In vivo and in vitro biocompatibility study of novel microemulsion hybridized with bovine serum albumin as nanocarrier for drug delivery

Abstract

The present study aimed to synthesize triacetin-microemulsion (T-ME) and T-ME hybridized with bovine serum albumin nanoparticles (T-BSA-ME) having narrow particle size distribution and versatile carrier systems as a novel microemulsion system. The suggested ME system was characterized by Fourier Transform Infrared spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), and Atomic Force Microscopy (AFM). The physicochemical properties of microemulsion system including particle size, PDI and ζ-potential, refractive index, Conductivity, %Transmittance, pH, and rheological behavior were also evaluated. In vivo biocompatibility was done using Median Lethal Dose (LD 50) calculated and trialed to evaluate the acute toxicity. In Addition, hemolysis and leukocyte proliferation assay were characterized to evaluate in-vitro biocompatibility of the suggested MEs systems. Moreover, cytotoxicity of MEs systems was also investigated on HFF-2 and HEK-293 cells. The presence of BSA NPs as a macromolecular biomaterial hybridized with T-ME reduced the cytotoxicity. The properties of the suggested MEs system proposed the T-ME hybridized with BSA-NPs as a promising candidate for co-delivery and multifunctional biomedicine applications.

Novel Gel Microemulsion as Topical Drug Delivery System for Curcumin in Dermatocosmetics

“…To obtain information about the structural state, the LVM microemulsion was characterized by electrical conductivity measurements, DLS, in order to determine the size of the discontinuous phase droplets, and polarized light microscopy. The conductivity measurements provided a value of electrical conductivity of 228 μS/cm, which is characteristic for an O/W type microemulsion [ 30 , 31 , 32 , 33 ]. The DLS measurements showed that the discontinuous phase droplets had a uniform dimensional distribution and an average diameter of about 74.73 ± 6.06 nm ( Figure 1 ).…”

Biocompatibility of Biomaterials for Nanoencapsulation: Current Approaches

“…The in vivo and in vitro biocompatibility of a novel microemulsion hybridized with bovine serum albumin as a nanocarrier, in which Span ® 80 and Tween ® 80 were used as surfactants for drug delivery, was reported [ 304 ] The haemolytic activity of triacetin microemulsions (T-ME) and triacetin-bovine serum albumin microemulsions (T-BSA-ME) in different concentrations ranged between 6.32 ± 0.02 to 13.00 ± 0.12% and 9.09 ± 0.00 to 15.87 ± 0.00%, respectively. The changes in weight of the mice after 24 h and 7 days was approximately 1.58 ± 3.64% and 5.42 ± 1.78%, respectively.…”

pH DEPENDENT STRUCTURES OF BOVINE SERUM IN SOLUTION BY SMALL ANGLE NEUTRON SCATTERING

“…BSA has been widely used as drug delivery device because of its non-toxicity, biodegradability, nonimmunogenicity, water solubility, availability and its low cost, because large quantities of it can be readily purified from bovine blood [3]. In order to be used as drug delivery device, BSA has been bound with ligand complex [1] and polymer [4], or complexed with magnetic iron oxide [5].…”