In Vivo Anti-diabetic Effects of Aqueous Leaf Extracts of Rhoicissus tridentata in Alloxan Induced Diabetic Mice

Mukundi, Mwangi J; Mwaniki, Njagi En; Ngugi, Mathew Piero; Murugi, Njagi Joan; Daniel, Agyirifo S; Peter, Gathumbi K; Alice, Muchugi N

doi:10.4172/2329-6631.1000131

Cited by 5 publications

(1 citation statement)

References 16 publications

(13 reference statements)

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“…Rat blood samples were taken from the vein in the tail of rats 72 hours after induction (FPG 0) and on day 28 (FPG 28) (after rats were given bark extract and glibenclamide as standard). [21][22][23][24][25][26].…”

Section: Anti-diabetic Activity Test Of Sfsementioning

confidence: 99%

Anti-Diabetic Effect of Snake Fruit Skin Extract in Alloxan-Induced Wistar Rat

Marzuki

Girsang

Nasution

et al. 2022

IJHP

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Basic Health Research Report shows that an average prevalence of diabetes mellitus (DM) in urban areas is 5.7% in the population aged over 15 years. Many studies have been conducted to explore the pharmacological effects of snake fruit skin as different preparations against DM. In this study, the effect of snake fruit skin extract (SFSE) on body weight and blood glucose levels induced by alloxan was tested on 25 male Wistar rats. All rats were initially induced with 5% alloxan (150 mg/kg BW) then after 48 hours were grouped into: control (Na-CMC 0.5%), standard (Metformin), SFSE-I (60 mg/200 gBW), SFSE -II (120 mg/200 gBW), and SFSE-III (240 mg/200 gBW). The treatment was given for 14 days. The results showed that the tendency for the highest blood glucose levels of about 333 mg/dl (after 28 days of treatment) was found in the control group, followed by SFSE-I (222 mg/dl), -II (205 mg/dl), -III (138 mg/dl), and the lowest in the standard group was about 129 mg/dl. Hence, it can be concluded that SFSE which has the best antihyperglycemic effect was obtained with the highest dose of 240 mg/200 gBW.

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Section: Anti-diabetic Activity Test Of Sfsementioning

confidence: 99%

Anti-Diabetic Effect of Snake Fruit Skin Extract in Alloxan-Induced Wistar Rat

Marzuki

Girsang

Nasution

et al. 2022

IJHP

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In vitro cytotoxic effect of stigmasterol derivatives against breast cancer cells

Dube,

Tembu,

Nyemba

et al. 2023

BMC Complement Med Ther

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Background Stigmasterol is an unsaturated phytosterol that belong to the class of tetracyclic steroids abundant in Rhoicissus tridentata. Stigmasterol is an important constituent since it has shown impressive pharmacological effects such as anti-osteoarthritis, anticancer, anti-diabetic, anti-inflammatory, antiparasitic, immunomodulatory, antifungal, antioxidant, antibacterial, and neuroprotective activities. Furthermore, due to the presence of π system and hydroxyl group, stigmasterol is readily derivatized through substitution and addition reactions, allowing for the synthesis of a wide variety of stigmasterol derivatives. Methods Stigmasterol (1) isolated from Rhoicissus tridentata was used as starting material to yield eight bio-active derivatives (2–9) through acetylation, epoxidation, epoxide ring opening, oxidation, and dihydroxylation reactions. The structures of all the compounds were established using spectroscopic techniques, NMR, IR, MS, and melting points. The synthesized stigmasterol derivatives were screened for cytotoxicity against the hormone receptor-positive breast cancer (MCF-7), triple-negative breast cancer (HCC70), and non-tumorigenic mammary epithelial (MCF-12 A) cell lines using the resazurin assay. Results Eight stigmasterol derivatives were successfully synthesized namely; Stigmasterol acetate (2), Stigmasta-5,22-dien-3,7-dione (3), 5,6-Epoxystigmast-22-en-3β-ol (4), 5,6-Epoxystigmasta-3β,22,23-triol (5), Stigmastane-3β,5,6,22,23-pentol (6), Stigmasta-5-en-3,7-dion-22,23-diol (7), Stigmasta-3,7-dion-5,6,22,23-ol (8) and Stigmast-5-ene-3β,22,23-triol (9). This is the first report of Stigmasta-5-en-3,7-dion-22,23-diol (7) and Stigmasta-3,7-dion-5,6,22,23-ol (8). The synthesized stigmasterol analogues showed improved cytotoxic activity overall compared to the stigmasterol (1), which was not toxic to the three cell lines tested (EC50 ˃ 250 µM). In particular, 5,6-Epoxystigmast-22-en-3β-ol (4) and stigmast-5-ene-3β,22,23-triol (9) displayed improved cytotoxicity and selectivity against MCF-7 breast cancer cells (EC50 values of 21.92 and 22.94 µM, respectively), while stigmastane-3β,5,6,22,23-pentol (6) showed improved cytotoxic activity against the HCC70 cell line (EC50: 16.82 µM). Conclusion Natural products from Rhoicissus tridentata and their derivatives exhibit a wide range of pharmacological activities, including anticancer activity. The results obtained from this study indicate that molecular modification of stigmasterol functional groups can generate structural analogues with improved anticancer activity. Stigmasterol derivatives have potential as candidates for novel anticancer drugs.

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Review of the Traditional Uses, Phytochemistry, and Pharmacological Activities of Rhoicissus Species (Vitaceae)

et al. 2021

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Species within the genus Rhoicissus (Vitaceae) are commonly used in South African traditional medicine. The current review discusses the occurrence, distribution, traditional uses, phytochemistry, and pharmacological properties of Rhoicissus species covering the period 1981–2020. The data reported were systematically collected, read, and analysed from scientific electronic databases including Scopus, Scifinder, Pubmed, and Google Scholar. Reported evidence indicates that species in this genus are used for the treatment of gastrointestinal complaints, sexually transmitted infections (STIs), and infertility, as well as to tone the uterus during pregnancy and to facilitate delivery. Pharmacological studies have further shown that members of the Rhoicissus genus display antidiabetic, uterotonic, ascaricidal, hepatoprotective, antioxidant, antimicrobial, anticancer, and anti-inflammatory properties. They are linked to the presence of bioactive compounds isolated from the genus. Hence, Rhoicissus species can potentially be an alternative therapeutic strategy to treat diseases and develop safer and more potent drugs to combat diseases. Plant species of this genus have valuable medicinal benefits due to their significant pharmacological potential. However, scientific investigation and information of the therapeutic potential of Rhoicissus remain limited as most of the species in the genus have not been fully exploited. Therefore, there is a need for further investigations to exploit the therapeutic potential of the genus Rhoicissus. Future studies should evaluate the phytochemical, pharmacological, and toxicological activities, as well as the mode of action, of Rhoicissus crude extracts and secondary compounds isolated from the species.

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In Vivo Anti-diabetic Effects of Aqueous Leaf Extracts of Rhoicissus tridentata in Alloxan Induced Diabetic Mice

Cited by 5 publications

References 16 publications

Anti-Diabetic Effect of Snake Fruit Skin Extract in Alloxan-Induced Wistar Rat

Anti-Diabetic Effect of Snake Fruit Skin Extract in Alloxan-Induced Wistar Rat

In vitro cytotoxic effect of stigmasterol derivatives against breast cancer cells

Review of the Traditional Uses, Phytochemistry, and Pharmacological Activities of Rhoicissus Species (Vitaceae)

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