In Vivo Anti-Hypercholesterolemia Effect of Indonesian Jamu Formula

Abstract

Indonesia has many Jamu formulas to threat some diseases including for hypercholesterolemia. Aim of this research were to standardize and analyze the effectiveness of Jamu Formula as anti-hypercholesterolemia and see its toxicity to liver function. Jamu Formula in this research consisted of Syzygium polyanthum, Casia alata, and Centella asiatica leaves which were extracted by ethanol 30%, and Curcuma xanthorrhiza rhizome which were extracted by ethanol 70%. This Jamu fomula can inhibit HMGCoA reductase activity in previous study. This study was conducted in animals laboratory of Tropical Biopharmaca Research Center, Bogor, Indonesia. In vivo analysis using Sprague dawley rats fed a high-fat diet for 4 weeks, then treated with Jamu Formula for 2 weeks. Blood collection performed before and after treatment. The results showed that Jamu Formula have a good quality for plant raw material and safe to be used. It had LD50 higher than 5000 mg/kg BW. The Jamu formula dose 360 mg/kg BW can decreased cholesterol level and body weight of rats about1,5 and 2,5 times relative to negative control group. Total cholesterol and LDL level of Jamu formula dose 360 mg/kg BW can decrease to 92.4 ± 5.0 and 25.8 ± 8.6 mg/dL which was not significantly different with normal and simvastatin group. In conclusion, Jamu formula dose 360 mg/kg BW have anti-hypercholesterolemia effect as good as simvastatin group.