tissue engineering

New insights of propolis nanoformulation and its therapeutic potential in human diseases

Recent progress of electrospun nanofibers as burning dressings

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